LOCUS       sequence-261562-       12415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rieske FeS overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Ermakova M, Lopez-Calcagno PE, Raines CA, Furbank RT, von Caemmerer 
            S
  TITLE     Overexpression of the Rieske FeS protein of the Cytochrome b 6 f 
            complex increases C4 photosynthesis in Setaria viridis.
  JOURNAL   Commun Biol. 2019 Aug 16;2:314. doi: 10.1038/s42003-019-0561-9. 
            eCollection 2019.
  PUBMED    31453378
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-08-16"; volume: "2:314. doi"; pages: " 
            10.1038/s42003-019-0561-9. eCollection 2019"
COMMENT     Sequence Label: p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise
FEATURES             Location/Qualifiers
     source          1..12415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             351..1424
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1490..1684
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1746..1850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1851..2645
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2816..3404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3305..3324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3540..3564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3655..3907)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          4607..4796
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             complement(5676..5693)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(6561..6813)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(7493..9489)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     terminator      complement(9516..9768)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(9781..10494,10495..10497,10498..10500))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9826..9847)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10175..10194
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10434..10455
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(10591..10936)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10628..10648)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     RBS             11339..11347
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    11404..11428
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11708..12337
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
       61 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
      121 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
      181 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
      241 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
      301 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
      361 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
      421 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
      481 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
      541 cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
      601 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
      661 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
      721 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
      781 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
      841 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
      901 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
      961 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     1021 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     1081 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     1141 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     1201 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     1261 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     1321 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     1381 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     1441 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct gtggatagca
     1501 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     1561 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     1621 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     1681 cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca tcaaggctcc
     1741 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
     1861 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     1921 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     1981 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     2041 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     2101 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     2161 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     2221 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     2281 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
     2341 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     2401 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     2461 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     2521 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     2581 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     2641 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3421 cctttttacg gttcctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg
     3481 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     3541 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     3601 acgtttttaa tgtactgggg ttgaacactc tgtgccgaat tcggatccag cgtcgatcta
     3661 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     3721 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     3781 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     3841 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     3901 aacgatctgc ttgacaagcc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     3961 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     4021 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     4081 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     4141 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     4201 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     4261 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     4321 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgagatgc cggacttcgg
     4381 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     4441 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     4501 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     4561 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agttatcatc
     4621 atcatcatag acacacgaaa taaagtaatc agattatcag ttaaagctat gtaatattta
     4681 caccataacc aatcaattaa aaaatagatc agtttaaaga aagatcaaag ctcaaaaaaa
     4741 taaaaagaga aaagggtcct aaccaagaaa atgaaggaga aaaactagaa atttacctgc
     4801 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg ggcacggcgg
     4861 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     4921 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     4981 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     5041 tcgccctccg agagctgcat caggtcggac acgctgtcga acttttcgat cagaaacttc
     5101 tcgacagacg tcgcggtgag ttcaggcttt ttcattcttc tacctacaaa aaagctccgc
     5161 acgaggctgc atttgtcaca aatcatgaaa agaaaaacta ccgatgaaca atgctgaggg
     5221 attcaaattc tacccacaaa aagaagaaag aaagatctag cacatctaag cctgacgaag
     5281 cagcagaaat atataaaaat ataaaccata gtgccctttt cccctcttcc tgatcttgtt
     5341 tagcatggcg gaaattttaa accccccatc atctccccca acaacggcgg atcgcagatc
     5401 tacatccgag agccccattc cccgcgagat ccgggccgga tccacgccgg cgagagcccc
     5461 agccgcgaga tcccgcccct cccgcgcacc gatctgggcg cgcacgaagc cgcctctcgc
     5521 ccacccaaac taccaaggcc aaagatcgac accgacacgg aaaaaaaaaa cggagaaaga
     5581 aagaggagag gggcggggtg gttaccggcg cggcggcggc ggagggggag gggggaggag
     5641 ctcgtcgtcc ggcagcgagg ggggaggagg tggaggtggt ggtggtggtg gtggtagggt
     5701 tggggggatg ggaggagagg ggggggtatg tatatagtgg cgatgggggg cgtttctttg
     5761 gaagcggagg gagggccggc ctcgtcgctg gctcgcgatc ctcctcgcgt ttccggcccc
     5821 cacgacccgg acccacctgc tgttttttct ttttcttttt tttctttctt tttttttttt
     5881 tggctgcgag tcgtgcggtg cgtgcggaca actcacggtg atagtggggg ggtgtggaga
     5941 ctattgtcca gttggctgga ctggggtggg ttgggttggg ttgggttggg ctgggcttgc
     6001 tatggatcgt ggatagcact ttgggcttta ggaactttag gggttgtttt tgtaaatgtt
     6061 ttgagtctaa gtttatcttt tatttttact agaaaaaata cccatgcgct gcaacggggg
     6121 aaagctattt taatcttatt attgttcatt gtgagaattc gcctgaatat atatttttct
     6181 caaaaattat gtcaaattag catataggtt tttttaaaga tatttcttat acaaatccct
     6241 ctgtatttac aaaagcaaac gaacttaaaa cccgactcaa atacagatat gcatttccaa
     6301 aagcgaataa acttaaaaac caattcatac aaaaatgacg tatcaaagta ccgacaaaaa
     6361 catcctcaat ttttataata gtagaaaaga gtaaatttca ctttgggcca ccttttatta
     6421 ccgatatttt actttatacc accttttaac tgatgttttc acttttgacc aggtaatctt
     6481 acctttgttt tattttggac tatcccgact ctcttctcaa gcatatgaat gaccctccgc
     6541 aagaattcaa gcttagcgtc gatctagtaa catagatgac accgcgcgcg ataatttatc
     6601 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     6661 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     6721 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     6781 taagaaactt tattgccaaa tgtttgaacg atctgcttga caagcttatt tccaccatgg
     6841 gttctcgccg gtcctgaagt cggtttcgac ccacgggacg aacacgacct tgccatcatc
     6901 aacgtcagca tgaaccaggg ccagcgacag gggagcaggt ccacggacaa ccttgccctg
     6961 gttgttgtac tgggatccat ggcaagggca aaggaacttg ttctcggcgg tgttaaacgg
     7021 cacgacgcag ccaaggtggg tgcagaccgc gttgatcccg taggtggcga gggtcttgtc
     7081 ggcctccacg acgaggtacg tcggatcacc cttgagtccc tgtgcgagcg tgcggtcgtt
     7141 ggggccgtgc gtcttgagcc attcctcgac gaggatgtcg ttgccgagct tgtccttggc
     7201 gacggtcccg ccggtgttgc tcccggagcc ggcggggacg aggaaggagc cgtaggggac
     7261 gagcatgccg aaggtgggga gcgagatggc gcccagcagg aggaggttca tcagctccct
     7321 cttactcatg tccgggacgc ggtcggcgga gatgctgccg gccatgcacg tgatgttcct
     7381 cgggatgcgc tcccgggcga agcccaggcc cctgaccggc ttgcccagcg aaggaagggg
     7441 ccggcagagc cgagtggggt tggaggcggt ggagagcgcg gtggaagcca ttctgcagaa
     7501 gtaacaccaa acaacagggt gagcatcgac aaaagaaaca gtaccaagca aataaatagc
     7561 gtatgaaggc agggctaaaa aaatccacat atagctgctg catatgccat catccaagta
     7621 tatcaagatc aaaataatta taaaacatac ttgtttatta taatagatag gtactcaagg
     7681 ttagagcata tgaatagatg ctgcatatgc catcatgtat atgcatcagt aaaacccaca
     7741 tcaacatgta tacctatcct agatcgatat ttccatccat cttaaactcg taactatgaa
     7801 gatgtatgac acacacatac agttccaaaa ttaataaata caccaggtag tttgaaacag
     7861 tattctactc cgatctagaa cgaatgaacg accgcccaac cacaccacat catcacaacc
     7921 aagcgaacaa aaagcatctc tgtatatgca tcagtaaaac ccgcatcaac atgtatacct
     7981 atcctagatc gatatttcca tccatcatct tcaattcgta actatgaata tgtatggcac
     8041 acacatacag atccaaaatt aataaatcca ccaggtagtt tgaaacagaa ttaattctac
     8101 tccgatctag aacgaccgcc caaccagacc acatcatcac aaccaagaca aaaaaaagca
     8161 tgaaaagatg acccgacaaa caagtgcacg gcatatattg aaataaagga aaagggcaaa
     8221 ccaaacccta tgcaacgaaa caaaaaaaat catgaaatcg atcccgtctg cggaacggct
     8281 agagccatcc caggattccc caaagagaaa cactggcaag ttagcaatca gaacgtgtct
     8341 gacgtacagg tcgcatccgt gtacgaacgc tagcagcacg gatctaacac aaacacggat
     8401 ctaacacaaa catgaacaga agtagaacta ccgggcccta accatggacc ggaacgccga
     8461 tctagagaag gtagagaggg gggggggggg aggacgagcg gcgtaccttg aagcggaggt
     8521 gccgacgggt ggatttgggg gagatctggt tgtgtgtgtg tgcgctccga acaacacgag
     8581 gttggggaaa gagggtgtgg agggggtgtc tatttattac ggcgggcgag gaagggaaag
     8641 cgaaggagcg gtgggaaagg aatcccccgt agctgccggt gccgtgagag gaggaggagg
     8701 ccgcctgccg tgccggctca cgtctgccgc tccgccacgc aatttctgga tgccgacagc
     8761 ggagcaagtc caacggtgga gcggaactct cgagaggggt ccagaggcag cgacagagat
     8821 gccgtgccgt ctgcttcgct tggcccgacg cgacgctgct ggttcgctgg ttggtgtccg
     8881 ttagactcgt cgacggcgtt taacaggctg gcattatcta ctcgaaacaa gaaaaatgtt
     8941 tccttagttt ttttaatttc ttaaagggta tttgtttaat ttttagtcac tttattttat
     9001 tctattttat atctaaatta ttaaataaaa aaactaaaat agagttttag ttttcttaat
     9061 ttagaggcta aaatagaata aaatagatgt actaaaaaaa ttagtctata aaaaccatta
     9121 accctaaacc ctaaatggat gtactaataa aatggatgaa gtattatata ggtgaagcta
     9181 tttgcaaaaa aaaaggagaa cacatgcaca ctaaaaagat aaaactgtag agtcctgttg
     9241 tcaaaatact caattgtcct ttagaccatg tctaactgtt catttatatg attctctaaa
     9301 acactgatat tattgtagta ctatagatta tattattcgt agagtaaagt ttaaatatat
     9361 gtataaagat agataaactg cacttcaaac aagtgtgaca aaaaaaatat gtggtaattt
     9421 tttataactt agacatgcaa tgctcattat ctctagagag gggcacgacc gggtcacgct
     9481 gcactgcagc tccactagaa ttcgagctca gcgtcgatct agtaacatag atgacaccgc
     9541 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
     9601 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
     9661 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
     9721 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatctg cttgacaagc
     9781 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     9841 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
     9901 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     9961 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
    10021 gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atgtagttgt actccagctt
    10081 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
    10141 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
    10201 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
    10261 cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg tggtcacgag
    10321 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
    10381 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
    10441 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
    10501 tgtatcgata attgtaaatg taattgtaat gttgtttgtt gtttgttgtt gttggtaatt
    10561 gttgtaaaaa tgagctctta tactcgagcg tgtcctctcc aaatgaaatg aacttcctta
    10621 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    10681 gatgtcacat caatccactt gctttgtaga cgtggttgga acctcttctt tttccacgat
    10741 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagagagat cttgaatgat
    10801 agcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt ctactgtcct
    10861 ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg aaattatcct
    10921 ttgttgaaaa gtctcaatag ccctttgatc ttctgagact gtatctttga catttttgga
    10981 gtagaccaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgtagacgt
    11041 ggttggaacc tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    11101 actgtcggca gagagatctt gaatgatagc ctttccttta tcgcaatgat ggcatttgta
    11161 ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
    11221 tccgaggagg tttcccgaaa ttatcctttg ttgaaaagtc tcaatagccc tttgatcttc
    11281 tgagactgta tctttgacat ttttggagta gaccagagtg tcgtgctcca ccatgttgac
    11341 ctccttacga ggatgcacat gtgaccgagg gacacgaagt gatccgttta aactatcagt
    11401 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
    11461 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg ccagccgtgc
    11521 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
    11581 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
    11641 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
    11701 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
    11761 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
    11821 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
    11881 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
    11941 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
    12001 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
    12061 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
    12121 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
    12181 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
    12241 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
    12301 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
    12361 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caagg
//
