LOCUS       sequence-261574-       11833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             70..93
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1466..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1490..1509)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1544..1563
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1742..1764)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2350..4139
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4445..6370)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6805..6823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6891..6995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6996..7064,7065..7856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7214..7233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8027..8615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8516..8535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9232..9377
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9417..10442
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9417..10442
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10463..10482)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10507..10555
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11208..11587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11588..11791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11742..11762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11788..11812
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgggcacac aggagggctg gtgcctgctg ctctgcctgg ctctatctgg agcagcagaa
       61 accggctccg actacaagga cgatgatgac aagggaggat ccaagcccca cccagcagag
      121 gggcagtggc gggcagtgga cgtggtccta gactgtttcc tggtgaagga cggtgcgcac
      181 cgtggagctc tcgccagcag tgaggacagg gcaagggcct cccttgtgct gaagcaggtg
      241 ccagtgctgg acgatggctc cctggaggac ttcaccgatt tccaaggggg cacactggcc
      301 caagatgacc cacctattat ctttgaggcc tcagtggacc tggtccagat tccccaggcc
      361 gaggccttgc tccatgctga ctgcagtggg aaggaggtga cctgtgagat ctcccgctac
      421 tttctccaga tgacagagac cactgttaag acagcagctt ggttcatggc caacgtgcag
      481 gtctctggag ggggacctag catctccttg gtgatgaaga ctcccagggt cgccaagaat
      541 gaggtgctct ggcacccaac gctgaacttg ccactgagcc cccaggggac tgtgcgaact
      601 gcagtggagt tccaggtgat gacacagacc caatccctga gcttcctgct ggggtcctca
      661 gcctccttgg actgtggctt ctccatggca ccgggcttgg acctcatcag tgtggagtgg
      721 cgactgcagc acaagggcag gggtcagttg gtgtacagct ggaccgcagg gcaggggcag
      781 gctgtgcgga agggcgctac cctggagcct gcacaactgg gcatggccag ggatgcctcc
      841 ctcaccctgc ccggcctcac tatacaggac gaggggacct acatttgcca gatcaccacc
      901 tctctgtacc gagctcagca gatcatccag ctcaacatcc aagcttcccc taaagtacga
      961 ctgagcttgg caaacgaagc tctgctgccc accctcatct gcgacattgc tggctattac
     1021 cctctggatg tggtggtgac gtggacccga gaggagctgg gtggatcccc agcccaagtc
     1081 tctggtgcct ccttctccag cctcaggcaa agcgtggcag gcacctacag catctcctcc
     1141 tctctcaccg cagaacctgg ctctgcaggt gccacttaca cctgccaggt cacacacatc
     1201 tctctggagg agccccttgg ggccagcacc caggttgtcc caccagagcg gagaacagcc
     1261 ttgggagtca tctttgccag cagtctcttc cttcttgcac tgatgttcct ggggcttcag
     1321 agacggcaag cacctacagg acttgggctg cttcaggctg aacgctggga gaccacttcc
     1381 tgtgctgaca cacagagctc ccatctccat gaagaccgca cagcgcgtgt aagccagccc
     1441 agctaactcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg
     1501 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1561 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1621 ttttatgttt caggttcagg gggaggtggg gaggtttttt aaagcaagta aaacctctac
     1681 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     1741 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     1801 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     1861 ccggtcgact ctagagcccc gccgccggac gaactaaacc tgactacggc atctctgccc
     1921 cttcttcgct ggtacgagga gcgcttttgt tttgtattcg gggcagtgca tgtaatccct
     1981 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     2041 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     2101 ccaacactga gtggctttca tcctggagca gactttgcat gctgtggact gcaacacaac
     2161 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     2221 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     2281 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     2341 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     2401 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     2461 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     2521 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     2581 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     2641 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     2701 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     2761 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     2821 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     2881 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     2941 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     3001 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     3061 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     3121 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     3181 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     3241 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     3301 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     3361 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     3421 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     3481 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3541 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     3601 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     3661 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     3721 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     3781 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     3841 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     3901 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     3961 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4021 ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc
     4081 tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc
     4141 atgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
     4201 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
     4261 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
     4321 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
     4381 attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt
     4441 tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc
     4501 cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta
     4561 ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca
     4621 taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga
     4681 ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg
     4741 ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag
     4801 ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga
     4861 cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac
     4921 cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc
     4981 accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt
     5041 ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat
     5101 ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg
     5161 gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg
     5221 gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc
     5281 cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc
     5341 ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg
     5401 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc
     5461 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     5521 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5581 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     5641 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     5701 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
     5761 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     5821 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5881 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     5941 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     6001 cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     6061 ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc
     6121 ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg
     6181 gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc
     6241 cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt
     6301 ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct
     6361 cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt
     6421 gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca
     6481 tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc
     6541 tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc
     6601 tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg
     6661 gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca
     6721 acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac
     6781 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     7261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7501 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     7561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8161 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8641 ggttcctggc cttttgctgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     8701 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     8761 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     8821 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     8881 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     8941 ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     9001 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     9061 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     9121 acgtagccca gcgcgtcggc cgccatgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     9181 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     9241 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     9301 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     9361 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggcaa tgagatatga
     9421 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     9481 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     9541 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     9601 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     9661 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     9721 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     9781 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     9841 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     9901 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     9961 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
    10021 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
    10081 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
    10141 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
    10201 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
    10261 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    10321 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    10381 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    10441 aggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    10501 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    10561 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    10621 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    10681 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
    10741 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
    10801 cgtggggtct ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
    10861 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
    10921 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
    10981 tgtttgacag cttatcatcg cagatccggg caacgttgtt gcattgctgc aggcgcagaa
    11041 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
    11101 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
    11161 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
    11221 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    11281 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11341 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    11401 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    11461 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    11521 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    11581 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
    11641 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    11701 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    11761 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag
    11821 ctgggtacca gct
//
