LOCUS       sequence-26159-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pCDNA3-myc3-FBW5 delta F
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atggagcaaa agctcatctc agaggaagat ctggaattag
      121 agcaaaagct catctcagag gaagatctgg aattagagca aaagctcatc tcagaggaag
      181 atctggaatt catgtggagg gagcagttct accgctacta ccaggtggcc cgcgacgtgc
      241 cccgacaccc agcggccatg tcctggtacg aggagttcca gcggctgtat gacacggtgc
      301 cctgcgtgga ggtgcagacg ctgcgggaac acacagacca ggtcctgcac ctcagcttct
      361 cccattccgg gtaccagttc gcgtcctgct ccaaggactg cactgtgaag atctggagca
      421 acgacctgac catctcgctg ctgcacagcg cggacatgcg gccctacaac tggagctaca
      481 cccagttctc ccagttcaac aaggacgact cgctactgct ggcctcgggg gtgttcctgg
      541 ggccgcacaa ctcctcatcc ggcgagattg ctgtcatcag cctagactcc ttcgcgctgc
      601 tgtcccgcgt gcggaacaag ccctatgacg tgtttggctg ttggctcacc gagaccagcc
      661 tcatctcggg gaacctgcac cgcatcggag atatcacctc ctgctcggtg ctgtggctca
      721 acaatgcctt ccaggatgtg gagtcagaga acgtcaacgt ggtgaagcgg ctgttcaaga
      781 tccagaacct caatgccagc accgtccgca cggtgatggt ggccgactgc agccgcttcg
      841 acagccctga cctgctgctg gaagccgg
//
