LOCUS       sequence-26158-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-HA3-TSC1
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc tatggcccaa caagcaaatg tcggggagct
      181 tcttgccatg ctggactccc ccatgctggg tgtgcgggac gacgtgacag ctgtctttaa
      241 agagaacctc aattctgacc gtggccctat gcttgtaaac accttggtgg attattacct
      301 ggaaaccagc tctcagccgg cattgcacat cctgaccacc ttgcaagagc cacatgacaa
      361 gcacctcttg gacaggatta acgaatatgt gggcaaagcc gccactcgtt tatccatcct
      421 ctcgttactg ggtcatgtca taagactgca gccatcttgg aagcataagc tctctcaagc
      481 acctcttttg ccttctttac taaaatgtct caagatggac actgacgtcg ttgtcctcac
      541 aacaggcgtc ttggtgttga taaccatgct accaatgatt ccacagtctg ggaaacagca
      601 tcttcttgat ttctttgaca tttttggccg tctgtcatca tggtgcctga agaaaccagg
      661 ccacgtggcg gaagtctatc tcgtccatct ccatgccagt gtgtacgcac tctttcatcg
      721 cctttatgga atgtaccctt gcaacttcgt ctcctttttg cgttctcatt acagtatgaa
      781 agaaaacctg gagacttttg aa
//
