LOCUS       sequence-261598-       11524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-B*07:02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B7
FEATURES             Location/Qualifiers
     source          1..11524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1157..1291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1181..1200)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1235..1254
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1433..1455)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2041..3830
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4136..6061)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6496..6514)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6582..6686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6687..6755,6756..7547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6905..6924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7718..8306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8207..8226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8923..9068
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9108..10133
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9108..10133
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10154..10173)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10198..10246
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        10899..11278
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11279..11482
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11433..11453
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11479..11503
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcagttc aggaggcgga
      121 tctcattcta tgagatattt ctacacctcc gtgtcccggc ccggccgcgg ggagccccgc
      181 ttcatctcag tgggctacgt ggacgacacc cagttcgtga ggttcgacag cgacgccgcg
      241 agtccgagag aggagccgcg ggcgccgtgg atagagcagg aggggccgga gtattgggac
      301 cggaacacac agatctacaa ggcccaggca cagactgacc gagagagcct gcggaacctg
      361 cgcggctact acaaccagag cgaggccggg tctcacaccc tccagagcat gtacggctgc
      421 gacgtggggc cggacgggcg cctcctccgc gggcatgacc agtacgccta cgacggcaag
      481 gattacatcg ccctgaacga ggacctgcgc tcctggaccg ccgcggacac ggcggctcag
      541 atcacccagc gcaagtggga ggcggcccgt gaggcggagc agcggagagc ctacctggag
      601 ggcgagtgcg tggagtggct ccgcagatac ctggagaacg ggaaggacaa gctggagcgc
      661 gctgaccccc caaagacaca cgtgacccac caccccatct ctgaccatga ggccaccctg
      721 aggtgctggg ccctgggttt ctaccctgcg gagatcacac tgacctggca gcgggatggc
      781 gaggaccaaa ctcaggacac tgagcttgtg gagaccagac cagcaggaga tagaaccttc
      841 cagaagtggg cagctgtggt ggtgccttct ggagaagagc agagatacac atgccatgta
      901 cagcatgagg ggctgccgaa gcccctcacc ctgagatggg agccgtcttc ccagtccaca
      961 gtgcccatcg ttggaatcgt tgcaggactg gcagttctcg ccgtagtggt tataggagca
     1021 gtggtcgctg ctgttatgtg tcgcaggaaa agttcaggcg gcaagggagg atcttactca
     1081 caagcggcgt gctccgatag cgcacaaggc tcagatgttt ctctgacggc atgataactc
     1141 gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     1201 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1261 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     1321 tcaggttcag ggggaggtgg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1381 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1441 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1501 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg accggtcgac
     1561 tctagagccc cgccgccgga cgaactaaac ctgactacgg catctctgcc ccttcttcgc
     1621 tggtacgagg agcgcttttg ttttgtattc ggggcagtgc atgtaatccc ttcagttggt
     1681 tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa
     1741 aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg
     1801 agtggctttc atcctggagc agactttgca tgctgtggac tgcaacacaa cattgccttt
     1861 atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg
     1921 cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga
     1981 taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta
     2041 aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa
     2101 tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct
     2161 aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg
     2221 attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg
     2281 ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga
     2341 ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg
     2401 tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc
     2461 aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga
     2521 atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc
     2581 tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta
     2641 ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt
     2701 aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc
     2761 cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact
     2821 cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt
     2881 ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg
     2941 tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt
     3001 gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc
     3061 gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg
     3121 tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc
     3181 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     3241 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     3301 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     3361 ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc
     3421 ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     3481 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     3541 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     3601 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     3661 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca
     3721 tgcatataca gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat
     3781 gccgccacct cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg catgctggtt
     3841 gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct
     3901 caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt
     3961 gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag
     4021 acagatggcc agaaatacac caacagcacg catgatgtct actggggatt tattctttag
     4081 tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact
     4141 cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac
     4201 cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt
     4261 caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt
     4321 ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat
     4381 aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa
     4441 aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc
     4501 ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc
     4561 cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa
     4621 tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt
     4681 ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt
     4741 catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac
     4801 cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac
     4861 ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg
     4921 gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg
     4981 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
     5041 ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc
     5101 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     5161 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctc
     5221 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgctcctg
     5281 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgctc
     5341 ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     5401 ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct gctcctgccc
     5461 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     5521 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     5581 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc
     5641 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     5701 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc
     5761 ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc
     5821 caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc
     5881 ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc
     5941 gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag
     6001 atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca
     6061 tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg
     6121 gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg
     6181 tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca
     6241 attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca
     6301 agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg
     6361 gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa
     6421 aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt
     6481 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6541 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6901 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     6961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7201 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     7261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7861 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8341 ccttttgctg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
     8401 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
     8461 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
     8521 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc
     8581 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg
     8641 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca
     8701 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     8761 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
     8821 agcgcgtcgg ccgccatgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg
     8881 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc
     8941 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg
     9001 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac
     9061 ccgcttaaca gcgtcaacag cgtgccgcag atcccgggca atgagatatg aaaaagcctg
     9121 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     9181 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     9241 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     9301 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     9361 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     9421 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     9481 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     9541 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     9601 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     9661 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     9721 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     9781 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     9841 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     9901 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     9961 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    10021 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    10081 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taggggagat
    10141 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
    10201 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
    10261 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
    10321 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
    10381 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
    10441 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
    10501 tggtccacga ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc
    10561 gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc
    10621 aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc atgtttgaca
    10681 gcttatcatc gcagatccgg gcaacgttgt tgcattgctg caggcgcaga actggtaggt
    10741 atggaagatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat
    10801 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta
    10861 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta
    10921 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
    10981 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    11041 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    11101 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
    11161 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    11221 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    11281 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
    11341 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    11401 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
    11461 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atctctagaa gctgggtacc
    11521 agct
//
