LOCUS       sequence-261549-       10775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Foight GW, Wang Z, Wei CT, Jr Greisen P, Warner KM, 
            Cunningham-Bryant D, Park K, Brunette TJ, Sheffler W, Baker D, Maly 
            DJ
  TITLE     Multi-input chemical control of protein dimerization for programming
            graded cellular responses.
  JOURNAL   Nat Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8.
  PUBMED    31501561
REFERENCE   2  (bases 1 to 10775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8."
COMMENT     Sequence Label: GWF058
FEATURES             Location/Qualifiers
     source          1..10775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             450..494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    761..878
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             1253..1963
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1398..1416)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1666..1685
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1982..1999)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1988..2212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2223..2526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2527..2730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2681..2701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2835..2859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3795..3851
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4542..4571
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4572..4721
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4728..4748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5178..5549
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             5568..6137
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     LTR             6253..6486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6558..6679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6595..6614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6649..6668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6719..6854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6874..6893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6875..6893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6903..6920)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6903..6919)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6912..6934)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7061..7516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7148..7167)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7358..7379
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7542..7646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7647..7715,7716..8507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7865..7884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8678..9266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9167..9186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9420..9437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9554..9575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9590..9595,9596..9613,9614..9620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9628..9644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9633..9655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9652..9668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9652..9668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9687..9707
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9689..9707
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9735..9961
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9962..10142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10189..10314
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
       61 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
      121 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
      181 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
      241 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
      301 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
      361 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
      421 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
      481 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
      541 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
      601 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
      661 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      721 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
      781 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      841 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      901 gagatccagt ttggatcgat aagcttgata tcgaattcgg agcactgtcc tccgaacgtc
      961 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
     1021 aacggagcat gtcctccgaa cgtcggagca ctgtcctccg aacgactagt taggcgtgta
     1081 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     1141 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ctcgacattc
     1201 gttggtgccc caactggggt aacctttgag ttctctcagt tgggggacca ccatggtgag
     1261 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     1321 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1381 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1441 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     1501 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1561 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     1621 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1681 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1741 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1801 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1861 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1921 gggccgccac tccaccggcg gcatggacga gctgtacaag taaatcgcgc gcgactcgag
     1981 ccctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2041 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2101 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2161 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggatctaaac
     2221 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2281 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2341 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2401 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2461 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2521 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2581 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2641 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2701 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2761 cgccatccac gctgttttga cctccataga agacaccgac tctagtccag tgtggtggaa
     2821 ttctgcagat atcaacaagt ttgtacaaaa aagttggcac catgaagcta ctgtcttcta
     2881 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
     2941 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
     3001 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
     3061 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     3121 aggatataaa agcattgttg ggtacccctg cagctgcgtc gactctagag ggaggcggct
     3181 ccgcaggctc tggcggaaag aagaagggaa gtgtagtcat cgtgggtagg attaatttga
     3241 gtggggatac ggcatacgca caacaaactc ggggggaaga aggatgtcaa gagacctctc
     3301 aaacgggtag ggataagaat caggtggaag gagaggttca aatcgtttcc acggcgacgc
     3361 aaacatttct tgcgacctcc attaatggag tgctttggac agtatatcat ggagcgggga
     3421 ccagaactat agcctccccg aaagggccag tcacgcaaat gtatacaaac gtggataaag
     3481 atctcgtagg gtggcaagca cctcaaggct cacgaagtct gactccatgt acgtgtggat
     3541 cctccgatct gtatctcgtc acacgccatg cagatgtaat cccggtaagg cgaagaggag
     3601 attcaagagg aagtttgctg tccccaagac ctatttccta cctcaaggga tcagcaggag
     3661 gacccttgct gtgtcccgcc gggcacgcag tgggtatatt ccgcgctgct gtcagcacaa
     3721 ggggcgtagc taaggcggtc gattttattc ctgttgagtc acttgaaacc acgatgcgat
     3781 ctccaggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     3841 accctggacc tatgtcttcc gacgaagaag aagcgcgcga gctgatcgag cgtgcgaagg
     3901 aggctgcgga acgcgcgcaa gaagctgctg agcgtaccgg tgatccgcgc gtgcgtgaac
     3961 tcgctcgtga gctgaaacgc ctggctcagg aagctgctga agaagtgaag cgtgacccgt
     4021 cttcctccga tgtgaacgaa gcgctcaaac tcatcgtgga agctatcgag gcggctgtgg
     4081 acgcgctcga agcggctgaa cgcaccggcg acccggaggt tcgtgagctg gcgcgtgagc
     4141 tcgtgcgcct cgcggttgag gctgcggagg aggtgcaacg taacccatcc tcctctgacg
     4201 tgaatgaggc tctccacagc atagtttacg ctattgaagc tgccattttt gcattggaag
     4261 ctgccgaaag gactggtgac ccggaagtga gagaactcgc ccgagagctt gtaagactgg
     4321 cggttgaggc ggctgaagaa gtccagcgaa atccgtccag ccggaatgtc gagcacgcac
     4381 tgatgaggat tgtgttggcc atttacctgg cagaagaaaa tctccgcgaa gctgaggagt
     4441 ctggcgatcc ggaaaaacgt gaaaaggctc gcgaacgtgt tcgtgaggcg gtggaacgtg
     4501 cggaagaggt tcagcgtgat ccatccggtt ggctgaatca tgaacaaaaa cttatttccg
     4561 aagaggatct ggatgcttta gacgattttg acttagatat gcttggttca gacgcgttag
     4621 acgacttcga cctagacatg ttaggctcag atgcattgga cgacttcgat ttagatatgt
     4681 tgggctccga tgccctagat gactttgatc tagatatgct aggtagtccc aaaaagaaga
     4741 ggaaagtggg atcccagtat ctgcccgaca cagatgatag acaccgaatc gaagagaaac
     4801 gcaagcgaac gtatgaaacc ttcaaatcga tcatgaagaa atcgcccttc tcgggtccga
     4861 ccgatcccag gcccccaccg agaaggattg cggtcccgtc ccgctcgtcg gccagcgtgc
     4921 cgaagcctgc gccgcagccc taccccttca cgtcgagcct gagcacaatc aattatgacg
     4981 agttcccgac gatggtgttc ccctcgggac aaatctcaca agcctcggcg ctcgcaccag
     5041 cgcctcccca agtccttccg caagcgcctg ccccagcgcc tgcaccggca atggtgtccg
     5101 ccctcgcaca ggcccctgcg cccgtccccg tgctcgcgcc tggaccgccc caggcggtcg
     5161 ctccaccggc tccgaagccg acgcaggccg gagagggaac actctccgaa gcacttcttc
     5221 aactccagtt tgatgacgag gatcttggag cactccttgg aaactcgaca gaccctgcgg
     5281 tgtttaccga cctcgcgtca gtagataact ccgaatttca gcagcttttg aaccagggta
     5341 tcccggtcgc gccacataca acggagccca tgttgatgga ataccccgaa gcaatcacga
     5401 gacttgtgac gggagcgcag cggcctcccg atcccgcacc cgcacctttg ggggcacctg
     5461 gcctccctaa cggacttttg agcggcgacg aggatttctc ctccatcgcc gatatggatt
     5521 tctcagcctt gctgtcacag atttccagcg gctctggcag cggcagccgg gattccaggg
     5581 aagggatgtt tttgccgaag cctgaggccg gctccgctat tagtgacgtg tttgagggcc
     5641 gcgaggtgtg ccagccaaaa cgaatccggc catttcatcc tccaggaagt ccatgggcca
     5701 accgcccact ccccgccagc ctcgcaccaa caccaaccgg tccagtacat gagccagtcg
     5761 ggtcactgac cccggcacca gtccctcagc cactggatcc agcgcccgca gtgactcccg
     5821 aggccagtca cctgttggag gatcccgatg aagagacgag ccaggctgtc aaagcccttc
     5881 gggagatggc cgatactgtg attccccaga aggaagaggc tgcaatctgt ggccaaatgg
     5941 acctttccca tccgccccca aggggccatc tggatgagct gacaaccaca cttgagtcca
     6001 tgaccgagga tctgaacctg gactcacccc tgaccccgga attgaacgag attctggata
     6061 ccttcctgaa cgacgagtgc ctcttgcatg ccatgcatat cagcacagga ctgtccatct
     6121 tcgacacatc tctgttttga taacctggtg catgacccgc aagtaagcac aattcgagct
     6181 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6241 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     6301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6481 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6541 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6661 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6721 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6841 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6901 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6961 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7021 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7501 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8341 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9361 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9421 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9661 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9721 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     9781 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     9841 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     9901 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     9961 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10141 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    10201 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    10261 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10321 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    10381 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    10441 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    10501 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    10561 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    10621 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    10681 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    10741 attaggagta gcacccacca aggcaaagag aagag
//
