LOCUS       sequence-261476-       17001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised.
            With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage
            site at N-terminus. Assembled from synthesised fragments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17001)
  AUTHORS   Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, 
            Roberts AJ
  TITLE     Structure of the dynein-2 complex and its assembly with 
            intraflagellar transport trains.
  JOURNAL   Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. 
            doi: 10.1038/s41594-019-0286-y.
  PUBMED    31451806
REFERENCE   2  (bases 1 to 17001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 
            10.1038/s41594-019-0286-y."
COMMENT     Sequence Label: His-ZZ-TEV-SNAPf-DYNC2H1
FEATURES             Location/Qualifiers
     source          1..17001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      148..736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     637..656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  871..1094
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1161..1694)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1883..1888,1889..1905,1906..1911))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1982..2015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2145..2162
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2280..2303
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2751..2771
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2793..2813
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2814..3356
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             3363..3386
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3408..3431
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    16502..16623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16539..16558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16593..16612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  16794..16959
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttgaccct tcggcgatgt actgtgtata actgacatat
     2101 gcgtctattt gatgctgtga agataccgcc aatggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg tcttactatc
     2281 accaccacca tcaccaccat cacggttcaa tgtgcatggc tggcctcgcc cagcacgacg
     2341 aagctgttga caataagttc aacaaggaac agcagaacgc tttctacgaa atcctccacc
     2401 tgccaaacct caacgaagaa cagcgcaacg cattcatcca atcactgaag gacgacccat
     2461 cccaatccgc aaacctgttg gccgaagcaa agaagctgaa cgacgctcag gctcccaaag
     2521 tggacaataa gttcaataaa gagcagcaga acgccttcta tgagattctg cacttgccta
     2581 acctgaacga ggaacagcgc aatgctttca tccagtctct gaaggatgat ccatcacaga
     2641 gcgctaattt gctggccgag gccaagaagc tcaatgatgc tcaagctccc aaggtggatg
     2701 ctaactccgc cggaaagtcc actgactacg acatccctac aactgcaagc gagaacctct
     2761 acttccaagg cgactatgac atcccaacta cagagaatct ctatttccag ggtgacaaag
     2821 actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt
     2881 gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg
     2941 tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg caggccaccg
     3001 cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc
     3061 tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga
     3121 aagtggtgaa gttcggagag gtcatcagct acagccacct ggccgccctg gccggcaatc
     3181 ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc
     3241 cctgccaccg ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc gggctcgccg
     3301 tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg ctgggtggtt
     3361 ctcttgaggt gctctttcag ggacccgatt atgatatccc tacgactctt gaggtgctct
     3421 ttcagggacc cgctaacgga accgctgacg tgagaaaact gttcatcttc actaccactc
     3481 agaactactt tggcttgatg agcgaattgt gggatcagcc cctgttgtgc aactgcctcg
     3541 aaatcaacaa tttcctcgat gatggaaacc aaatgctgct gagggtgcag agatccgatg
     3601 ccggaatttc tttctcaaat accatcgagt ttggcgacac aaaggacaag gtgctcgtgt
     3661 tcttcaaact gaggcctgaa gtcatcacag atgaaaacct gcacgacaac atcctggtgt
     3721 cttctatgtt ggagagccct atctcttctc tctatcaagc tgtcaggcag gtgttcgctc
     3781 caatgctgct gaaagaccag gagtggagca gaaactttga tcccaagctc caaaacctgc
     3841 tgagcgaact ggaggcaggt ttgggtatcg tgctgcgcag atcagacact aatctcacta
     3901 agctcaagtt caaggaggac gacaccaggg gcattttgac accctcagac gagtttcagt
     3961 tctggattga gcaagcccac aggggcaata agcagattag caaggaacgc gctaactact
     4021 tcaaggaact gttcgaaacc atcgctagag agttctacaa tctcgactct ttgagcctcc
     4081 tggaggttgt ggacttggtc gaaacaactc aggatgtcgt cgatgacgtg tggaggcaga
     4141 ccgaacacga tcactacccc gaatcaagga tgctccacct gctcgacatc atcggtggtt
     4201 cctttggaag atttgtgcag aagaagctcg gcacactgaa cctgtgggag gacccttact
     4261 acctggtgaa agaatctctg aaggctggca tttctatctg cgagcaatgg gttatcgtct
     4321 gcaatcacct gacaggccag gtgtggcaac gctacgtgcc acatccctgg aagaatgaga
     4381 agtactttcc agaaactctg gataagctcg gtaagcgcct ggaggaggtc ctggctatca
     4441 gaacaatcca cgaaaagttt ctgtactttc tccctgcctc tgaggagaaa atcatctgct
     4501 tgaccagagt cttcgaacct tttacaggtc tgaaccccgt gcagtacaat ccctacactg
     4561 agcctttgtg gaaggcagcc gttagccaat acgaaaagat tatcgctccc gctgagcaga
     4621 aaatcgctgg caagctgaag aattacatca gcgaaattca ggacagccct caacagctgc
     4681 tccaggcatt cctgaagtat aaggaactcg tcaaaagacc tactatctcc aaggaactga
     4741 tgctcgaacg cgagactttg ttggccaggt tggtggactc catcaaagat ttcaggttgg
     4801 actttgagaa taggtgcagg ggtatcccag gtgatgcttc aggacctctg tccggtaaga
     4861 atttgtccga ggtcgtgaac tctatcgtct gggttcgcca gctggaattg aaagtcgatg
     4921 acacaatcaa gatcgctgag gctctgttgt cagacctgcc aggattcaga tgcttccatc
     4981 agagcgctaa ggacctgctg gaccaactca aactgtatga acaagagcag ttcgacgatt
     5041 ggagcagaga catccaatcc ggcttgtctg acagcaggtc cggactgtgt attgaggctt
     5101 cctcaaggat tatggaactc gattctaacg acggcttgct gaaggtgcac tactccgaca
     5161 ggctcgtgat tctcttgcgc gaagtccgcc agttgtctgc cttgggcttc gtgatccctg
     5221 ccaagatcca gcaggtggct aacatcgccc agaaattctg caagcaagct atcatcttga
     5281 agcaagtggc ccacttttac aattccattg atcagcaaat gatccaatcc caacgcccaa
     5341 tgatgctcca gagcgctctc gctttcgagc agatcattaa gaactcaaaa gctggatctg
     5401 gcggtaagag ccaaatcaca tgggacaacc ccaaagagct ggagggctat atccagaaat
     5461 tgcagaacgc tgctgagagg ctggcaacag agaataggaa attgcgcaag tggcatacca
     5521 ccttctgcga gaaagtggtg gtgctgatga acattgactt gctgcgccag cagcagaggt
     5581 ggaaggacgg tttgcaggaa ctcagaactg gactggctac agtcgaagct cagggctttc
     5641 aggccagcga tatgcacgct tggaagcaac actggaacca ccaactgtac aaggcactcg
     5701 aacaccaata tcaaatggga ctggaggctc tcaacgagaa tctgcctgag atcaatatcg
     5761 acctcacata caagcagggt cgcctccaat tcaggccacc tttcgaagag atcagggcca
     5821 aatactacag ggagatgaaa cgcttcatcg gcattccaaa ccagttcaag ggagtgggcg
     5881 aggctggtga tgagtcaatc ttcagcatta tgattgatcg caatgcttcc ggattcttga
     5941 caatcttctc caaggctgaa gacctgttca ggcgcttgtc tgctgtcctg caccaacaca
     6001 aagaatggat cgtcattgga caggtggaca tggaagcctt ggttgaaaag cacctcttta
     6061 ctgtgcatga ctgggaaaag aactttaagg ccctgaagat caaaggaaag gaggtcgaaa
     6121 ggctcccatc agctgtcaag gtggactgcc tgaacattaa ctgcaaccct gtcaagacag
     6181 tcattgacga tctcattcag aaactctttg acttgttggt gctctctctg aagaagtcca
     6241 tccaggctca cttgcacgaa attgacacct ttgtcactga agctatggaa gtgctgacta
     6301 tcatgccaca gagcgtcgag gagatcggcg atgctaacct gcaatactca aagctgcaag
     6361 aacgcaaacc tgaaatcctg ccactgttcc aagaggctga ggacaagaac cgcctgttga
     6421 ggaccgtcgc aggtggaggt ctggagacta tctctaatct gaaagctaag tgggacaagt
     6481 ttgaactcat gatggaatcc caccagttga tgattaagga tcagatcgag gtgatgaagg
     6541 gtaacgtgaa atctcgcttg caaatctact accaggagct ggagaagttc aaagctagat
     6601 gggatcagct caaacccggt gacgacgtga ttgaaactgg ccaacacaac actttggaca
     6661 agtcagccaa attgatcaag gagaagaaga tcgagtttga tgatctggaa gtgacaagaa
     6721 agaagctggt ggacgattgt caccacttca ggctggagga gcccaacttt tctctggctt
     6781 catccatttc aaaagacatc gagtcctgtg cccagatttg ggcattctac gaggagtttc
     6841 agcaaggatt ccaagaaatg gctaatgaag actggatcac tttccgcacc aaaacatacc
     6901 tgttcgagga gttcttgatg aattggcatg acaggctccg caaagttgag gagcattctg
     6961 ttatgacagt taagttgcag tctgaggtgg acaagtacaa gatcgtgatc ccaattctca
     7021 aatacgtcag gggagagcac ttgagcccag atcattggct cgacctgttt aggctgctgg
     7081 gtttgcccag gggcaccagc ctcgaaaagc tgttgttcgg cgacctgttg cgcgtggctg
     7141 atactatcgt ggcaaaagca gccgatttga aggacctgaa ctccagggct caaggtgaag
     7201 tgacaattag agaggcactc agggaattgg acctgtgggg agttggagct gtcttcacac
     7261 tgattgacta cgaagacagc caatctagga ctatgaaact gatcaaggac tggaaggaca
     7321 tcgtgaacca ggttggcgat aacagatgcc tcctgcaaag cctcaaagat tcaccctact
     7381 acaagggttt tgaggacaag gtgagcattt gggagcgcaa gctggccgaa ctggacgaat
     7441 acctccaaaa cctcaaccac attcagagaa agtgggtgta cctcgaaccc atctttggta
     7501 ggggagcact gcccaaggaa cagactcgct tcaatagggt ggatgaggat ttcagatcca
     7561 ttatgaccga catcaagaaa gataaccgcg tgactactct gacaactcat gcaggcatta
     7621 ggaactctct cttgaccatc ctggatcagt tgcagagatg tcagcgctcc ctcaacgagt
     7681 tcctggagga gaagaggtct gcttttccac gcttttactt cattggagac gacgacctcc
     7741 tggagatcct gggccaatcc acaaacccat ctgtcattca atcacacttg aagaagctgt
     7801 ttgccggaat caatagcgtt tgctttgacg agaagtctaa gcacattaca gctatgaaga
     7861 gcctggaggg cgaggtggtg cctttcaaga acaaggtgcc actctctaac aatgtcgaaa
     7921 cctggctgaa tgatctcgcc ttggaaatga agaagacctt ggaacaactg ctgaaggagt
     7981 gtgtcactac cggcagatcc tcccaaggag ccgttgaccc ctccttgttc ccatcccaga
     8041 tcctgtgttt ggctgagcag attaagttta ctgaagacgt tgagaacgct atcaaggacc
     8101 actctctgca tcaaatcgag actcaactcg tcaacaagct ggaacagtac actaacatcg
     8161 acaccagctc cgaggaccct ggaaacaccg agagcggaat cctcgaattg aagctgaaag
     8221 ccctcatcct ggacatcatc cacaatatcg acgttgtcaa gcagctgaat cagatccagg
     8281 tccacactac cgaagactgg gcttggaaga agcaattgag gttttacatg aagtctgatc
     8341 acacttgctg tgttcaaatg gtggattctg agtttcagta cacctacgag tatcagggta
     8401 acgcaagcaa actcgtctat actcccctga ctgacaagtg ctatctgacc ctgacccagg
     8461 ccatgaaaat gggcctgggt ggcaatcctt acggaccagc tggtacaggt aagaccgagt
     8521 cagtcaaagc actcggtgga ttgctgggta gacaagtgtt ggtgttcaat tgcgatgagg
     8581 gaatcgacgt gaagtctatg ggacgcatct tcgtcggtct ggtcaagtgt ggcgcttggg
     8641 gctgcttcga tgagttcaat agactggagg agtctgtcct gagcgcagtc tctatgcaga
     8701 tccaaacaat tcaggacgca ttgaagaacc ataggaccgt gtgcgaattg ctgggtaaag
     8761 aagtcgaggt gaactccaat tcaggtatct tcatcaccat gaatccagca ggaaagggct
     8821 acggaggaag gcagaaattg cctgataatc tgaagcagct ctttagaccc gttgcaatgt
     8881 ctcaccccga caacgagctg attgctgagg tcatcctcta ttccgagggc tttaaggacg
     8941 ctaaggtcct ctccagaaag ctggtggcaa tcttcaacct gtccagggaa ctgttgacac
     9001 ctcagcagca ctacgactgg ggtctgaggg ctctgaagac cgtgttgaga ggatctggaa
     9061 atctcttgag acagctcaac aaaagcggaa ccacacaaaa cgcaaacgaa tcccatattg
     9121 tggttcaggc actccgcctg aatactatga gcaagttcac ttttactgat tgtactcgct
     9181 tcgatgctct gatcaaggat gtgttccctg gaatcgagct gaaagaagtg gaatacgacg
     9241 aactctcagc cgcattgaaa caagtgtttg aggaggcaaa ctacgagatc atccctaatc
     9301 agatcaagaa agccttggaa ctctacgaac aactgtgtca gcgcatgggt gtcgtgatcg
     9361 ttggaccctc tggtgctggt aagtcaactc tgtggagaat gctgagagcc gctctctgca
     9421 aaacaggaaa ggtggtgaag cagtacacca tgaacccaaa agccatgcca cgctaccagt
     9481 tgctcggtca cattgacatg gataccagag aatggtccga cggcgtcctg actaacagcg
     9541 ctcgccaggt ggttcgcgag ccacaggacg tgagcagctg gatcatctgc gacggcgata
     9601 ttgaccctga gtggatcgag tccctgaact ccgtgttgga cgacaatagg ttgttgacaa
     9661 tgccctctgg cgagcgcatc caattcggac ccaatgtgaa cttcgtcttc gaaacccacg
     9721 acttgagctg tgcttctccc gctaccatct cccgcatggg aatgattttc ttgtccgacg
     9781 aggagacaga cctcaacagc ctgatcaagt cttggttgag aaaccagcct gctgagtaca
     9841 gaaacaacct ggagaactgg atcggtgact acttcgagaa agctctccag tgggtcctga
     9901 aacagaatga ctatgtcgtg gagacatccc tggttggtac agtcatgaac ggactctctc
     9961 acctgcacgg atgcagggac cacgacgagt ttatcattaa cctgatcagg ggactcggag
    10021 gcaacctgaa tatgaagtca agactggagt tcactaagga agtgtttcat tgggccagag
    10081 aatcacctcc cgatttccat aaacctatgg acacctacta cgactccact aggggacgct
    10141 tggctactta cgtgctcaag aagccagaag acctgaccgc tgatgacttc agcaacggcc
    10201 tgaccctgcc cgttatccaa actccagata tgcaacgcgg tctggactat ttcaaaccct
    10261 ggctgtcctc cgataccaaa cagccattca tcctcgtggg tcccgaaggc tgcggaaaag
    10321 gaatgctgct caggtacgca ttttcccaac tccgctctac ccaaatcgct acagtgcatt
    10381 gctcagctca aacaactagc aggcacctgc tgcaaaagct ctcccaaaca tgcatggtga
    10441 tttctaccaa caccggcagg gtctatagac caaaggattg cgaacgcctg gtcctgtact
    10501 tgaaagacat caacctccct aagttggaca agtggggcac ttcaactctc gtggcttttc
    10561 tccagcaagt tctcacctat caaggtttct atgatgaaaa cttggagtgg gtcggattgg
    10621 agaatatcca gatcgtggcc tcaatgtctg ccggaggcag gttgggaaga cacaaactga
    10681 ccacacgctt cactagcatc gtgcgcctct gttctatcga ttatcctgag agagagcaat
    10741 tgcagaccat ctacggagcc tacctggaac ccgttctgca caagaacctg aagaaccaca
    10801 gcatctgggg aagctccagc aaaatctact tgctggctgg atctatggtg caggtgtacg
    10861 agcaggttag ggccaaattc accgtggacg attacagcca ttacttcttc actccttgca
    10921 ttttgaccca gtgggtgctc ggtttgttcc gctacgattt ggagggaggc tcatcaaacc
    10981 acccactcga ctacgtgttg gagatcgttg catacgaagc taggaggttg ttcagggaca
    11041 agatcgtggg agccaaagaa ctgcacctgt tcgacatcat cctgacctct gtcttccaag
    11101 gcgattgggg atctgacatc ctggataaca tgtctgactc cttctatgtg acctggggag
    11161 caagacataa ctccggtgct cgcgctgccc caggtcagcc cctgccacca cacggtaaac
    11221 ctctgggtaa gctgaacagc accgacctga aagatgttat caagaaaggc ctgatccatt
    11281 acggacgcga caaccaaaac ctggatattc tcctcttcca cgaggtcctg gaatacatga
    11341 gcagaattga tagagtgctg tcctttcccg gaggcagcct gctgctggca ggaagaagcg
    11401 gagtcggtcg cagaaccatc acaagcctcg tttcccacat gcatggtgca gtcctcttct
    11461 ctcctaagat ttcaagaggt tatgaactca aacagtttaa gaatgacctc aagcatgtcc
    11521 tccagctggc tggaatcgag gcccagcagg tcgtcctgct cctggaagac taccagttcg
    11581 tccatccaac attcctggag atgatcaatt cattgctcag ctccggagag gtgccaggtc
    11641 tgtataccct ggaggagctg gaacccctcc tgctgcctct gaaggaccag gcttcacagg
    11701 acggattctt cggtccagtc ttcaactact tcacataccg cattcaacag aatctgcata
    11761 ttgtcctgat catggactct gcaaattcaa acttcatgat taactgtgaa tccaacccag
    11821 ctctccataa gaaatgccaa gtgctgtgga tggaaggatg gagcaatagc tctatgaaga
    11881 agattcccga gatgctgttc tctgaaacag gtggcggaga gaagtacaat gacaagaaga
    11941 gaaaggaaga aaagaagaag aactccgtgg accccgattt tctcaagtct tttctcctga
    12001 tccatgaatc ttgcaaggct tatggtgcca ccccttccca gtacatgact tttctccacg
    12061 tctattctgc tatctcctct agcaagaaga aggaactgct gaagcgccaa tcccatctcc
    12121 aagcaggcgt tagcaagctg aatgaagcaa aggccttggt ggacgagttg aaccgcaaag
    12181 ctggagaaca gagcgttctc ctcaagacca aacaggacga agctgatgcc gctttgcaaa
    12241 tgatcaccgt ctcaatgcag gatgcttccg aacagaaaac tgaactggag agattgaagc
    12301 accgcatcgc cgaggaagtt gtgaaaatcg aggaaaggaa gaacaaaatc gatgatgagc
    12361 tgaaggaggt gcaacctctg gttaatgaag ccaagctggc cgtgggtaac atcaagccag
    12421 agtctctcag cgagattagg tccttgagaa tgcctccaga cgtgatcagg gacatcctgg
    12481 aaggcgtgct caggctcatg ggaatcttcg acacttcttg ggtgtccatg aagagcttct
    12541 tggcaaaacg cggtgttagg gaagacatcg ctacctttga tgcaaggaac atctcaaagg
    12601 aaattcgcga gtccgttgag gaactcctct ttaagaacaa gggttcattc gatcccaaga
    12661 atgctaaacg cgcttctact gctgccgctc ccttggcagc ctgggtgaag gccaatatcc
    12721 agtactctca tgtgctggaa agaatccacc cactggaaac tgagcaagct ggactggagt
    12781 caaatctgaa gaaaaccgaa gataggaaaa gaaaactgga ggagctgttg aactcagtgg
    12841 gacagaaagt gtccgagctg aaggaaaagt tccagtccag aacttcagaa gctgccaaac
    12901 tggaagcaga ggtcagcaag gctcaggaaa caatcaaagc cgcagaagtg ttgattaacc
    12961 agctggaccg cgaacacaaa agatggaacg cacaggtggt ggaaatcact gaagaactgg
    13021 ccaccctgcc aaagagagca caactcgcag ctgctttcat tacatatctc tcagccgctc
    13081 cagaatctct gagaaagact tgcctggaag agtggaccaa atctgccgga ctggaaaagt
    13141 ttgacctccg caggttcctg tgtactgaat ccgagcagct catctggaag tctgagggtc
    13201 tgccttctga cgatctgtcc atcgaaaacg ccctggtcat tttgcagtcc agagtctgcc
    13261 cttttctgat cgatccaagc tcccaggcta ccgagtggct gaaaacacat ctcaaagact
    13321 caagactgga ggtgatcaac cagcaagact ccaacttcat tactgctctg gagctggctg
    13381 tgaggttcgg caagaccctc attatccagg aaatggatgg tgtcgagcct gttctctatc
    13441 ccctgctccg cagggacctg gttgctcagg gtccaaggta cgtggtccag atcggcgata
    13501 agatcattga ttacaatgaa gagttccgcc tctttctgtc aactagaaac ccaaacccct
    13561 tcatccctcc agacgctgcc tcaattgtga cagaggtgaa tttcaccact acacgctctg
    13621 gcctgagagg acagctcctg gccctgacca tccagcacga gaaacctgat ctcgaagagc
    13681 agaaaactaa gttgctgcaa caagaggaag ataagaagat ccaattggct aagttggagg
    13741 aaagcctctt ggagactctg gcaacttcac agggaaacat cttggaaaac aaggacctga
    13801 tcgagagcct gaaccagaca aaggcaagca gcgcactgat ccaggagtcc ttgaaggaaa
    13861 gctacaaact gcaaatcagc ctggaccagg agagagacgc ctacttgcct ctggcagaat
    13921 ccgcctccaa aatgtatttc attatcagcg acctgtctaa gatcaacaac atgtataggt
    13981 tctctctcgc agcctttctg agattgttcc aaagagccct ccaaaacaag caggacagcg
    14041 aaaacactga gcaacgcatc caatctctga tttcctcact gcaacacatg gtgtatgaat
    14101 acatttgtag atgtctgttc aaggcagacc agctcatgtt cgccctgcac tttgtgaggg
    14161 gtatgcatcc agaactcttt caagagaatg agtgggacac ttttacaggt gtcgtcgtcg
    14221 gagacatgct ccgcaaggcc gacagccaac agaaaatccg cgaccagctg ccctcctgga
    14281 ttgaccagga gcgcagctgg gcagtcgcaa cactcaagat cgctttgccc tcactctatc
    14341 agaccctctg ttttgaggac gcagccttgt ggcgcaccta ttacaacaat tccatgtgcg
    14401 agcaggagtt tccctccatc ctggccaaga aggtgtctct cttccaacaa attctcgtgg
    14461 ttcaggtcct gaggcctgat agactgcaat cagctatggc cctgttcgca tgtaaaaccc
    14521 tgggcctcaa ggaagtctcc ccactgccac tgaatctgaa gaggctctac aaagaaactc
    14581 tggagatcga gcccattttg attatcatct ccccaggtgc agatccctct caggagttgc
    14641 aagagctggc taacgcagag aggtcaggcg aatgctacca tcaggtcgct atgggccagg
    14701 gccaggctga cctggcaatt cagatgctga aagaatgcgc tcgcaacggc gactggctgt
    14761 gtttgaagaa tctgcacctg gtcgtttctt ggctgccagt gctggagaag gaactgaaca
    14821 ccttgcagcc aaaggatacc ttcagattgt ggctgactgc cgaggttcac cccaacttca
    14881 caccaatcct gttgcaatct agcctcaaga tcacctacga atcccctcca ggtctgaaga
    14941 agaacctcat gagaacttac gagtcatgga ctcctgaaca aatctcaaag aaagataaca
    15001 cccacagggc tcatgctctg tttagcctcg cctggtttca cgctgcatgt caggaaagaa
    15061 gaaactacat cccacagggt tggaccaaat tctacgagtt ctcattgtcc gatttgaggg
    15121 caggttacaa tatcatcgac agactgtttg acggagcaaa agatgtgcag tgggagttcg
    15181 ttcacggtct gctcgaaaat gctatctacg gaggtaggat tgacaactat ttcgatctgc
    15241 gcgtcctgca aagctacttg aagcagttct tcaactcctc tgtgatcgac gtgttcaacc
    15301 agagaaacaa gaagagcatt ttcccctact cagtgtctct gcctcagtcc tgtagcatcc
    15361 tggactatag ggcagtgatt gaaaagatcc ccgaagacga caaaccctca ttctttggac
    15421 tgcctgcaaa catcgcaaga tcctcacagc gcatgatctc atctcaggtg atttctcagt
    15481 tgaggattct cggtaggtcc atcactgctg gttccaagtt cgaccgcgaa atctggtcta
    15541 acgagttgtc tcctgtcttg aacctctgga agaaactcaa ccaaaactct aacctgattc
    15601 accaaaaggt gcctccaccc aacgacaggc agggctcacc catcctctct ttcatcatcc
    15661 tggaacagtt caacgctatc aggctggtgc aatctgtcca tcagtccctg gctgccctga
    15721 gcaaggttat cagaggtaca acactgctca gctcagaagt tcaaaagctg gcctctgccc
    15781 tgttgaatca gaaatgtcca ctggcctggc aaagcaaatg ggaaggccca gaagatcccc
    15841 tgcaatatct cagaggactg gttgccagag ccctcgctat tcagaactgg gtcgataagg
    15901 ctgaaaagca ggccttgttg tctgagactc tggacttgtc agagctgttc caccctgata
    15961 ccttcctgaa cgcactccgc caagaaactg cacgcgcagt gggtagatcc gtggactctt
    16021 tgaaattcgt tgcatcttgg aagggtcgct tgcaagaagc aaagctccag attaagatca
    16081 gcggattgct gctggagggc tgtagctttg acggtaacca actgagcgag aatcaactgg
    16141 attcaccttc tgtctcctca gttctgcctt gcttcatggg ttggattcca caagacgctt
    16201 gtggaccata ttctcctgac gaatgcatta gcctccccgt ctacactagc gcagagcgcg
    16261 acagggttgt gaccaacatc gacgttcctt gcggaggtaa ccaggaccag tggattcaat
    16321 gcggagcagc actgttcctc aagaatcagt aactagagcc tgcagtctcg acaagcttgt
    16381 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
    16441 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    16501 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    16561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    16621 ttatcatgtc tggaaacatc caccatttaa atggagttag gtttaaacgg atctgatcac
    16681 tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
    16741 gccaccgctg agcaataact atcataaccc ctagggtata cccatctaat tggaaccaga
    16801 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
    16861 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
    16921 tccacccctc ccagttccca actattttgt ccgcccacaa ccggttgact tgggtcaact
    16981 gtcagaccaa gtttactcat a
//
