LOCUS       sequence-261588-        9861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Reinhard NR, Van Der Niet S, Chertkova A, Postma M, Hordijk PL, 
            Gadella TWJ Jr, Goedhart J
  TITLE     Identification of guanine nucleotide exchange factors that increase 
            Cdc42 activity in primary human endothelial cells.
  JOURNAL   Small GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509.
  PUBMED    31469028
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small 
            GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509."
COMMENT     Sequence Label: mCherry-ITSN1
FEATURES             Location/Qualifiers
     source          1..9861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6649..6770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6777..7232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6914..6935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7126..7145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7259..7363
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7365..7722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7365..7556
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7388..7408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7757..8551
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7757..8551
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7811..7830)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8421..8440
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8739..8758)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8783..8830
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9159..9747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9648..9667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc cgctcagttt ccaacacctt ttggtggcag cctggatatc
     1381 tgggccataa ctgtagagga aagagcgaag catgatcagc agttccatag tttaaagcca
     1441 atatctggat tcattactgg tgatcaagct agaaactttt tttttcaatc tgggttacct
     1501 caacctgttt tagcacagat atgggcacta gctgacatga ataatgatgg aagaatggat
     1561 caagtggagt tttccatagc tatgaaactt atcaaactga agctacaagg atatcagcta
     1621 ccctctgcac ttccccctgt catgaaacag caaccagttg ctatttctag cgcaccagca
     1681 tttggtatgg gaggtatcgc cagcatgcca ccgcttacag ctgttgctcc agtgccaatg
     1741 ggatccattc cagttgttgg aatgtctcca accctagtat cttctgttcc cacagcagct
     1801 gtgccccccc tggctaacgg ggctccccct gttatacaac ctctgcctgc atttgctcat
     1861 cctgcagcca cattgccaaa gagttcttcc tttagtagat ctggtccagg gtcacaacta
     1921 aacactaaat tacaaaaggc acagtcattt gatgtggcca gtgtcccacc agtggcagag
     1981 tgggctgttc ctcagtcatc aagactgaaa tacaggcaat tattcaatag tcatgacaaa
     2041 actatgagtg gacacttaac aggtccccaa gcaagaacta ttcttatgca gtcaagttta
     2101 ccacaggctc agctggcttc aatatggaat ctttctgaca ttgatcaaga tggaaaactt
     2161 acagcagagg aatttatcct ggcaatgcac ctcattgatg tagctatgtc tggccaacca
     2221 ctgccacctg tcctgcctcc agaatacatt ccaccttctt ttagaagagt tcgatctggc
     2281 agtggtatat ctgtcataag ctcaacatct gtagatcaga ggctaccaga ggaaccagtt
     2341 ttagaagatg agcaacaaca attagaaaag aaattacctg taacgtttga agataagaag
     2401 cgggagaact ttgaacgtgg caacctggaa ctggagaaac gaaggcaagc tctcctggaa
     2461 cagcagcgca aggagcagga gcgcctggcc cagctggagc gggcggagca ggagaggaag
     2521 gagcgtgagc gccaggagca agagcgcaaa agacaactgg aactggagaa gcaactggaa
     2581 aagcagcggg agctagaacg gcagagagag gaggagagga ggaaagaaat tgagaggcga
     2641 gaggctgcaa aacgggaact tgaaaggcaa cgacaacttg agtgggaacg gaatcgaagg
     2701 caagaactac taaatcaaag aaacaaagaa caagaggaca tagttgtact gaaagcaaag
     2761 aaaaagactt tggaatttga attagaagct ctaaatgata aaaagcatca actagaaggg
     2821 aaacttcaag atatcagatg tcgattgacc acccaaaggc aagaaattga gagcacaaac
     2881 aaatctagag agttgagaat tgccgaaatc acccatctac agcaacaatt acaggaatct
     2941 cagcaaatgc ttggaagact tattccagaa aaacagatac tcaatgacca attaaaacaa
     3001 gttcagcaga acagtttgca cagagattca cttgttacac ttaaaagagc cttagaagca
     3061 aaagaactag ctcggcagca cctacgagac caactggatg aagtggagaa agaaactaga
     3121 tcaaaactac aggagattga tattttcaat aatcagctga aggaactaag agaaatacac
     3181 aataagcaac aactccagaa gcaaaagtcc atggaggctg aacgactgaa acagaaagaa
     3241 caagaacgaa agatcataga attagaaaaa caaaaagaag aagcccaaag acgagctcag
     3301 gaaagggaca agcagtggct ggagcatgtg cagcaggagg acgagcatca gagaccaaga
     3361 aaactccacg aagaggaaaa actgaaaagg gaggagagtg tcaaaaagaa ggatggcgag
     3421 gaaaaaggca aacaggaagc acaagacaag ctgggtcggc ttttccatca acaccaagaa
     3481 ccagctaagc cagctgtcca ggcaccctgg tccactgcag aaaaaggtcc acttaccatt
     3541 tctgcacagg aaaatgtaaa agtggtgtat taccgggcac tgtacccctt tgaatccaga
     3601 agccatgatg aaatcactat ccagccagga gacatagtca tggttaaagg ggaatgggtg
     3661 gatgaaagcc aaactggaga acccggctgg cttggaggag aattaaaagg aaagacaggg
     3721 tggttccctg caaactatgc agagaaaatc ccagaaaatg aggttcccgc tccagtgaaa
     3781 ccagtgactg attcaacatc tgcccctgcc cccaaactgg ccttgcgtga gacccccgcc
     3841 cctttggcag taacctcttc agagccctcc acgaccccta ataactgggc cgacttcagc
     3901 tccacgtggc ccaccagcac gaatgagaaa ccagaaacgg ataactggga tgcatgggca
     3961 gcccagccct ctctcaccgt tccaagtgcc ggccagttaa ggcagaggtc cgcctttact
     4021 ccagccacgg ccactggctc ctccccgtct cctgtgctag gccagggtga aaaggtggag
     4081 gggctacaag ctcaagccct atatccttgg agagccaaaa aagacaacca cttaaatttt
     4141 aacaaaaatg atgtcatcac cgtcctggaa cagcaagaca tgtggtggtt tggagaagtt
     4201 caaggtcaga agggttggtt ccccaagtct tacgtgaaac tcatttcagg gcccataagg
     4261 aagtctacaa gcatggattc tggttcttca gagagtcctg ctagtctaaa gcgagtagcc
     4321 tctccagcag ccaagccggt cgtttcggga gaagaattta ttgccatgta cacttacgag
     4381 agttctgagc aaggagattt aacctttcag caaggggatg tgattttggt taccaagaaa
     4441 gatggtgact ggtggacagg aacagtgggc gacaaggccg gagtcttccc ttctaactat
     4501 gtgaggctta aagattcaga gggctctgga actgctggga aaacagggag tttaggaaaa
     4561 aaacctgaaa ttgcccaggt tattgcctca tacaccgcca ccggccccga gcagctcact
     4621 ctcgcccctg gtcagctgat tttgatccga aaaaagaacc caggtggatg gtgggaagga
     4681 gagctgcaag cacgtgggaa aaagcgccag ataggctggt tcccagctaa ttatgtaaag
     4741 cttctaagcc ctgggacgag caaaatcact ccaacagagc cacctaagtc aacagcatta
     4801 gcggcagtgt gccaggtgat tgggatgtac gactacaccg cgcagaatga cgatgagctg
     4861 gccttcaaca agggccagat catcaacgtc ctcaacaagg aggaccctga ctggtggaaa
     4921 ggagaagtca atggacaagt ggggctcttc ccatccaatt atgtgaagct gaccacagac
     4981 atggacccaa gccagcaatg gtgttcagac ttacatctct tggatatgtt gaccccaact
     5041 gaaagaaagc gacaaggata catccacgag ctcattgtca ccgaggagaa ctatgtgaat
     5101 gacctgcagc tggtcacaga gatttttcaa aaacccctga tggagtctga gctgctgaca
     5161 gaaaaagagg ttgctatgat ttttgtgaac tggaaggagc tgattatgtg taatatcaaa
     5221 ctactaaaag cgctgagagt ccgcaagaag atgtccgggg agaagatgcc tgtgaagatg
     5281 attggagaca tcctgagcgc acagctgccg cacatgcagc cctacatccg cttctgcagc
     5341 cgccagctca acggggctgc cctgatccag cagaagacgg acgaggcccc agacttcaag
     5401 gagttcgtca aaagattggc aatggatcct cggtgtaaag ggatgccact ctctagtttt
     5461 atactgaagc ctatgcaacg ggtaacaaga tacccactga tcattaaaaa tatcctggaa
     5521 aacacccctg aaaaccaccc ggaccacagc cacttgaagc acgccctgga gaaggcggaa
     5581 gagctctgtt cccaggtgaa cgaaggggtg cgggagaagg agaactctga ccggctggag
     5641 tggatccagg cccacgtgca gtgtgaaggc ctgtctgagc aacttgtgtt caattcagtg
     5701 accaattgct tggggccgcg caaatttctg cacagtggga agctctacaa ggccaagagc
     5761 aacaaggagc tgtatggctt ccttttcaac gacttcctcc tgctgactca gatcacgaag
     5821 cctttggggt cttctggcac cgacaaagtc ttcagcccca aatcaaacct gcagtataaa
     5881 atgtataaaa cacctatttt cctaaatgag gttctagtaa aattacccac cgacccttct
     5941 ggagacgagc ccatcttcca catctcccac attgaccgcg tctatactct ccgagcagaa
     6001 agcataaatg aaaggactgc ctgggtgcag aaaatcaaag ctgcttctga actctacata
     6061 gagactgaga aaaagaagcg cgagaaagcg tacctggtcc gttcccaaag ggcaacaggc
     6121 attggaaggt tgatggtgaa cgtggttgaa ggcatcgagt tgaaaccctg tcggtcacat
     6181 ggaaagagca acccgtactg tgaggtgacc atgggttccc agtgccacat caccaagacg
     6241 atccaggaca ctctgaaccc caagtggaat tccaactgcc agttcttcat ccgagacctg
     6301 gagcaggaag tcctctgcat cactgtgttc gagagggacc agttctcacc agatgatttt
     6361 ttgggtcgga cggagatccg tgtggcggac atcaagaaag accagggctc caaaggtcca
     6421 gttacgaagt gtcttctgct gcacgaagtc cccacgggag agattgtggt ccgcttggac
     6481 ctgcagttgt ttgatgagcc gtaaggtacc gcgggcccgg gatccaccgg atctagataa
     6541 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     6601 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     6781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7201 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     7261 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7321 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     7381 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7441 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     7741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7921 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     8161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     8221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     8281 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     8341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8521 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8581 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8701 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     8761 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     8821 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     8881 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     8941 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     9001 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9841 aaccgtatta ccgccatgca t
//
