LOCUS       sequence-261535-       12417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium  T-DNA  with SpunHsp70pr driving hph-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 12417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM09
FEATURES             Location/Qualifiers
     source          1..12417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2611..2658)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3031..3049
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3254..3474
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3475..4278
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    4443..4467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4648..4670
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4662..4679
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4663..4679
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5766..6791
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5766..6791
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6810..7520
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6837..6865)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7460..7480
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      7543..7730
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7598..7622)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7745..7761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7745..7761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7758..7780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7769..7785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7793..7799,7800..7817,7818..7823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7838..7859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8036..8060
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(8342..8361)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8353..9168
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8353..9168
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8426..8445)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9263..9851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9752..9771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10005..10022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10037..10177
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      10521..10715
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10781..11848)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(join(12282..12417,1..494))
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
       61 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
      121 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
      181 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
      241 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
      301 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
      361 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
      421 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
      481 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
      541 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
      601 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
      661 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
      721 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
      781 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
      841 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
      901 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
      961 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     1021 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     1081 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     1141 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     1201 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     1261 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     1321 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     1381 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     1441 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     1501 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     1561 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
     1621 ctgcactgaa cgtcagaagg aaaagtgcca cctgcatctg tgcttcattt tgtagaacaa
     1681 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     1741 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     1801 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     1861 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     1921 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     1981 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     2041 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     2101 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     2161 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     2221 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     2281 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     2341 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     2401 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     2461 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     2521 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     2581 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
     2641 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
     2701 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
     2761 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
     2821 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
     2881 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
     2941 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
     3001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     3061 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     3121 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     3181 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3241 cataccacag cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg
     3301 tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
     3361 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
     3421 gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg
     3481 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
     3541 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
     3601 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
     3661 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
     3721 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
     3781 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
     3841 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
     3901 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     3961 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     4021 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     4081 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
     4141 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     4201 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     4261 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     4321 attagagctt caatttaatt atatcagtta ttaccggcat gcacatacaa atggacgaac
     4381 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
     4441 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
     4501 acaatctgat ccaagctcaa gctcattcgc cattcaggct gcgcaactgt tgggaagggc
     4561 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4621 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     4681 ccaagctttt ttaaaatctt gtctttgtgc actcttctct ccctgtcata ttgctttacg
     4741 tcgagagact ggtcaaagga tccggtgaga tcattgagcg cattgttacc caaaatcagc
     4801 atcgagaaat taacaagatc gcgacgctgg gcgacgggaa catgtatgcg agcacattgg
     4861 ataaggttgc caaaggcggt cgatagagct ctagtatttg aatagtgatt cctgctgctc
     4921 attctcacaa atgtctatga atgtcgtctg tccatcgctt gaaatcaaag aatgcgatac
     4981 atggcgtgtc ctgatgcagc gattccaatt gtaaatgcgt gcatgtgttc catgggcctg
     5041 cgttaaaatg gattctgcat gtgcagcaat aaatcaataa aaggatgaga catgcagcaa
     5101 ggaattcact gaaagctatg gaaggatatc gaataaagaa caatttggaa gctcatagaa
     5161 attggatgtg atggtccaaa atggatttcc gttttgcttt ccgcgcgacg ctctttttct
     5221 ttttacttgt cttctttcaa caaacccccc cttcttctac tttctcgttg aaaacgatac
     5281 aaggaagaaa gcgagagttc aaaaggtaat aaaagccctt gcttttgcag tttgctttta
     5341 cgtagagttt gttcgcgtca tcagccagag cgccctttta cctggcaacg cgacgctttc
     5401 aatttatttc tcgctgtgtg aaatactttt catgctgact atcccttgtc catccccatt
     5461 ccttccgtca tttgcttgct agctcgctcg cgaagattcc ctctcaatcc ttctccctat
     5521 ttggcaccct cctcccgaca ccgggcacta acctcgccgc agatattctc atcggcgtcg
     5581 tcgctattgg agttgtaggc ctacttgcaa acttcctctt tcgcagaaca tcaagcagca
     5641 ccaagcgtcg gcgtaataaa aaacgcacga agcaagaagc tactcatcgt caacaggagc
     5701 cgcttttaga cgagcgacat aaagacacta ccagtaacgg accaaagatc agcacaattt
     5761 aattaatggg taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     5821 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     5881 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     5941 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     6001 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     6061 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     6121 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     6181 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     6241 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     6301 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     6361 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6421 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     6481 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     6541 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     6601 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     6661 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     6721 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     6781 gggcaaagga aggaggagga ggaggaggaa gtaaaggaga agaacttttc actggagttg
     6841 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     6901 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     6961 aactacctgt tccatggcca acacttgtca ctactttcac ttatggtgtt caatgctttt
     7021 caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     7081 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     7141 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     7201 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     7261 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     7321 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     7381 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     7441 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     7501 gcatggatga actatacaaa taggcgcgcc acttctaaat aagcgaattt cttatgattt
     7561 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     7621 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     7681 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg gaattcgtaa
     7741 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7861 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7921 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcaat tcggcgttaa
     7981 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     8041 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     8101 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     8161 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     8221 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     8281 cgttgctgcc tgtgatccag atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     8341 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat
     8401 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     8461 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     8521 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     8581 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     8641 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     8701 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     8761 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     8821 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     8881 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     8941 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     9001 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     9061 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     9121 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat
     9181 gatcctgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10021 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    10081 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
    10141 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
    10201 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    10261 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
    10321 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
    10381 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
    10441 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
    10501 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
    10561 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
    10621 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
    10681 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
    10741 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
    10801 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
    10861 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
    10921 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
    10981 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
    11041 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    11101 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    11161 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    11221 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    11281 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    11341 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    11401 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    11461 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    11521 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    11581 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    11641 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    11701 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    11761 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    11821 tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt cctcgggctt gggggttcca
    11881 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
    11941 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    12001 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    12061 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    12121 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    12181 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    12241 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    12301 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    12361 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggac
//
