LOCUS       sequence-261492-       10366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human reduced folate transporter 1 SLC19A1 for lentiviral 
            transduction, copGFP transduction marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Xiang JS, Kaplan M, Dykstra P, Hinks M, McKeague M, Smolke CD
  TITLE     Massively parallel RNA device engineering in mammalian cells with 
            RNA-Seq.
  JOURNAL   Nat Commun. 2019 Sep 23;10(1):4327. doi: 10.1038/s41467-019-12334-y.
  PUBMED    31548547
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-23"; volume: "10(1):4327. doi"; pages: " 
            10.1038/s41467-019-12334-y"
COMMENT     Sequence Label: pCS4134
FEATURES             Location/Qualifiers
     source          1..10366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1932..2156
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2238..2900)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        complement(2945..3444)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2993..3012)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3386..3409
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        3458..4636
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3689..4627
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4584..4604
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4643..4652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6445..7033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6498..6518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6916..6927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7107..7340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7412..7533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7449..7468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7503..7522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7741..7757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7741..7757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7754..7776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7789..7795,7796..7813,7814..7819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7834..7855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7972..7989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8143..8731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8902..9693,9694..9762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9525..9544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9763..9867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9935..9953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9991..10013)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10113..10132
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10326..10348
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10340..10357
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10341..10357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatctcga gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     1981 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     2041 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     2101 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcgc
     2161 tcgagttagc gagatccggt ggagccgggt ccggcggtgc cgtccacggc agaattggac
     2221 gactgagcgc gggatctggc gaaggcgatg ggggtcttga aggcgtgctg gtactccacg
     2281 atgcccagct cggtgttgct gtgcagctcc tccacgcggc ggaaggcgaa catggggccc
     2341 ccgttctgca ggatgctggg gtggatggcg ctcttgaagt gcatgtggct gtccaccacg
     2401 aagctgtagt agccgccgtc gcgcaggctg aaggtgcggg cgaagctgcc caccagcacg
     2461 ttatcgccca tggggtgcag gtgctccacg gtggcgttgc tgcggatgat cttgtcggtg
     2521 aagatcacgc tgtcctcggg gaagccggtg cccaccacct tgaagtcgcc gatcacgcgg
     2581 ccggcctcgt agcggtagct gaagctcacg tgcagcacgc cgccgtcctc gtacttctcg
     2641 atgcgggtgt tggtgtagcc gccgttgttg atggcgtgca ggaaggggtt ctcgtagccg
     2701 ctggggtagg tgccgaagtg gtagaagccg tagcccatca cgtggctcag caggtagggg
     2761 ctgaaggtca gggcgccttt ggtgctcttc atcttgttgg tcatgcggcc ctgcttgggg
     2821 gtgccctctc cgccgcccac cagctcgaac tccacgccgt tcagggtgcc ggtgatgcgg
     2881 cactcgatct ccatggcggg caggccgctc tcgtcgctct ccatggtaag cttgggctgc
     2941 aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa
     3001 gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc
     3061 ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc
     3121 aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt
     3181 ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga
     3241 gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc
     3301 caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga
     3361 ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc
     3421 ttgggaaaag cgcctcccct acccggtaga agctagcggc tccggtgccc gtcagtgggc
     3481 agagcgcaca tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaaccgg
     3541 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3601 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     3661 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     3721 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     3781 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     3841 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     3901 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     3961 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     4021 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     4081 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     4141 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     4201 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     4261 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     4321 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     4381 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgacct tttggagtac
     4441 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     4501 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     4561 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     4621 atttcaggtg tcgtgatcta gagccaccat ggtgccctcc agcccagcgg tggagaagca
     4681 ggtgcccgtg gaacctgggc ctgaccccga gctccggtcc tggcggcacc tcgtgtgcta
     4741 cctttgcttc tacggcttca tggcgcagat acggccaggg gagagcttca tcacccccta
     4801 cctcctgggg cccgacaaga acttcacgcg ggagcaggtc acgaacgaga tcacgccggt
     4861 gctgtcgtac tcctacctgg ccgtgctggt gcccgtgttc ctgctcaccg actacctgcg
     4921 ctacacgccg gtgctgctgc tgcaggggct cagcttcgtg tcggtgtggc tgctgctgct
     4981 gctgggccac tcggtggcgc acatgcagct catggagctc ttctacagcg tcaccatggc
     5041 cgcgcgcatc gcctattcct cctacatctt ctctctcgtg cggcccgcgc gctaccagcg
     5101 tgtggccggc tactcgcgcg ctgcggtgct gctgggcgtg ttcaccagct ccgtgctggg
     5161 ccagctgctg gtcactgtgg gccgagtctc cttctccacg ctcaactaca tctcgctggc
     5221 cttcctcacc ttcagcgtgg tcctcgccct cttcctgaag cgccccaagc gcagcctctt
     5281 cttcaaccgc gacgaccggg ggcggtgcga aacctcggct tcggagctgg agcgcatgaa
     5341 tcctggccca ggcgggaagc tgggacacgc cctgcgggtg gcctgtgggg actcagtgct
     5401 ggcgcggatg ctgcgggagc tgggggacag cctgcggcgg ccgcagctgc gcctgtggtc
     5461 cctctggtgg gtcttcaact cggccggcta ctacctggtg gtctactacg tgcacatcct
     5521 gtggaacgag gtggacccca ccaccaacag tgcgcgggtc tacaacggcg cggcagatgc
     5581 tgcctccacg ctgctgggcg ccatcacgtc cttcgccgcg ggcttcgtga agatccgctg
     5641 ggcgcgctgg tccaagctgc tcatcgcggg cgtcacggcc acgcaggcgg ggctggtctt
     5701 ccttctggcg cacacgcgcc acccgagcag catctggctg tgctatgcgg ccttcgtgct
     5761 gttccgcggc tcctaccagt tcctcgtgcc catcgccacc tttcagattg catcttctct
     5821 gtctaaagag ctctgtgccc tggtcttcgg ggtcaacacg ttctttgcca ccatcgtcaa
     5881 gaccatcatc actttcattg tctcggacgt gcggggcctg ggcctcccgg tccgcaagca
     5941 gttccagtta tactccgtgt acttcctgat cctgtccatc atctacttct tgggggccat
     6001 gctggatggc ctgcggcact gccagcgggg ccaccacccg cggcagcccc cggcccaggg
     6061 cctgaggagt gccgcggagg agaaggcagc acaggcactg agcgtgcagg acaagggcct
     6121 cggaggcctg cagccagccc agagcccgcc gctttcccca gaagacagcc tgggggctgt
     6181 ggggccagcc tccctggagc agagacagag cgacccatac ctggcccagg ccccggcccc
     6241 gcaggcagct gaattcctga gcccagtgac aaccccttcc ccctgcactc tgtgctccgc
     6301 ccaagcctca ggccctgagg ctgcagatga gacttgtccc cagctggctg tccatcctcc
     6361 tggtgtcagc aagctgggtt tgcagtgtct tccaagcgac ggtgttcaga atgtgaacca
     6421 gtgaggatcc gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6481 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6601 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6661 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6721 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6781 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6841 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6901 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6961 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7021 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     7141 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7201 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7261 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7321 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7381 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9901 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9961 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10021 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10081 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10141 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10201 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10261 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10321 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
