LOCUS       sequence-261555-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Foight GW, Wang Z, Wei CT, Jr Greisen P, Warner KM, 
            Cunningham-Bryant D, Park K, Brunette TJ, Sheffler W, Baker D, Maly 
            DJ
  TITLE     Multi-input chemical control of protein dimerization for programming
            graded cellular responses.
  JOURNAL   Nat Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8.
  PUBMED    31501561
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8."
COMMENT     Sequence Label: GWF243
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        504..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     658..678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     704..728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      790..799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             799..828
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(1527..2100,2101..2103,2104..2112)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1694..1711)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1921..1940
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2128..2130,2131..2133,2134..2844)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2128..2130,2131..2133,2134..2844)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2173..2194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2434..2453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2781..2802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2857..2886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4276..4332
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4333..5028
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             5041..5070
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        6621..6832
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6845..7113
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7140..7739
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7140..7159)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7636..7656
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7799..7819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7861..7982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7898..7917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7952..7971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8745..8779
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9270..9286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9270..9286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9283..9305)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9294..9310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9318..9324,9325..9342,9343..9348))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9363..9384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9501..9518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9672..10260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9752..9771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10431..11222,11223..11291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11054..11073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11292..11396)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11422..11877)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11559..11580)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11771..11790
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12004..12026
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12018..12035
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12019..12035
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   12165..12227
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
       61 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      121 ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg
      181 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
      241 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
      301 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
      361 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc
      421 actagtatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      661 aaatgggcgg taggcgtgta cggtgggagg tttatataag cagagctcgt ttagtgaacc
      721 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga ttctagagct
      781 agcgaattcg ccaccatgga gcaaaagctg attagcgaag aagacctggg tggcaagaag
      841 aagggaagtg tagtcatcgt gggtaggatt aatttgagtg gggatacggc atacgcacaa
      901 caaactcggg gggaagaagg atgtcaagag acctctcaaa cgggtaggga taagaatcag
      961 gtggaaggag aggttcaaat cgtttccacg gcgacgcaaa catttcttgc gacctccatt
     1021 aatggagtgc tttggacagt atatcatgga gcggggacca gaactatagc ctccccgaaa
     1081 gggccagtca cgcaaatgta tacaaacgtg gataaagatc tcgtagggtg gcaagcacct
     1141 caaggctcac gaagtctgac tccatgtacg tgtggatcct ccgatctgta tctcgtcaca
     1201 cgccatgcag atgtaatccc ggtaaggcga agaggagatt caagaggaag tttgctgtcc
     1261 ccaagaccta tttcctacct caagggatca gcaggaggac ccttgctgtg tcccgccggg
     1321 cacgcagtgg gtatattccg cgctgctgtc agcacaaggg gcgtagctaa ggcggtcgat
     1381 tttattcctg ttgagtcact tgaaaccacg atgcgatctc catccgcagg agggagtgct
     1441 ggaggagaga aaatgtccaa ggatggtaag aaaaagaaga agaagtcaaa aaccaagtgt
     1501 gttatcatgt gaatttaaat aattcgcccc tctccctccc ccccccctaa cgttactggc
     1561 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     1621 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     1681 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     1741 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     1801 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     1861 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     1921 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     1981 atgggatctg atctggggcc tcggtgcaca tgctttacgt gtgtttagtc gaggttaaaa
     2041 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     2101 atggccacaa ccggatccgg agctaccatg gtgagcaagg gcgaggagct gttcaccggg
     2161 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2221 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2281 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2341 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2401 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2461 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2521 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2581 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2641 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2701 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2761 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2821 ctcggcatgg acgagctgta caagagtggc agtggcgagc aaaaattgat ctcagaggag
     2881 gatctgggtt ccgggtcttc cgacgaagaa gaagcgcgcg agctgatcga gcgtgcgaag
     2941 gaggctgcgg aacgcgcgca agaagctgct gagcgtaccg gtgatccgcg cgtgcgtgaa
     3001 ctcgctcgtg agctgaaacg cctggctcag gaagctgctg aagaagtgaa gcgtgacccg
     3061 tcttcctccg atgtgaacga agcgctcaaa ctcatcgtgg aagctatcga ggcggctgtg
     3121 gacgcgctcg aagcggctga acgcaccggc gacccggagg ttcgtgagct ggcgcgtgag
     3181 ctcgtgcgcc tcgcggttga ggctgcggag gaggtgcaac gtaacccatc ctcctctgac
     3241 gtgaatgagg ctctccacag catagtttac gctattgaag ctgccatttt tgcattggaa
     3301 gctgccgaaa ggactggtga cccggaagtg agagaactcg cccgagagct tgtaagactg
     3361 gcggttgagg cggctgaaga agtccagcga aatccgtcca gccggaatgt cgagcacgca
     3421 ctgatgagga ttgtgttggc catttacctg gcagaagaaa atctccgcga agctgaggag
     3481 tctggcgatc cggaaaaacg tgaaaaggct cgcgaacgtg ttcgtgaggc ggtggaacgt
     3541 gcggaagagg ttcagcgtga tccatccggt tggctgaatc atagtgctgg tggtagtgca
     3601 ggaggctcgg ccggtggaag tgcaggaggg agtggcgcta gcagacaact ctcggatgca
     3661 gataagctgc gcaaggtgat ctgcgagctc ctggagacgg agcgcaccta cgtgaaggat
     3721 ttaaactgtc ttatggagag atacctaaag cctcttcaaa aagaaacttt tctcacccag
     3781 gatgagcttg acgtgctttt tggaaattta acggaaatgg tagagtttca agtagaattc
     3841 cttaaaactc tagaagatgg agtgagactg gtacctgatt tggaaaagct tgagaaggtt
     3901 gatcaattta agaaagtgct gttctctctg gggggatcat tcctgtatta tgctgaccgc
     3961 ttcaagctct acagtgcctt ctgcgccagc cacacaaaag ttcccaaggt cctggtgaaa
     4021 gccaagacag acacggcttt caaggcattc ttggatgccc agaacccgaa gcagcagcac
     4081 tcatccacgc tggagtcgta cctcatcaag cccatccaga ggatcctcaa gtacccactt
     4141 ctgctcaggg agctgttcgc cctgaccgat gcggagagcg aggagcacta ccacctggac
     4201 gtggccatca agaccatgaa caaggttgcc agtcacatca atgagatgca gaaaatccat
     4261 gaagagggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     4321 aaccctggac ctagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     4381 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     4441 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     4501 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     4561 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     4621 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     4681 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     4741 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     4801 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
     4861 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     4921 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     4981 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatag tggcagtggc
     5041 gagcaaaaat tgatctcaga ggaggatctg ggttccggga cgggatctgg tactggttct
     5101 ggtaccggga caacgtccgg aacagggaca gggggctcca ctgggatgga tatcgagaag
     5161 ctttgcaaaa aagctgaaga agaggcaaag gaggcgcaag agaaagctga tgagttgcgg
     5221 caacggcacc ccgacagcca ggctgctgag gatgctgagg acttggcaaa tctggcggtg
     5281 gccgctgtct tgacggcttg cttgttggcc caagaacatc caaacgctga cattgcaaag
     5341 ctttgcatta aggccgcgtc cgaagcggct gaagcagctt ctaaggcagc cgagctggcg
     5401 caacggcatc ctgactccca agcggcgagg gacgctataa aactcgcatc acaagcagcc
     5461 agagctgtaa tcctcgctat tatgctcgca gccgaaaacc caaacgctga tattgccaaa
     5521 ctgtgtataa aggcggctag tgaagccgca gaagctgcaa gcaaagcagc agaacttgcc
     5581 caacgccacc ccgactccca ggcggctaga gatgctataa aacttgcttc tcaggctgcg
     5641 gaggcggtag aacgagcgat ctggctggca gcagagaacc ccaacgctga cattgccaaa
     5701 aagtgtatca aggcggcttc cgaagccgcc gaggaggcca gcaaagcggc ggaagaggca
     5761 cagagacatc cggactcaca gaaagcacgc gacgagatta aagaggctag tcaaaaagca
     5821 gaggaggtaa aagaacgctg taagtcctcc gcaggtggca gtgcaggtgg cagcgccggc
     5881 ggtagtgccg gagggagtgc tggcacccca cccaactggc agcagcttgt tagtcgagaa
     5941 gtgttactgg gactaaaacc ttgtgaaatc aaaagacagg aagtgattaa tgaattgttc
     6001 tacactgaaa gagctcatgt tcgaacactg aaggttcttg atcaagtgtt ctatcagcga
     6061 gtatccagag aaggaattct gtcaccctca gagctacgga aaattttttc aaacttggaa
     6121 gatattcttc aacttcatat tggattgaat gaacaaatga aggctgttcg aaagagaaat
     6181 gagacctctg ttatcgatca gattggggaa gatttgctga catggttcag cggaccagga
     6241 gaggagaaat tgaaacatgc tgctgctacc ttttgcagta accaaccttt cgccctggaa
     6301 atgatcaaat ctcgtcagaa aaaggattct cgatttcaga cttttgtgca agatgctgaa
     6361 agtaatccac tgtgtcgtcg tcttcaactg aaggatatta ttcccactca aatgcaaagg
     6421 cttactaagt acccacttct gttggataat attgccaaat acacagaatg gccaacagaa
     6481 agggagaagg tgaagaaagc tgcagatcac tgtcgtcaga tcttaaatta tgtaaatcag
     6541 gctgtcaagg aggcagaaaa caagcagcgc tgaggatccg cggccgcgaa ggatctgcga
     6601 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     6661 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     6721 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     6781 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     6841 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6901 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6961 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     7021 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     7081 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     7141 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     7201 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     7261 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7321 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7381 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7441 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7501 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7561 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7621 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7681 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     7741 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gcgttaacta
     7861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7981 tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct catctggtct
     8041 cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg
     8101 ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga
     8161 agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt
     8221 agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     8281 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     8341 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     8401 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     8461 ggctgtccct gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     8521 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     8581 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     8641 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     8701 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     8761 gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt
     8821 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
     8881 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
     8941 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
     9001 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
     9061 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     9121 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     9181 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     9241 acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9301 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9361 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9481 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11401 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
    11461 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
    11521 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    11581 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    11641 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    11701 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    11761 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    11821 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
    11881 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    11941 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    12001 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
    12061 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
    12121 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
    12181 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatg
//
