LOCUS       sequence-261515-       12991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Catalytically inactive dCasRx with 2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD
  TITLE     Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR 
            Effectors.
  JOURNAL   Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 
            10.1016/j.cell.2018.02.033.
  PUBMED    29551272
REFERENCE   2  (bases 1 to 12991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2018 
            Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033."
COMMENT     Sequence Label: pXR002: EF1a-dCasRx-2A-EGFP
FEATURES             Location/Qualifiers
     source          1..12991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    60..284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     273..290
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             316..496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1001..1017
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1002..1018
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1021..1609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1127..1138
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1536..1556
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1634..2347,2348..2350))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1637..2347,2348..2350))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1679..1700)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2028..2047
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2287..2308
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2351..2404)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2420..2446)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2456..2476)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2486..5383)
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             complement(5393..5413)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5432..6610)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5441..6379
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5464..5484)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6735..6852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7150..7194)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7379..7612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8105..8230
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8277..8457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8475..8673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8500..8520)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8675..9054
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9226..9245
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9320..9424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9425..9493,9494..10285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9643..9662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10456..11044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10945..10964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11198..11215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11332..11353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11368..11373,11374..11391,11392..11398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11406..11422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11411..11433
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11430..11446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11430..11446
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    11495..11616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11506..11525)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11560..11579
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11746..12120)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(12139..12186)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(12234..12563)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12248..12383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12302..12321)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     12548..12568
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(join(12577..12991,1..14))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12687..12708)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12899..12918
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc
       61 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
      121 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
      181 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
      241 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
      301 ggtttaaacg ggccctgcta gagattttcc acactgacta aaagggtctg agggatctct
      361 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
      421 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
      481 ctaaccagag agacccagta cagtccggat gcagctctcg ggccatgtga tgaaatgcta
      541 ggcggctgtc aaacctccac tctaatactt ctctctccgg gtcatccatc ccatgcaggc
      601 tcacagggtg taacaagcgg gtgttctctc cttcattggc ttcttctacc ttctcttgct
      661 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgatc cctggccctg
      721 gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
      781 tatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
      841 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg
      901 gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg
      961 ctgtattgct acttgtgatt gctccatgtt tttctaggtc tcgaggtcga cggtatcgat
     1021 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     1081 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     1141 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     1201 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     1261 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     1321 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     1381 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     1441 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     1501 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     1561 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
     1621 tgatatcgaa ttcttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     1681 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     1741 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     1801 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     1861 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     1921 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     1981 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     2041 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     2101 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     2161 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     2221 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     2281 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     2341 ccttgctcac tgggccagga ttctcctcga cgtcaccgca tgttagcaga cttcctctgc
     2401 cctctccact gccgctagca gcgtaatctg gaacatcgta tgggtaagcg gccgccacct
     2461 tcctcttttt cttaggtccg gatccggaat tgccggacac cttctttttc tccttgtcga
     2521 acttggcggc ctcgttccta tcgaacaggg cctcgatgct caggttctta aacctgggga
     2581 tacagtagcc gaaaggcaca cacagcagtt tcaggagcct atcgttgtac ttcttctcgt
     2641 cattcacagc gtcgaagtac tcggacacct ttccgctgct tttctcgtac ctctcattca
     2701 tgataattct ctgcatgatg taatggtaca gttggaagta ggaattgacc tcggcaatgt
     2761 cgttgatata ggcgtggaca tacctggcca cttccagggc gacggccttg tttgcgaaca
     2821 gggtacatgt cttgttgtca attctcagga ggtcctccct gatgatcaca ttccacttgg
     2881 tgttcctcag gtaggcgttc agggcggttt tggccttctc cctgttaatc tgcctggtga
     2941 actcctcggc tttcttctcg tcgctgtatt tgatgtaatt ggcatacagc ttggggttgg
     3001 cgctctcgag gctgtcaatg ctctccttgg ctctttcagc catctccttt tccacgtcct
     3061 ttctcttatc gggggcagtt tcatcaatgc cagcgcagag cttggtgacg gagctgaatc
     3121 ccttctcttc cagtttcttg agattgatgt cgtagccttt ctccttgtac agttgagcat
     3181 cacgctcgac gcaatggaat ccgatgacgt acctggcgtt gatattgaca atattcttga
     3241 ggatgtggta gatcacggtg aggtacaggc tgatgatctt tttaaacttc tccctctcgg
     3301 cgttttccct gccggtgtcc tcaatgacgc tccttttctt gtcgaattgg tcgtagttca
     3361 ttccggtgat gatctttgtg agagcgtcca ccttttcgct cacgctcttg cccttatcct
     3421 ttccgataca agtttcgtag tacctgtcga tctggttctt gccgttctgg ccctgttttt
     3481 tctggatgtc agcgatcctg ccgagcacga acttcaccac ggcctcgttt ttggcgatct
     3541 catggaggtg ggcaggatca ccgtatctga tcaggtagtg gaacctttta ttgctgatca
     3601 cgttattaat aatgaaattt ctcatgccgt gcttgccttt cttgagcttg tttccgttct
     3661 cgtccaggga aaaggtgtcg gcgagggcct tgagctcatc ataggacagg ttggttccta
     3721 aaatacggat ggcgtcgata tacatggccc tcctggcatc ggcaataggt tctcccattc
     3781 tagcgaagga cttgatcagc ctcagctcat cggcgatctt ggcggagtct ttgaaaaagg
     3841 cgtattcctc cacgaactta gcgttgactc cgatgagagg catcaccttc aggaagctct
     3901 ggatgttatc gaatttatta atcagggtgg tcaggaggtc gttgatctcc ttgccatcca
     3961 ggaacatggt cagggcatac atgagtttgc tgaaggcgga aacatcacgg ccagcgggca
     4021 ggattctggg cagtctaggg gcgtccttct tcttatactc tcttgtcttg tttcccctaa
     4081 attccttgat gttgtgcatg atattttcga gctttctcca aattctatta gcttcatcgt
     4141 agtagagggc atccttctgg tcgtcgttga aggagcccct caggttaatc acaaagatat
     4201 ccttctcgct cagggacttc tcattatcgg ggagggactt attggcggca gccaccttgg
     4261 catcctcttc gatgtaatac ctataaatca caaagtccat catggtgtag accttggtcc
     4321 tgatggagtc gaacacctta tgatttttcc tgatctcgga catatccttc ctgtccagca
     4381 tcacttccct gagcttggtg atattgaatc cgaggttttt ctgctctttc ataatgctga
     4441 atctgaaata ttgttcggcg aattcggcag ggttgattcc cagagtttcg gcaatatagt
     4501 tcacgttggc ggcggagttc ttggagaagg agttggtcag ctcattggtg atcctgtcgt
     4561 agaggtagtt gagggtggag atgtattcgt tgtcgaggtt cttatcgagg ttgtagagcc
     4621 aggtcctgga gatcctggac tcttcttcgt tgttagcgac cacccagtgc gccagtccgc
     4681 tcaggagggc cagaatgtca tagcattcgt tgccgtaatt gatgatgtaa tttctgccct
     4741 ccttgctgaa aaaggcctgg ccgaaatagc cgagtctggg gttatcgagg aagttgtcga
     4801 actcgtcata ctgggccttg atggcgttga tgagcttatc gttattgttg aaagcggccc
     4861 tatggtgctc ggggtctttg aattcgtcgt aggtatacac tgtggagaac ttgccgaatc
     4921 caataatgtc cttatccagg ccggagatat tgttgacggc gtaggcggcg ttggtaatgt
     4981 attcggcgag gattttttca atgtccagga tgttatggat cacctggata caaatattgt
     5041 cattgccatc agcgctctcg ccgaagtacc tcttttccag agtttccttc aggccgagca
     5101 tatcctgctg gacgggtcct gtatacagag ggttgttagc caccacggcg tagcccttag
     5161 gatggctgaa tttggcgttg ccgatcttat agccggcgtt tttatcggcc atttcagcgc
     5221 tgaaggcctc gccctcattc acgctcctga tgctgtcgcc ctccacgatc ttttccagcc
     5281 tggcgtcgct gccttcggcg aaggttgtca tgtacacttt ggagccggac acgagtgtgg
     5341 acttcacgcc catgcccttg gcgaaggact tttttttttc gatgctggcc tccacctttc
     5401 tcttcttctt ggggctcatg gtggccgtac gtcacgacac ctgaaatgga agaaaaaaac
     5461 tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt
     5521 ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt
     5581 ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc
     5641 tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga
     5701 cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg
     5761 agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg
     5821 ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg
     5881 gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg
     5941 gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt
     6001 cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa
     6061 gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag
     6121 acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc
     6181 agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc
     6241 tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc
     6301 aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca ggcccgcggg
     6361 aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca
     6421 cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt
     6481 acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc
     6541 cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg
     6601 caccggagcc aattcccact cctttcaaga cctagaaggt ccattagctg caaagattcc
     6661 tctctgttta aaactttatc catctttgca ttaattaacc aaactggatc tctgctgtcc
     6721 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     6781 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     6841 tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg cacgcagtgc
     6901 cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
     6961 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
     7021 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
     7081 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
     7141 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
     7201 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
     7261 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
     7321 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
     7381 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
     7441 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
     7501 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
     7561 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
     7621 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     7681 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     7741 tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt ttgttttgct
     7801 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
     7861 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
     7921 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     7981 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     8041 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     8101 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     8161 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     8221 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     8281 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     8341 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     8401 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
     8461 acaggcaaaa cgcgctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     8521 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     8581 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     8641 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     8701 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     8761 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     8821 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     8881 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     8941 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     9001 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg
     9061 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca
     9121 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag
     9181 cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga
     9241 ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg
     9301 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9361 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9421 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9481 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9541 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9601 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9661 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9721 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9781 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9841 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9901 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9961 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10021 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10081 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10141 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10201 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10261 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10321 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10561 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11041 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    11161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11221 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11281 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11341 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11401 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    11461 gctctagcta gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa
    11521 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
    11581 ttatttgtaa ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat
    11641 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
    11701 cctttcatag aaggcggcgg tggaatcgaa atctcgtagc acgtgtcagt cctgctcctc
    11761 ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg
    11821 ctgctcgccg atctcggtca tggccggccc ggaggcgtcc cggaagttcg tggacacgac
    11881 ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc cacacccagg ccagggtgtt
    11941 gtccggcacc acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac
    12001 accggcgaag tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg tccagaactc
    12061 gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg gcactggtca acttggccat
    12121 ggtttagttc ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt
    12181 gtcaacacgt gctgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa
    12241 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg
    12301 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
    12361 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
    12421 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
    12481 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
    12541 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa
    12601 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
    12661 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
    12721 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
    12781 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
    12841 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
    12901 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
    12961 taaccaccac acccgccgcg cttaatgcgc c
//
