LOCUS       sequence-261620-        5956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Wang S, El-Deiry WS
  TITLE     p73 or p53 directly regulates human p53 transcription to maintain 
            cell cycle checkpoints.
  JOURNAL   Cancer Res. 2006 Jul 15. 66(14):6982-9.
  PUBMED    16849542
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2006 Jul 15. 66(14):6982-9."
COMMENT     Sequence Label: pGL2-356bp
FEATURES             Location/Qualifiers
     source          1..5956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..2086
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(500..518)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2327..2392
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2522..2542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2967..3101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3243..3260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3414..4002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3494..3513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4173..4964,4965..5033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4796..4815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5034..5138)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5165..5620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5252..5271)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5462..5483
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5810..5931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgatccag ctgagagcaa acgcaaaagc tttcttcctt ccacccttca tatttgacac
       61 aatgcaggat tcctccaaaa tgatttccac caattctgcc ctcacagctc tggcttgcag
      121 aattttccac cccaaaatgt tagtatctac ggcaccaggt cggcgagaat cctgactctg
      181 caccctcctc cccaactcca tttcctttgc ttcctccggc aggcggatta cttgccctta
      241 cttgtcatgg cgactgtcca gctttgtgcc aggagcctcg caggggttga tgggattggg
      301 gttttcccct cccatgtgct caagactggc gctaaaagtt ttgagcttct caaaagtcta
      361 ggggaggtac cgagctctta cgcgtgctag ctcgagatct aagtaagctt ggcattccgg
      421 tactgttggt aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
      481 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
      541 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
      601 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
      661 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
      721 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
      781 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
      841 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
      901 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
      961 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
     1021 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
     1081 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     1141 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     1201 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
     1261 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
     1321 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     1381 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     1441 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     1501 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     1561 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     1621 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     1681 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     1741 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     1801 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     1861 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     1921 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     1981 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     2041 agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt
     2101 cagcgatgac gaaattctta gctattgtaa tactgcgatg agtggcaggg cggggcgtaa
     2161 tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat
     2221 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
     2281 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     2341 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     2401 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     2461 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     2521 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     2581 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     2641 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     2701 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     2761 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     2821 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     2881 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2941 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3061 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtcgaccg atgcccttga
     3121 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     3181 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     3241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5161 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5221 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5281 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5341 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5401 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5461 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5521 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5581 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
     5641 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     5701 ccatgataag taagtaatat taaggtacgt ggaggtttta cttgctttaa aaaacctccc
     5761 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atggtactgt
     5941 aactgagcta acataa
//
