LOCUS       sequence-261460-        9384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human 33-G-X1 in 293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Qin Y, Garrison BS, Ma W, Wang R, Jiang A, Li J, Mistry M, Bronson 
            RT, Santoro D, Franco C, Robinton DA, Stevens B, Rossi DJ, Lu C, 
            Springer TA
  TITLE     A Milieu Molecule for TGF-beta Required for Microglia Function in 
            the Nervous System.
  JOURNAL   Cell. 2018 Jun 28;174(1):156-171.e16. doi: 
            10.1016/j.cell.2018.05.027. Epub 2018 Jun 14.
  PUBMED    29909984
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.027"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "156-171.e16"
COMMENT     Sequence Label: 33-G-X1-pEF1-puro
FEATURES             Location/Qualifiers
     source          1..9384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2107..3285
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2338..3276
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3233..3253
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3302..3321
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3302..3320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3437..3460
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5458..5499
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5509..5526
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5549..5566)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5555..5779
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5825..6253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5912..5931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6122..6143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6262..6282)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6267..6596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6447..6582
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6509..6528
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             complement(7123..7722)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7206..7226)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7703..7722
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7734..8233)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7782..7801)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8175..8198
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        8363..8574
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(8994..9010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8994..9010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9007..9029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9018..9034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9042..9048,9049..9066,9067..9072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9087..9108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9225..9242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagg cttttgcaaa aagctttgca aagatggata
     2041 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     2101 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     2161 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     2221 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     2281 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     2341 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     2401 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2461 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2521 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2581 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2641 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2701 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2761 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2821 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     2881 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     2941 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3001 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3061 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3121 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3181 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3241 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tagcttggta
     3301 ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta ccgagctcgg
     3361 atccgaattc gccaccatgt ggtggcgcct gtggtggctg ctgctgctgc tgctgctgtg
     3421 gcccatggtg tgggccgact acaaggacga cgacgacaag ctcgactgga ggaacagaag
     3481 cggaacagcc acagcagcct cccaaggagt ctgcaagttg gtgggtggag ccgctgactg
     3541 ccgagggcag agcctcgctt cggtgcccag cagcctcccg ccccacgccc ggatgctcac
     3601 cctggatgcc aaccctctca agaccctgtg gaatcactcc ctccagcctt accctctcct
     3661 ggagagcctc agcctgcaca gctgccacct ggagcgcatc agccgcggcg ccttccagga
     3721 gcaaggtcac ctgcgcagcc tggtactggg ggacaactgc ctctcagaga actacgaaga
     3781 gacggcagcc gccctccacg ccctgccggg cctgcggagg ctggacttgt caggaaacgc
     3841 cctgacggag gacatggcag cgctcatgct ccagaacctc tcctcgctgc ggtccgtgtc
     3901 cctggcgggg aacaccatca tgcggctgga cgactccgtc ttcgagggcc tggagcgtct
     3961 ccgggagctg gatctgcaga ggaactacat cttcgagatc gagggcggcg ctttcgacgg
     4021 cctggctgag ctgacccatc tcaacctctc caggaattcc ctcacctgca tctccgactt
     4081 cagcctccag cagctgcggg tgctagacct gagctgcaac agcatcgagg cctttcagac
     4141 ggcctcccag ccccaggctg agttccagct cacctggctt gacctgcggg agaacaaact
     4201 gctccatttc cccgacctgg ccgcgctccc gagactcatc tacctgaact tgtccaacaa
     4261 cctcatccgg ctccccacag ggccacccca ggacagcaag ggcatccacg caccttccga
     4321 gggctggtca gccctgcccc tctcagcccc cagcgggaat gccagcggcc gccccctttc
     4381 ccagctcttg aatctggatt tgagctacaa tgagattgag ctcatccccg acagctttct
     4441 tgagcacctg acctccctgt gcttcctgaa cctcagcaga aactgcttgc ggacctttga
     4501 ggcccggcgc ttaggctccc tgccctgcct gatgctcctt gacttaagcc acaatgccct
     4561 ggagacactg gaactgggcg ccagagccct ggggtctctg cggacgctgc tcctacaggg
     4621 caatgccctg cgggacctgc ccccatacac ctttgccaat ctggccagcc tgcagcggct
     4681 caacctgcag gggaaccgag tcagcccctg tggggggcca gatgagcctg gcccctccgg
     4741 ctgtgtggcc ttctccggca tcacctccct ccgcagcctg agcctggtgg ataatgagat
     4801 agagctgctc agggcagggg ccttcctcca caccccactg actgagctgg acctttcttc
     4861 caatcctggg ctggaggtgg ccacgggggc cttgggaggc ctggaggcct ccttggaggt
     4921 cctggcactg cagggcaacg ggctgatggt cctgcaggtg gacctgccct gcttcatctg
     4981 cctcaagcgg ctcaatcttg ccgagaaccg cctgagccac cttcccgcct ggacacaggc
     5041 tgtgtcactg gaggtgctgg acctgcgaaa caacagcttc agcctcctgc caggcagtgc
     5101 catgggtggc ctggagacca gcctccggcg cctctacctg caggggaatc cactcagctg
     5161 ctgcggcaat ggctggctgg cagcccagct gcaccagggc cgtgtggacg tggacgccac
     5221 ccaggacctg atctgccgct tcagctccca ggaggaggtg tccctgagcc acgtgcgtcc
     5281 cgaggactgt gagaaggggg gactgaagaa catcaacctc atcatcatcc tcaccttcat
     5341 actggtctct gccatcctcc tcaccacgct ggccgcctgc tgctgcgtcc gccggcagaa
     5401 gtttaaccaa cagtataaag ccaccggtta agcggccgct ctagagggcc cttcgaaggt
     5461 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     5521 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5581 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5641 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5701 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5761 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5821 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6241 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6421 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6481 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6541 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6601 tcccatgtcg aggagcttct gatggaatta gaacttggca aaacaatact gagaatgaag
     6661 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
     6721 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
     6781 atctatagat ctatagatca tgagtgggag gaatgagctg gcccttaatt tggttttgct
     6841 tgtttaaatt atgatatcca actatgaaac attatcataa agcaatagta aagagccttc
     6901 agtaaagagc aggcatttat ctaatcccac cccaccccca cccccgtagc tccaatcctt
     6961 ccattcaaaa tgtaggtact ctgttctcac ccttcttaac aaagtatgac aggaaaaact
     7021 tccattttag tggacatctt tattgtttaa tagatcatca atttccgatg catggggtcg
     7081 tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg
     7141 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     7201 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     7261 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     7321 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     7381 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     7441 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     7501 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     7561 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
     7621 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
     7681 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct ggtcgaaagg
     7741 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat
     7801 gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg
     7861 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
     7921 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
     7981 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
     8041 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
     8101 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
     8161 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
     8221 gcctccccta cccggtagaa ttcctcgacg tatcgataag ctttgcaaag atggataaag
     8281 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     8341 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     8401 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     8461 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     8521 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     8581 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     8641 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     8701 tgggagagtt cgaggccttg cgcttaagga gcccttcgcc tcgtgcttga gttgaggcct
     8761 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     8821 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     8881 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     8941 ccgcgggcgg cgacggtacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccaggaaccg
     9361 taaaaaggcc gcgttgctgg cgtt
//
