LOCUS       sequence-261594-        9728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Reinhard NR, Van Der Niet S, Chertkova A, Postma M, Hordijk PL, 
            Gadella TWJ Jr, Goedhart J
  TITLE     Identification of guanine nucleotide exchange factors that increase 
            Cdc42 activity in primary human endothelial cells.
  JOURNAL   Small GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509.
  PUBMED    31469028
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small 
            GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509."
COMMENT     Sequence Label: mCherry-PREX1
FEATURES             Location/Qualifiers
     source          1..9728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6516..6637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6553..6572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6607..6626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6644..7099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6781..6802)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6993..7012
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7126..7230
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7232..7589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7232..7423
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7255..7275)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7440..7575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7624..8418
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7624..8418
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7678..7697)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8288..8307
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8606..8625)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8650..8697
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9026..9614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9515..9534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcc gaattcatgg aggcgcccag cggcagcgag
     1381 cccggcggcg acggggccgg ggactgcgcc cacccggacc cccgggcccc tggcgccgcg
     1441 gcgcccagct ccggccccgg cccgtgcgcg gccgcccggg agtccgagcg ccagctgcgc
     1501 ctccgcctct gcgtcctcaa cgagatcttg ggcaccgaga gggactacgt gggcaccttg
     1561 cgcttcttgc agtcggcatt cctgcatcgc atccggcaga acgtggccga ctcagtggag
     1621 aagggcctca cggaggagaa tgtcaaggtc ctgttctcga acatcgaaga catcctggaa
     1681 gttcataagg atttcttggc cgccttggag tattgtttac acccggagcc gcagtctcag
     1741 catgaacttg ggaatgtttt cttaaaattc aaggacaagt tctgcgtgta cgaggagtat
     1801 tgcagcaacc atgagaaagc cctgaggctg ctggtggagc tgaacaagat ccctaccgtg
     1861 cgcgccttcc ttttgagctg catgcttctg ggaggccgga agaccacgga catccctttg
     1921 gaaggctacc tgttgtctcc gatccagagg atctgcaagt acccgctcct ccttaaggag
     1981 ctggccaaga ggactcccgg caagcaccca gaccaccccg cggtccagag tgccctgcag
     2041 gccatgaaga ccgtttgctc caacatcaat gagaccaagc ggcagatgga gaagctggaa
     2101 gccctggagc agctgcagtc ccacatcgaa ggctgggagg gttccaacct cacagacatc
     2161 tgcactcagc tcctcctgca agggactttg ttaaagatct ctgcgggcaa catccaggaa
     2221 agggccttct tcctcttcga caaccttctc gtctactgca agcggaaatc cagggtcacc
     2281 gggagcaaga agtccaccaa gaggaccaaa tccatcaacg gctccctcta catcttcagg
     2341 ggtcgaatca acactgaagt catggaggtg gagaatgtgg aagatgggac agcggattac
     2401 catagcaacg gctataccgt caccaacggc tggaagatcc acaacacggc caagaataag
     2461 tggtttgtct gcatggccaa gacggcagag gagaagcaga agtggctgga tgccatcatc
     2521 cgcgagcggg agcagcgcga gagcctgaag ctgggcatgg agcgtgatgc ctacgtcatg
     2581 attgcggaga agggggagaa gctgtaccac atgatgatga acaagaaggt gaacctcatc
     2641 aaggaccgcc ggagaaagct gagcactgtc cccaagtgct ttcttggcaa tgagttcgtt
     2701 gcctggctcc tagaaattgg tgaaatcagc aagacggaag aaggagtcaa cttgggccaa
     2761 gccctgttgg agaatggcat catccaccat gtttccgaca agcaccagtt caagaatgag
     2821 caggtgatgt atcgcttccg ctacgacgat ggcacctaca aggcccgaag tgagctggag
     2881 gacatcatgt ccaagggtgt gaggctttac tgccgtcttc acagcctcta caccccggtg
     2941 atcaaagacc gtgattacca cctgaagacc tacaagtcag tgcttcccgg gagcaagctg
     3001 gtggactggc tgctggctca gggagactgc cagactcggg aggaggcagt ggcgctcggc
     3061 gtgggtctgt gcaacaatgg cttcatgcac cacgtgctgg agaagagcga gttcagggat
     3121 gagtcccagt acttccgctt tcatgctgac gaggagatgg aggggaccag cagcaagaac
     3181 aaacagcttc gcaacgactt caagctggtg gagaacattc tggccaagcg cctgctgatc
     3241 ctgccccagg aggaggacta tggctttgac atcgaggaga agaacaaggc tgtggtggtg
     3301 aagtccgtcc agaggggctc gctggctgag gtggctggcc tgcaggtggg gaggaagatc
     3361 tactccatca atgaggacct ggtgttcctg cggccgtttt cagaggtgga gtccatcctc
     3421 aaccagtcct tctgctcccg ccgccctctg cgcctcctgg tggccacgaa ggccaaagag
     3481 atcatcaaaa tccccgacca gccggacaca ctgtgcttcc agattcgtgg agctgcccca
     3541 ccgtacgtct atgctgtggg gagaggctct gaggccatgg ctgcagggct ctgtgctggt
     3601 cagtgcattc tgaaggtcaa tggcagcaac gtgatgaacg atggtgcccc tgaggtcctg
     3661 gagcacttcc aggcattccg gagtcggcgc gaagaggccc tgggcctgta ccagtggatc
     3721 taccacaccc atgaggatgc ccaggaagca cgagccagtc aggaggcctc cactgaggac
     3781 cccagtggcg agcaggccca ggaggaagac caggctgatt cagccttccc actgctgtcc
     3841 ctgggtcccc ggctgagcct gtgtgaggac agccccatgg tcaccctgac tgtggacaac
     3901 gtgcacctgg aacacggcgt ggtgtatgag tatgtgagca cggcaggcgt caggtgccat
     3961 gtgctggaga agatcgtgga gccccgcggc tgcttcggcc tcaccgccaa gatcctcgag
     4021 gcctttgctg ccaatgacag cgtcttcgtg gagaactgca ggcggctcat ggccctgagc
     4081 agcgccatcg tgaccatgcc ccactttgag ttccgcaaca tctgtgacac caagctggag
     4141 agcattggcc agaggattgc ctgctaccag gagtttgcag cccaactgaa gagcagggtc
     4201 agcccaccct tcaaacaagc ccccctggag ccccacccgc tgtgtggcct ggacttctgc
     4261 cccaccaatt gccacatcaa cctcatggaa gtgtcctacc ccaagaccac cccctcagtg
     4321 ggcaggtcct tcagcatccg ctttggacgc aaaccctccc tcatcggcct tgacccggag
     4381 caaggccacc tgaaccccat gtcgtacacc cagcactgca tcaccaccat ggctgctccc
     4441 tcctggaagt gcttgcctgc tgcagagggt gatccccaag gccagggtct ccatgatggc
     4501 agcttcgggc cagccagtgg gacccttggt caggaagacc ggggcctcag cttcctactc
     4561 aagcaggagg accgtgagat ccaggatgcc tacctgcagc tcttcaccaa gctggatgtg
     4621 gccctgaagg agatgaagca atatgtcacc cagatcaaca ggctgctgtc caccatcaca
     4681 gagcccacct cgggtgggtc ctgcgacaca tccttggctg aggaggcctc ctccctgccc
     4741 ctggtcagtg aagagagcga gatggacagg agtgaccatg ggggcatcaa gaaggtgtgc
     4801 ttcaaggtgg ccgaggagga ccaggaggac tcaggccacg acaccatgag ttatcgcgac
     4861 tcctacagcg agtgtaacag caatcgagac tcggtcctgt cctacaccag cgtgagaagt
     4921 aacagctcct acttgggcag cgacgagatg gggtctggag atgagctgcc ctgtgacatg
     4981 cggatcccat ctgacaagca ggacaagctt catggctgcc tggagcacct ctttaaccag
     5041 gtggactcca tcaatgctct cctcaagggg ccagtcatga gccgggcttt cgaagagacc
     5101 aagcatttcc ctatgaacca cagcttacaa gagtttaaac agaaagaaga gtgtacaatc
     5161 cgtggccgga gcctgatcca gattagcatc caggaggacc cctggaacct ccccaactcc
     5221 atcaagaccc tggtggacaa cattcagaga tatgtggaag atgggaagaa ccagctgctc
     5281 ctggccttgc tgaagtgcac agacacggag ctgcagctgc gcagagacgc gatcttctgc
     5341 caggccctgg tggccgccgt gtgcaccttc tccgagcagc tgctggcggc cctgggctac
     5401 cgctacaaca acaatggcga gtacgaggag agcagccgcg acgccagccg caagtggctg
     5461 gagcaggtgg cggccacggg tgtcctgctg cactgccagt ccctgctctc gccagccaca
     5521 gtgaaggagg aacggaccat gctggaggac atctgggtga cgctgtcaga gctggacaat
     5581 gtcaccttct cctttaagca gctggacgag aactatgtgg ccaacaccaa cgtcttctac
     5641 cacattgagg gcagccggca ggcgctgaag gtcatcttct acctcgacag ctaccacttc
     5701 tccaagctgc cctcccgcct ggagggtggg gccagcctga ggctgcacac agcgctgttc
     5761 acgaaagtgc tggagaacgt ggaggggctg ccttctccag gcagccaggc cgcggaggat
     5821 ttgcagcagg acatcaacgc gcagtccctg gagaaagttc agcagtatta ccgcaaactc
     5881 agggcatttt acctggagcg gtctaacctg cccacggatg ccagcaccac ggcggtaaag
     5941 atagaccagc tgatccgccc catcaatgcc ctggatgagc tctgccgcct catgaagtcc
     6001 tttgtccacc caaagcctgg tgctgctggg agtgtgggcg ccggcctcat ccccatctcc
     6061 tcggagctct gctaccgcct gggggcctgc cagatggtca tgtgtggcac aggcatgcag
     6121 aggagcaccc tgagcgtgtc cctggagcag gcggccatct tggcacggag ccacgggttg
     6181 ctgcccaagt gcatcatgca ggccacggac atcatgcgga agcagggccc aagggtggag
     6241 attctggcca aaaacctgcg agtcaaggac cagatgcccc agggtgctcc gcgcctctac
     6301 cgcctctgcc agccgccggt ggatggggac ctctgaacac ccaaatgccc cacgctgggc
     6361 cgcggctctg gagctgggat ttgggaggac acagaatcac tagataactg atcataatca
     6421 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6481 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     6661 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6721 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6781 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6841 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     6901 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     6961 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7021 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7081 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7201 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     7261 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7321 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7381 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7441 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7501 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7561 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     7621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7801 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     8161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8581 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     8641 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     8701 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     8761 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     8821 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9721 ccatgcat
//
