LOCUS       sequence-26157-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-HA2-DDB1
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atgtacccat acgatgttcc tgactatgcg ggctatccct
      121 atgacgtccc ggactatgca agcttggtac cgttaacatg gatccgcatg tcgtacaact
      181 acgtggtaac ggcccagaag cccaccgccg tgaacggctg cgtgaccgga cactttactt
      241 cggccgaaga cttaaacctg ttgattgcca aaaacacgag attagagatc tatgtggtca
      301 ccgccgaggg gcttcggcct gtcaaagagg tgggcatgta tgggaagatt gcggtcatgg
      361 agcttttcag gcccaagggg gagagcaagg acctgctgtt tatcttgaca gcgaagtaca
      421 atgcctgcat cctggagtat aaacagagtg gcgagagcat tgacatcatt acgcgagccc
      481 atggcaatgt ccaggaccgc attggccgcc cctcagagac cggcattatt ggcatcattg
      541 accctgagtg ccggatgatt ggcctgcgtc tctatgatgg ccttttcaag gttattccac
      601 tagatcgcga taataaagaa ctcaaggcct tcaacatccg cctggaggag ctgcatgtca
      661 ttgatgtcaa gttcctatat ggttgccaag cacctactat ttgctttgtc taccaggacc
      721 ctcaggggcg gcacgtaaaa acctatgagg tgtctctccg agaaaaggaa ttcaataagg
      781 gcccttggaa acaggaaaat gtc
//
