LOCUS       sequence-261473-        4528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human WDR34, codon optimised for Sf9 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, 
            Roberts AJ
  TITLE     Structure of the dynein-2 complex and its assembly with 
            intraflagellar transport trains.
  JOURNAL   Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. 
            doi: 10.1038/s41594-019-0286-y.
  PUBMED    31451806
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 
            10.1038/s41594-019-0286-y."
COMMENT     Sequence Label: WDR34
FEATURES             Location/Qualifiers
     source          1..4528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      148..736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     637..656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  871..1094
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1161..1694)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1883..1888,1889..1905,1906..1911))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1982..2015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2130..2221
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2146..2163
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4029..4150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4066..4085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4120..4139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4321..4486
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttgttgta cctatgggca tggcacaatt ctagcgcatg
     2101 ccatcctggg ttactgttca tatcgtgcaa gaatggagat aattaaaatg ataaccatct
     2161 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
     2221 tattccggat tattcatacc gtcccaccat cgggcgcgga tcctcaacat ggcaacaagg
     2281 gctcaacctg gtcctctctc tcaagctgga tctgctggcg tggctgctct ggctaccgtc
     2341 ggagtggctt ctggacccgg tcctggcaga cccggaccac tgcaagacga aaccctcggc
     2401 gtggcttccg tgccttctca gtggagagcc gtccagggaa ttagaggcga aactaagtca
     2461 tgccagactg catcaatcgc taccgcctct gcctccgctc aagccagaaa tcatgtggat
     2521 gctcaggtgc agaccgaggc acctgtccct gtgtcagtgc agcctccatc tcagtatgac
     2581 atccccaggc tcgcagcctt cctgcgcaga gtcgaggcta tggtgatcag ggagctgaac
     2641 aagaactggc aatcccacgc tttcgatgga ttcgaggtca attggactga acagcagcag
     2701 atggttagct gcctgtatac actgggttat cctcctgctc aggctcaggg cctgcacgtc
     2761 acttctatct catggaactc aaccggctca gtcgtcgcct gtgcttacgg taggctggac
     2821 cacggtgact ggtccacact caagtcattt gtctgtgctt ggaacctcga ccgcagggac
     2881 ctgaggccac aacagccaag cgctgtcgtg gaggtcccta gcgctgtcct gtgcctggcc
     2941 ttccatccca cacagccaag ccatgttgct ggtggactgt actctggtga ggtcctggtg
     3001 tgggacctct cacgcctcga agatcccctc ctctggagaa ctggcctgac tgatgacacc
     3061 cacaccgacc cagtgagcca ggtcgtctgg ctcccagaac ccggtcactc ccacaggttt
     3121 caagtcctca gcgtggccac agacggcaag gtcttgttgt ggcagggcat cggcgtcggc
     3181 cagttgcagc tgaccgaagg cttcgccctg gtgatgcaac agctgcctag gtctactaaa
     3241 ctcaagaaac acccaagagg cgaaaccgag gttggtgcta ccgcagttgc cttttcctct
     3301 ttcgatccaa gactcttcat tctgggtact gagggaggct tccctctgaa gtgttccctc
     3361 gctgccggtg aagctgctct gacaaggatg cctagctccg tgcccctgag ggctcctgcc
     3421 cagtttacct tcagcccaca tggtggaccc atctacagcg tgagctgctc tccattccac
     3481 cgcaacctgt ttctgagcgc aggaaccgac ggccacgttc acttgtactc tatgctccaa
     3541 gcacctcccc tgacctcatt gcaactgtct ctgaagtact tgttcgccgt taggtggtcc
     3601 cctgttcgcc cactggtgtt tgccgcagct tccggaaagg gcgatgtcca gctgttcgat
     3661 ttgcagaaat cttctcaaaa gcccacagtt ttgatcaagc agactcaaga cgagagccca
     3721 gtgtattgtt tggagtttaa ctcccagcag acccagctgt tggctgctgg tgatgctcag
     3781 ggtactgtta aagtttggca gttgagcaca gagtttacag aacagggtcc tcgcgaagcc
     3841 gaggatttgg attgcctggc agctgaggtg gctgcatgac tagagcctgc agtctcgaca
     3901 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     3961 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4021 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4141 atgtatctta tcatgtctgg aaacatccac catttaaatg gagttaggtt taaacggatc
     4201 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     4261 ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc atctaattgg
     4321 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
     4381 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
     4441 ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg gttgacttgg
     4501 gtcaactgtc agaccaagtt tactcata
//
