LOCUS       sequence-26146-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Cre-activated AAV expression plasmids
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..69)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(120..153)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(165..416)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(207..228)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     556..575
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgtgaggtac cggatcctct agagtcgact ccggaataac ttcgtatagg atactttata
       61 cgaagttatg cagaatggta gctggattgt agctgctatt agcaatatga aacctcttaa
      121 taacttcgta tagcatacat tatacgaagt tatggcgcgc cttacttgta cagctcgtcc
      181 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      241 tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc
      301 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      361 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
      421 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      481 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      541 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      601 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      661 cgggcgaagc actgcaggcc gtagccgaag gtggtcacga nggtggccca gggcacgggc
      721 agcttgccgg tggtgcagat gaacttcagg gtcagctngc ngtaggtggc atcgcctcgc
      781 ctcgcggaca cgctganctg tgccgttact cgcgtcactc gacagaggca caccggggan
      841 actctcgctt gtcc
//
