LOCUS       sequence-261621-        5671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Wang S, El-Deiry WS
  TITLE     p73 or p53 directly regulates human p53 transcription to maintain 
            cell cycle checkpoints.
  JOURNAL   Cancer Res. 2006 Jul 15. 66(14):6982-9.
  PUBMED    16849542
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2006 Jul 15. 66(14):6982-9."
COMMENT     Sequence Label: pGL2-100bp
FEATURES             Location/Qualifiers
     source          1..5671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             149..1801
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(215..233)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2042..2107
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2237..2257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2682..2816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2958..2975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3129..3717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3209..3228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3888..4679,4680..4748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4511..4530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4749..4853)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4880..5335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4967..4986)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5177..5198
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5525..5646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctcc tttgtgccag gagcctcgca ggggttgatg ggattggggt
       61 tttcccctcc catgtgctca agactggcgc taaaagtttt gagcttctca aaagtctaga
      121 agcttggcat tccggtactg ttggtaaaat ggaagacgcc aaaaacataa agaaaggccc
      181 ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa
      241 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat
      301 cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
      361 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
      421 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
      481 acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa
      541 ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat
      601 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
      661 tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac
      721 aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc
      781 gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat
      841 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
      901 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
      961 gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct
     1021 attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     1081 aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt
     1141 ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat
     1201 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     1261 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg
     1321 tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     1381 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     1441 cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc
     1501 cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg
     1561 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     1621 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     1681 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     1741 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta
     1801 aaatgtaact gtattcagcg atgacgaaat tcttagctat tgtaatactg cgatgagtgg
     1861 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac
     1921 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgccggatct ttgtgaagga
     1981 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     2041 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     2101 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     2161 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     2221 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     2281 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     2341 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     2401 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     2461 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     2521 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     2581 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2641 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2701 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2761 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgtc
     2821 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     2881 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2941 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3001 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3721 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3781 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3841 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3901 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3961 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4021 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4081 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4141 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4201 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4261 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4321 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4381 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4441 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4501 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4561 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4621 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4681 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4741 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4861 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5101 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5161 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat
     5341 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5401 cgccagccca agctaccatg ataagtaagt aatattaagg tacgtggagg ttttacttgc
     5461 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5521 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5641 atcttatggt actgtaactg agctaacata a
//
