LOCUS       sequence-261629-        9358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SPARK TF component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: CMV:beta-2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
FEATURES             Location/Qualifiers
     source          1..9358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4318..4338
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             4351..4374
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4375..4812
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4813..5154
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             5155..5196
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5215..5803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5268..5288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5686..5697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5875..6108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6186..6307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6223..6242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6277..6296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6347..6482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6502..6521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6503..6521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6531..6548)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6531..6547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6540..6562)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6689..7144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6776..6795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6986..7007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7170..7274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7275..7343,7344..8135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7493..7512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8306..8894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8795..8814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9048..9065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9182..9203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9218..9223,9224..9241,9242..9248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9256..9272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9261..9283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9280..9296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9280..9296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9315..9335
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9317..9335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc accatggggc aacccgggaa cggcagcgcc
     2641 ttcttgctgg cacccaatag aagccatgcg ccggaccacg acgtcacgca gcagagggac
     2701 gaggtgtggg tggtgggcat gggcatcgtc atgtctctca tcgtcctggc catcgtgttt
     2761 ggcaatgtgc tggtcatcac agccattgcc aagttcgagc gtctgcagac ggtcaccaac
     2821 tacttcatca cttcactggc ctgtgctgat ctggtcatgg gcctggcagt ggtgcccttt
     2881 ggggccgccc atattcttat gaaaatgtgg acttttggca acttctggtg cgagttttgg
     2941 acttccattg atgtgctgtg cgtcacggcc agcattgaga ccctgtgcgt gatcgcagtg
     3001 gatcgctact ttgccattac ttcacctttc aagtaccaga gcctgctgac caagaataag
     3061 gcccgggtga tcattctgat ggtgtggatt gtgtcaggcc ttacctcctt cttgcccatt
     3121 cagatgcact ggtaccgggc cacccaccag gaagccatca actgctatgc caatgagacc
     3181 tgctgtgact tcttcacgaa ccaagcctat gccattgcct cttccatcgt gtccttctac
     3241 gttcccctgg tgatcatggt cttcgtctac tccagggtct ttcaggaggc caaaaggcag
     3301 ctccagaaga ttgacaaatc tgagggccgc ttccatgtcc agaaccttag ccaggtggag
     3361 caggatgggc ggacggggca tggactccgc agatcttcca agttctgctt gaaggagcac
     3421 aaagccctca agacgttagg catcatcatg ggcactttca ccctctgctg gctgcccttc
     3481 ttcatcgtta acattgtgca tgtgatccag gataacctca tccgtaagga agtttacatc
     3541 ctcctaaatt ggataggcta tgtcaattct ggtttcaatc cccttatcta ctgccggagc
     3601 ccagatttca ggattgcctt ccaggagctt ctgtgcctgc gcaggtcttc tttgaaggcc
     3661 tatgggaatg gctactccag caacggcaac acaggggagc agagtggata tcacgtggaa
     3721 caggagaaag aaaataaact gctgtgtgaa gacctcccag gcacggaaga ctttgtgggc
     3781 catcaaggta ctgtgcctag cgataacatt gattcacaag ggaggaattg tagtacaaat
     3841 gactcactgc tggagctggc cgagaagctg gccggcctgg acatcaacgg tggagctagc
     3901 gagtttcggg caaccacact ggaacggatc gagaaaagct tcgtgattac tgatccgaga
     3961 ctgcctgaca acccaatcat ttttgtgagc gattccttcc tgcagctgac agaatattct
     4021 cgggaagaga tcctggggcg caattgccgt tttctgcagg gacccgagac agaccgtgcc
     4081 actgttcgga aaatcagaga tgctattgac aaccagactg aagtgaccgt tcagctgatc
     4141 aattatacca agagcggcaa gaagttctgg aacgtgttcc acctgcagcc gatgcgcgat
     4201 tataagggcg acgtccagta cttcattggc gtgcagctgg atggcaccga acgtcttcat
     4261 ggcgccgctg agcgtgaggc ggtctgcctg gtcaaaaaga cagcctttca gattgctgag
     4321 aacctgtact tccagatggg cggaggatcc gactacaagg atgacgacga taagaagcta
     4381 ctgtcttcta tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa
     4441 gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa
     4501 accaaaaggt ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga
     4561 ctggaacagc tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg
     4621 gattctttac aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat
     4681 aaagatgccg tcacagatag attggcttca gtggagactg atatgcctct aacattgaga
     4741 cagcatagaa taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag
     4801 ttgactgtat cggccaattt taatcaaagt gggaatattg ctgatagctc attgtccttc
     4861 actttcacta acagtagcaa cggtccgaac ctcataacaa ctcaaacaaa ttctcaagcg
     4921 ctttcacaac caattgcctc ctctaacgtt catgataact tcatgaataa tgaaatcacg
     4981 gctagtaaaa ttgatgatgg taataattca aaaccactgt cacctggttg gacggaccaa
     5041 actgcgtata acgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat
     5101 gtatataact atctattcga tgatgaagat accccaccaa acccaaaaaa agagggcaag
     5161 ccgatcccta accctctgct gggcctggac agcacttaga cgcgttaagt cgacaatcaa
     5221 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5281 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5341 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5401 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5461 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5521 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5581 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5641 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5701 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5761 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     5821 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5881 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5941 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6001 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6061 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     6121 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6181 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6301 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6361 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6421 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6481 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     6541 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6601 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6661 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     6721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7141 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7501 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7981 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9301 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
