LOCUS       sequence-26155-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pCDNA3-myc3-FBW5
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atggagcaaa agctcatctc agaggaagat ctggaattag
      121 agcaaaagct catctcagag gaagatctgg aattagagca aaagctcatc tcagaggaag
      181 atctggaatt cacgatggac gagggcggca cgcccctgct ccccgacagc ctggtctacc
      241 agatcttcct gagcctgggc ccggccgacg tgctggccgc cgggctggtg tgccgccaat
      301 ggcaggccgt gtcgcgggac gagttcctgt ggagggagca gttctaccgc tactaccagg
      361 tggcccgcga cgtgccccga cacccagcgg ccatgtcctg gtacgaggag ttccagcggc
      421 tgtatgacac ggtgccctgc gtggaggtgc agacgctgcg ggaacacaca gaccaggtcc
      481 tgcacctcag cttctcccat tccgggtacc agttcgcgtc ctgctccaag gactgcactg
      541 tgaagatctg gagcaacgac ctgaccatct cgctgctgca cagcgcggac atgcggccct
      601 acaactggag ctacacccag ttctcccagt tcaacaagga cgactcgcta ctgctggcct
      661 cgggggtgtt cctggggccg cacaactcct catccggcga gattgctgtc atcagcctag
      721 actccttcgc gctgctgtcc cgcgtgcgga acaagcccta tgacgtgttt ggctgttggc
      781 tcaccgagac cagcctcatc tcggggaacc tgcaccgcat c
//
