LOCUS       sequence-261617-        7238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein mKate(KPS12) variant for optimized expression in
            Bacillus mycoides..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Yi Y, Frenzel E, Spoelder J, Elzenga JTM, van Elsas JD, Kuipers OP
  TITLE     Optimized fluorescent proteins for the rhizosphere-associated 
            bacterium Bacillus mycoides with endophytic and biocontrol agent 
            potential.
  JOURNAL   Environ Microbiol Rep. 2018 Feb;10(1):57-74. doi: 
            10.1111/1758-2229.12607. Epub 2018 Jan 5.
  PUBMED    29195004
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1758-2229.12607"; journalName: "Environ Microbiol Rep"; 
            date: "2018-02"; volume: "10"; issue: "1"; pages: "57-74"
COMMENT     Sequence Label: pAD-mKate(KPS12)
FEATURES             Location/Qualifiers
     source          1..7238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2253..2272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    5139..5279
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5294..5311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5465..6053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5545..5564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6224..7015,7016..7084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6847..6866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7085..7189)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcgag
       61 ctcggtaccc ggggatcacs tgaargaaat tttcggcgca gagcacgtaa atgttcaacc
      121 acactctggt gcacaagcga acatggcagt atacttcacg attttagagc aaggcgatac
      181 agtacttggt atgaatttat ctcatggtgg tcacttaaca cacggaagcc ctgttaactt
      241 cagtggagta caatataatt tcgtagaata tggcgtggat gctgactctc accgtattaa
      301 ttacgatgat gtattagcaa aagcgaaaga acataaacca aaattaatcg ttgcaggtgc
      361 aagtgcatac cctcgtgtta tcgatttcaa gcgattccgt gagattgcag atgaagtggg
      421 cgcttattta atggttgata tggcacatat cgctggttta gtagctgctg gtttacatcc
      481 aaatccagta ccacatgcac atttcgttac aacgacaaca cataaaacgt tacgtggccc
      541 gcgtggtggt atgattttat gtgaagagca atttgcaaaa caaattgata aatcaatctt
      601 ccctggtatt caaggtggtc cacttatgca cgtaatcgct gcaaaagctg ttgcgtttgg
      661 tgaagcactt caagatgatt tcaaaacata tgcacaaaat atcattaaca atgcgaaccg
      721 cttagctgaa ggtcttcaaa aagaaggact tacacttgtt tctggcggaa cagacaatca
      781 cttaatcttg attgatgttc gtaacttaga aatcacaggt aaagtagcag agcacgtatt
      841 agatgaagtt ggtattacag tgaacaaaaa tacaattcca tttgaaacag caagcccatt
      901 tgtaacaagt ggtgtacgta tcggtacagc agctgtaaca tctcgtggtt tcggtttaga
      961 agaaatggat gaaattgcgt cacttattgc ttatacatta aaaaatcatg aaaatgaagc
     1021 tgcattagaa gaagtacgta agcgtgtaga agcgttaact agcaaatttc caatgtatcc
     1081 aaatctataa tagattgaag aagactgccg agacttaatt gttttggcgg tcttttttgt
     1141 ggacatatat tatttttaaa gtatgtatac aaatgatgaa taaattttgg cgatataatg
     1201 aaggatacag ctcccataat tggtaaagat actagataga ttcatcgtaa aatcatgatt
     1261 ttgccaaatt tgcccttgaa tattagtagc gttttcttta caatcgtaaa tagtgtaaaa
     1321 aagcgtgcaa acgcatgaat atcatctaaa ggagagattc acatgggaaa actgtatgta
     1381 tttgatcctc tagaaggaaa attaatgtca gaacttatca aggaaaatat gcacatgaaa
     1441 ttgtacatgg aaggaacagt aaataatcac cactttaaat gtacctcaga aggagaagga
     1501 aaaccatatg aaggtactca aaccatgcgt attaaggccg ttgaaggtgg accattgcct
     1561 tttgcctttg atattcttgc tacatctttt atgtacggat caaagacttt tatcaatcat
     1621 acccaaggta tcccagattt ctttaaacag tcatttcctg aaggatttac atgggaacgt
     1681 gtcacaactt atgaagatgg tggagtattg acagcaactc aagatacatc tttgcaagat
     1741 ggttgtctta tctacaatgt aaagatccgt ggagttaatt ttccaagtaa tggtcctgtt
     1801 atgcagaaaa agacccttgg atgggaagca tctaccgaaa cattatatcc tgccgatggt
     1861 ggattggaag gtcgtgctga tatggcattg aaacttgtcg gtggaggtca ccttatctgt
     1921 aatttgaaga ccacataccg ttctaaaaag ccagctaaaa atcttaagat gcctggtgtt
     1981 tactacgtcg atcgtcgttt agaacgtatc aaagaagcat ataaggaaac ttatgttgaa
     2041 cagcacgaag tagccgtcgc acggtattgt gatttgccta gtaaattggg acaccgttaa
     2101 taataaaagc ttgagaaggc atgcaagctt gagtaggaca aatccgccga gcttcgacga
     2161 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     2221 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     2281 cctataacca gaccgttcag aacaaagaat acaagaaaat atttacaaaa aatcaattta
     2341 acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt gaacaattct
     2401 tatctctttt caatagctat aaattattta ataagtaagt taagggatgc ataaactgca
     2461 tcccttaact tgtttttcgt gtgcctattt tttgtgaatc gctaagaaac cattattatc
     2521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     2581 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2641 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2701 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tacaaaacat
     2761 atttcaacac aatacaaatg ggttagttaa aaaagcaggc cttctaaagg tctgcttttt
     2821 ttatttgatt atgtaatttt taatgccagg atgccaataa gccataacct caaatgcacc
     2881 atttgcaacc tcgtcatctt cttcctcaat cttgaccaga tcgccgtcct ccgcatcacc
     2941 aagattcagc tctttatgta tctccttcaa aatgccaccg tatccaatta accttcgagc
     3001 tgccaacgca tcatccaagt aaagcaccgt gttcagattg tcttcagtca ccttattacc
     3061 gcgcacaaca tccgtatcct taaccggata tttagagatt tcgagaacag ctttttgctc
     3121 catcattgcg ttccgcacat cgttttcaat ctgttcagcg tcaatcttag ctttaccttt
     3181 cactcgacga atatcgacaa ttggagtgta atccaatttc atcgcctttt tccaaaggct
     3241 cgtccactcc gcctgcttaa tatagttttt cccaaaataa tttttcctta ctggtatcaa
     3301 cacatgaaaa tgaggatgat atgtatcttc ttcatgattt ttggtaatct ctaaagctct
     3361 gaaaaatcca agaaccgaag tttttacttt tttgtactgg aacagtttcc taaagccttc
     3421 catcatcgca gaaatttgtg gcttcagccg ttctcccttt acatttcgaa tcgtcagcgt
     3481 gagaaaaatc catccgcagc cgtactgtct attggcttcc tctacgatca acttattgtg
     3541 ataagcaatt tttaacgacc tgcgccacgc acacatcgga cataacctca ctttacaaaa
     3601 atgggcttga tacagtttta acttgcccgt ctccgggtct ctcttaaacg aaagatactc
     3661 tgcacaacta attagttttt cagccttttt gccatagtaa ggtgccccaa tcttactctc
     3721 taacgcttcg taatgctccg ccatgaggtt cgtccgtctc tttttcccct tccaatcccg
     3781 ctttttacct gttgcggttt tatcttcgag gatgctataa tcattttcag atgaataaat
     3841 caacaaaaaa actccttctg agctagttct ctagcattct attattttga ttcgacacct
     3901 taataatagc agaaggagtt tttacctgtc aaagaaccat caaacccttg atacacaagg
     3961 ctttgaccta attttgaaaa atgatgttgt ttctatatag tatcaagata agaaagaaaa
     4021 ggatttttcg ctacgctcaa atcctttaaa aaaacacaaa agaccacatt ttttaatgtg
     4081 gtcttttatt cttcaactaa agcacccatt agttcaacaa acgaaaattg gataaagtgg
     4141 gatattttta aaatatatat ttatgttaca gtaatattga cttttaaaaa aggattgatt
     4201 ctaatgaaga aagcagacaa gtaagcctcc taaattcact ttagataaaa atttaggagg
     4261 catatcaaat gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta
     4321 atcattattt gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt
     4381 tataccgaaa cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga
     4441 caagggtgat aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag
     4501 gttattggga taagttagag ccactttata caatttttga tggtgtatct aaaacattct
     4561 ctggtatttg gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg
     4621 atgtagagaa atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg
     4681 ctttttctct ttctattatt ccatggactt catttactgg gtttaactta aatatcaata
     4741 ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt
     4801 caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag
     4861 gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg cttttataat
     4921 atgagataat gccgactgta ctttttacag tcggttttct aatgtcacta acctgccccg
     4981 ttagtcgcca ttcgccactg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     5161 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     5221 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5881 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     6541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6841 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7081 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7141 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7201 gaaaagtgcc acctgacgtc taagaaacca ttattatc
//
