LOCUS       sequence-26156-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-HA2-CUL4A
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atgtacccat acgatgttcc tgactatgcg ggctatccct
      121 atgacgtccc ggactatgca agcttggtac cgttaacatg gatcccccgg gctgcaggaa
      181 ttcggcacga gccagccatg gcggacgagg ccccgcggaa gggcagcttc tcggcgctcg
      241 tgggccgcac caacggcctc accaagcccg cggccctggc cgccgcgccc gccaagccgg
      301 ggggcgcggg cggctccaag aagctggtca tcaagaactt ccgagacaga cctcggctgc
      361 ccgacaacta cacgcaggac acgtggcgga agctgcacga ggcggtgcgg gccgtgcaga
      421 gcagcacctc catcaggtac aacctcgagg agctctacca ggctgtggaa aatctctgtt
      481 ctcacaaagt ctccccaatg ctctacaagc aactgcgtca ggcctgtgaa gaccacgtcc
      541 aggcacagat ccttccgttt agagaagact cactagatag tgttttattt ttaaagaaga
      601 ttaacacgtg ctggcaggac cactgcagac aaatgatcat gatcagaagc atcttcctgt
      661 tcttggaccg cacctatgtg ctgcagaact ccacgctgcc ctccatctgg gatatgggat
      721 tagaactgtt tagaacccat attattagtg ataaaatggt tcagagtaaa accattgatg
      781 gaatcctact gctgatcgag cgcgagagga gcggcgaggc cgtgga
//
