LOCUS       sequence-26149-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  TITLE     Cre-activated AAV expression plasmids
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(132..165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(452..471)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttctttcggt gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata
       61 ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat
      121 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gccttacttg
      181 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
      241 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      301 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
      361 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
      421 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
      481 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      541 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgc
//
