LOCUS       sequence-261542-       11989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (I52P)
FEATURES             Location/Qualifiers
     source          1..11989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(290..312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      898..2687
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2993..4918)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5353..5371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5439..5543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5544..5612,5613..6404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5762..5781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6575..7163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7064..7083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7780..7925
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7965..8990
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7965..8990
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(9011..9030)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9055..9103
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9756..10135
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10136..10339
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10290..10310
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10336..10360
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10442..10471
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(11519..11521,11522..11980)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(11561..11582)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11822..11841)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      241 gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      301 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc ggtcgactct
      421 agagccccgc cgccggacga actaaacctg actacggcat ctctgcccct tcttcgctgg
      481 tacgaggagc gcttttgttt tgtattcggg gcagtgcatg taatcccttc agttggttgg
      541 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg
      601 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt
      661 ggctttcatc ctggagcaga ctttgcatgc tgtggactgc aacacaacat tgcctttatg
      721 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc
      781 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa
      841 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac
      901 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag
      961 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag
     1021 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt
     1081 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc
     1141 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata
     1201 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga
     1261 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat
     1321 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata
     1381 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca
     1441 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta
     1501 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac
     1561 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc
     1621 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt
     1681 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg
     1741 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca
     1801 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg
     1861 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc
     1921 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg
     1981 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta
     2041 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     2101 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     2161 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     2221 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta
     2281 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     2341 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     2401 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     2461 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     2521 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc
     2581 atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc
     2641 gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgcat gctggttgct
     2701 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac
     2761 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac
     2821 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca
     2881 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc
     2941 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct
     3001 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct
     3061 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa
     3121 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct
     3181 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac
     3241 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg
     3301 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta
     3361 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
     3421 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt
     3481 tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt
     3541 tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat
     3601 ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt
     3661 cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg
     3721 gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc
     3781 tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc
     3841 tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc
     3901 ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc
     3961 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     4021 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg
     4081 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     4141 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg
     4201 cccctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4261 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc
     4321 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     4381 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     4441 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc
     4501 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     4561 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4621 ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa
     4681 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg
     4741 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc
     4801 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg
     4861 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga
     4921 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag
     4981 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca
     5041 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt
     5101 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
     5161 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
     5221 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
     5281 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
     5341 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5401 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5521 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5581 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5641 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5701 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5761 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     5821 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5881 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5941 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6001 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6061 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     6121 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6181 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6241 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6301 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6361 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6421 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6481 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6721 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7201 tttgctgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     7261 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     7321 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     7381 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     7441 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc
     7501 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     7561 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     7621 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     7681 gcgtcggccg ccatgccctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg
     7741 tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc
     7801 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     7861 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg
     7921 cttaacagcg tcaacagcgt gccgcagatc ccgggcaatg agatatgaaa aagcctgaac
     7981 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     8041 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     8101 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     8161 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     8221 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     8281 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     8341 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     8401 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     8461 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     8521 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     8581 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     8641 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     8701 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     8761 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     8821 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     8881 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     8941 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag gggagatggg
     9001 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     9061 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     9121 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     9181 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     9241 aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc
     9301 ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtctgg
     9361 tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca
     9421 tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa
     9481 aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct
     9541 tatcatcgca gatccgggca acgttgttgc attgctgcag gcgcagaact ggtaggtatg
     9601 gaagatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc
     9661 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     9721 ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta
     9781 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     9841 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9901 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9961 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
    10021 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    10081 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    10141 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
    10201 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    10261 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga
    10321 ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct gggtaccagc
    10381 tgctagcgcc accatgaaga ccatcatcgc cctgagctac atcttctgcc tggtgttcgc
    10441 cgagcagaaa ttgatcagcg aggaggacct gggcagccct ggaggcagtt caggaggcgg
    10501 atctcactcc atgaggtatt tcttcacatc cgtgtcccgg cccggccgcg gggagccccg
    10561 cttcatcgca gtgggctacg tggacgacac gcagttcgtg cggttcgaca gcgacgccgc
    10621 gagccagagg atggagccgc gggcgccgtg gccagagcag gagggtccgg agtattggga
    10681 cggggagaca cggaaagtga aggcccactc acagactcac cgagtggacc tggggaccct
    10741 gcgcggctac tacaaccaga gcgaggccgg ttctcacacc gtccagagga tgtatggctg
    10801 cgacgtgggg tcggactggc gcttcctccg cgggtaccac cagtacgcct acgacggcaa
    10861 ggattacatc gccctgaaag aggacctgcg ctcttggacc gcggcggaca tggcagctca
    10921 gaccaccaag cacaagtggg aggcggccca tgtggcggag cagttgagag cctacctgga
    10981 gggcacgtgc gtggagtggc tccgcagata cctggagaac gggaaggaga cgctgcagcg
    11041 tacggacgcc cccaaaacgc atatgactca ccacgctgtc tctgaccatg aagccaccct
    11101 gaggtgctgg gccctgagct tctaccctgc ggagatcaca ctgacctggc agcgggatgg
    11161 ggaggaccag acccaggaca cggagctcgt ggagaccagg cctgcagggg atggaacctt
    11221 ccagaagtgg gcggctgtgg tggtgccttc tggacaggag cagagataca cctgccatgt
    11281 gcagcatgag ggtttgccca agcccctcac cctgagatgg gagccgtctt cccagcccac
    11341 catccccatc gtgggcatca ttgctggcct ggttctcttt ggagctgtga tcactggagc
    11401 tgtggtcgct gctgtgatgt ggaggaggaa gagctcagat agaaaaggag ggagctactc
    11461 tcaggctgca agcagtgaca gtgcccaggg ctctgatgtg tctctcacag cttgtaaagt
    11521 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
    11581 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
    11641 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
    11701 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
    11761 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
    11821 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
    11881 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
    11941 ggagtacaac tacaacagcc acaacgtcta tctgaccgcc taactcgag
//
