LOCUS       sequence-261546-        3355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a fluorecent protein with an RNA binding peptide: 
            mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter 
            (J23106), ribozyme PlmJ and RBS BBa_B0034..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, 
            Simmel FC
  TITLE     Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in
            a Cell-Free Gene Expression System.
  JOURNAL   Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 
            10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
  PUBMED    29564885
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.8b00526"; journalName: "Nano Lett"; date: 
            "2018-04-11- 11"; volume: "18"; issue: "4"; pages: "2650-2657"
COMMENT     Sequence Label: pSB1C3_mTq-Tat (c-plasmid)
FEATURES             Location/Qualifiers
     source          1..3355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(109..697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(189..208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(880..974)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(995..1654)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1569..1588
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1816..1834
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1838..1881)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     RBS             2898..2909
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(3006..3040)
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     misc_feature    3191..3211
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3212..3269
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3293..3310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
       61 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      841 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
      901 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      961 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1021 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1081 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1141 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1201 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1261 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1321 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1381 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1441 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1501 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1561 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1621 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1681 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1741 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     1801 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     1861 gctttcgcta aggatgattt ctggaattcg agtaagccgc ttctagagca ctagatgtcc
     1921 tgaggtgcat actagtatgc acctcaggac atctagaagc ggcttactcg aattcgagta
     1981 agccgcttct agagcaccga ttgagaagag aaaagaaaac cgccgatcct gtccaccgca
     2041 ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa agtagtacca
     2101 ttaacgacga atgcgacgac ccttaccgcg ggtaccacga ggacgcggac cgctgctgcc
     2161 gccgccacca cgggtcttgt acagttcgtc catacccagc gtgatacccg ccgcggtaac
     2221 aaattccagc aggaccatgt ggtcacgttt ttcgttcgga tctttggaca gtttggattg
     2281 cgtgctcaag tagtgattat ctggcagcag gaccggacca tcgccaatcg gggtgttctg
     2341 ttgatagtga tcggccaatt gcacgccgcc atcttcaata ttgtgacgga tcttgaagtt
     2401 tgctttaatg ccgtttttct gtttatcagc ggtgatgtac acattgtcgc tgaagtaatt
     2461 atactccagt ttgtgaccca ggatgttacc gtcctcttta aagtcaatac ctttcagctc
     2521 aatacgattc accagcgtgt caccctcgaa cttgacttct gcgcgggtct tatagttacc
     2581 gtcgtctttg aaaaagatcg tacgttcctg aacgtagcct tccggcatcg cgctcttaaa
     2641 gaagtcatgc tgtttcatgt gatccggata gcgcgcgaaa cattgcacgc cccacgacag
     2701 agtcgtcacc agggtcggcc acggaaccgg cagcttaccg gtcgtgcaga taaacttcag
     2761 ggtcaattta ccgtaggtgg cgtcgccctc gccctcaccg ctaacgctaa acttgtgacc
     2821 gttgacatca ccatccagct caaccagaat cggcacgacg ccggttaaca gttcttcacc
     2881 cttgctaacc atgtctattt ctcctcttta ctgactttaa acaaaattat ttgtagaggt
     2941 cataagtttc gtcgcatttc agcgactcat cagtgggctt agcccagact tatgactgaa
     3001 gcttcgctag cactatacct aggactgagc tagccgtaaa gatcctgaat ctcgagattg
     3061 gtgccttgct tggattatga aatgccagcc tgatcataca acgttggacg aagtagcttt
     3121 accgtccaca cggtcggaac ttggtgtgcc gaatccaagt cgagataatc actagatgtc
     3181 ctgaggtgca tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac
     3241 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
     3301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaa
//
