LOCUS       sequence-261569-       12046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-TM-VC
FEATURES             Location/Qualifiers
     source          1..12046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1405..1656
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1593..1614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1679..1813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1703..1722)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1757..1776
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1955..1977)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2563..4352
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4658..6583)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7018..7036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7104..7208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7209..7277,7278..8069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7427..7446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8240..8828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8729..8748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9445..9590
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9630..10655
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9630..10655
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10676..10695)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10720..10768
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11421..11800
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11801..12004
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11955..11975
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12001..12025
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccaagcccc acccagcaga ggggcagtgg cgggcagtgg acgtggtcct agactgtttc
      181 ctggtgaagg acggtgcgca ccgtggagct ctcgccagca gtgaggacag ggcaagggcc
      241 tcccttgtgc tgaagcaggt gccagtgctg gacgatggct ccctggagga cttcaccgat
      301 ttccaagggg gcacactggc ccaagatgac ccacctatta tctttgaggc ctcagtggac
      361 ctggtccaga ttccccaggc cgaggccttg ctccatgctg actgcagtgg gaaggaggtg
      421 acctgtgaga tctcccgcta ctttctccag atgacagaga ccactgttaa gacagcagct
      481 tggttcatgg ccaacgtgca ggtctctgga gggggaccta gcatctcctt ggtgatgaag
      541 actcccaggg tcgccaagaa tgaggtgctc tggcacccaa cgctgaactt gccactgagc
      601 ccccagggga ctgtgcgaac tgcagtggag ttccaggtga tgacacagac ccaatccctg
      661 agcttcctgc tggggtcctc agcctccttg gactgtggct tctccatggc accgggcttg
      721 gacctcatca gtgtggagtg gcgactgcag cacaagggca ggggtcagtt ggtgtacagc
      781 tggaccgcag ggcaggggca ggctgtgcgg aagggcgcta ccctggagcc tgcacaactg
      841 ggcatggcca gggatgcctc cctcaccctg cccggcctca ctatacagga cgaggggacc
      901 tacatttgcc agatcaccac ctctctgtac cgagctcagc agatcatcca gctcaacatc
      961 caagcttccc ctaaagtacg actgagcttg gcaaacgaag ctctgctgcc caccctcatc
     1021 tgcgacattg ctggctatta ccctctggat gtggtggtga cgtggacccg agaggagctg
     1081 ggtggatccc cagcccaagt ctctggtgcc tccttctcca gcctcaggca aagcgtggca
     1141 ggcacctaca gcatctcctc ctctctcacc gcagaacctg gctctgcagg tgccacttac
     1201 acctgccagg tcacacacat ctctctggag gagccccttg gggccagcac ccaggttgtc
     1261 ccaccagagc ggagatctgg agccggaagt gctatcccca tcatgggtat cgttgctggc
     1321 ctggttgtcc ttgcagctgt agtcactgga gctgcggtcg ctgctgtgct gtggagaaag
     1381 aagagctcag atggaggctc tggagacaag cagaagaacg gcatcaaggc caacttcaag
     1441 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     1501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag
     1561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1621 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcgaggccgg caaggccgga
     1681 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1741 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1801 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1861 ggggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     1921 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1981 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2041 cgggtgttgg cgggtgtcgg ggcgcagcca tgaccggtcg actctagagc cccgccgccg
     2101 gacgaactaa acctgactac ggcatctctg ccccttcttc gctggtacga ggagcgcttt
     2161 tgttttgtat tcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     2221 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     2281 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     2341 gcagactttg catgctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     2401 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     2461 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     2521 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     2581 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     2641 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     2701 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     2761 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     2821 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     2881 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     2941 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     3001 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     3061 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     3121 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     3181 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     3241 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     3301 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     3361 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     3421 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     3481 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
     3541 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
     3601 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
     3661 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     3721 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     3781 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     3841 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     3901 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
     3961 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     4021 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4081 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     4141 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4201 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat
     4261 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg
     4321 ggcgtgaatt ttcgctgctt gtccttttcc tgcatgctgg ttgctcccat tcttaggtga
     4381 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     4441 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     4501 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     4561 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     4621 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     4681 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     4741 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     4801 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     4861 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     4921 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     4981 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     5041 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     5101 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     5161 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     5221 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     5281 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     5341 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     5401 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     5461 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     5521 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     5581 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc
     5641 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     5701 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     5761 cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     5821 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
     5881 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     5941 ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
     6001 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     6061 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     6121 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
     6181 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
     6241 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg
     6301 ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt
     6361 tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc
     6421 ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc
     6481 ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc
     6541 ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag
     6601 atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc
     6661 cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct
     6721 gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa
     6781 aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc
     6841 tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct
     6901 tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt
     6961 gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
     7021 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7081 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7141 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7201 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7261 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7321 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7381 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7441 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7501 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7561 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7621 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7681 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7741 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7801 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7861 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7921 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7981 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8041 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tgcgccgcgt
     8881 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     8941 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     9001 gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg
     9061 gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc
     9121 gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta
     9181 agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
     9241 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
     9301 gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg
     9361 ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
     9421 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
     9481 aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag
     9541 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac
     9601 agcgtgccgc agatcccggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     9661 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     9721 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     9781 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     9841 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     9901 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     9961 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
    10021 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
    10081 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
    10141 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
    10201 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
    10261 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
    10321 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
    10381 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
    10441 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
    10501 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
    10561 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
    10621 gacgccccag cactcgtccg agggcaaagg aataggggag atgggggagg ctaactgaaa
    10681 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
    10741 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
    10801 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
    10861 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
    10921 ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt
    10981 tatggttcgt gggggttatt attttgggcg ttgcgtgggg tctggtccac gactggactg
    11041 agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca
    11101 cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat
    11161 ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
    11221 gggcaacgtt gttgcattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
    11281 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
    11341 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
    11401 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
    11461 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    11521 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    11581 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    11641 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
    11701 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
    11761 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    11821 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    11881 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
    11941 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    12001 agctcgttta gtgaaccgtc agatctctag aagctgggta ccagct
//
