LOCUS       sequence-261649-       10003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Swap out effector and terminator to generate SPARC2-S CRISPR-donor 
            vector for CRISPR-HDR genomic insertion near the attP40 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE,
            Ishida IG, Maimon G, Wilson RI, Clandinin TR
  TITLE     SPARC enables genetic manipulation of precise proportions of cells.
  JOURNAL   Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 
            10.1038/s41593-020-0668-9. Epub 2020 Jul 20.
  PUBMED    32690967
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-0668-9"; journalName: "Nat Neurosci"; date: 
            "2020-09"; volume: "23"; issue: "9"; pages: "1168-1175"
COMMENT     Sequence Label: pHD-SPARC2-S-LexA::p65
FEATURES             Location/Qualifiers
     source          1..10003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1297..1391
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    1425..1519
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    1553..1647
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    1681..1775
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1800..2038
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1985..2006
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          2247..2312
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2442..2462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2734..2868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3271..3340
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(3347..3598,3599..3952)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             4397..4768
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     protein_bind    5072..5105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5331..5340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5337..6014
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5467..5485)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5517..5537)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5735..5754
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5929..5952
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    6135..6256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6263..6296
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7955..7972)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7955..7971)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8070..8087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8241..8829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8321..8340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8951..9742,9743..9811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9574..9593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9812..9903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9945..9964
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9970..9989
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
       61 atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga
      121 aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttcgc
      181 acagaaaagc atcttacgga tggtgtttgc caaaagtgct ggagatgttt tagatataga
      241 tggtttattt taagaaatgg atgtatataa ttgagctact gcatcacggg tttctgttta
      301 acagtaacag tgaaaagtgc tcaatgatac atatattaca aataataact tttggttttt
      361 aaagtagatt tatataatat aatcataatt cttaatggga atccatgtta cgaccaaatt
      421 agccgtggcc tttcaagtgg ctgccactta tattttacaa ctcgcacaca gtggctcaca
      481 ccctcgtaaa caaaataatg ctattaaatc aaattgaaca tgtgaattct ctcaagaaga
      541 cgccactttt ttttgggggg ccagctgcaa ctgccaccaa tgtttacttt tatctttaat
      601 gggcacatgc cttacatata cgaattttac acgatttttc aagtggcggt cgagagtggg
      661 gtgataatta attaaggttg cctttagata aagtattata gagtttcaaa aaaatgaaac
      721 ttaagaatgg aatagctgca ttccattcta tgtagatttc ccttcaaaat tattaaaatc
      781 cgttgacctt tgttcattta gcaattagaa ttcgtctatg aataacaaat taaattgcgt
      841 tgcgggcaaa ttgctttagg ccatttgaag tgcaacttta tatctcaggg ccaacttctc
      901 ggcgcgacaa caataaaaac atatacatag atacacatat cagatgcaat gcaatcacca
      961 cagcgtgacg tcattagtct aggcttgaaa atacacggca aacttgttta taactctacg
     1021 ctgattacat aagccgcaaa cacgacgaaa aaacatttgt tttggtttca aatccatgaa
     1081 tgactttcat taagtgcagc tgtaagctga gctttgccat gcgctgaccc atattttcag
     1141 cctctcgttc tctctgtttc gcagatactg tgaatattag taaaacgatc cataggggct
     1201 gtagtttata atcataatca aataggcata aacaaccacc agcgcggccg cggacatatg
     1261 ggatccagtg caccgtaagc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag
     1321 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     1381 tgtcctccga gcggagactc tagccctagg gcatgcctgc aggtcggagt actgtcctcc
     1441 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     1501 cggagtactg tcctccgagc ggagactcta gcgctagcgc atgcctgcag gtcggagtac
     1561 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     1621 cctccgagcg gagtactgtc ctccgagcgg agactctagc actagtgcat gcctgcaggt
     1681 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     1741 agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagcga cgtcgagcgc
     1801 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
     1861 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
     1921 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
     1981 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa
     2041 ttaacgtaag ctagctagag gatctttgtg aaggaacctt acggtaccaa tcaaaagatc
     2101 tactccagtc caactggggt aacctttgag ttctctcagt tggtaatacg taagctagct
     2161 agaggatctt tgtgaaggaa ccttacttgg taccctgtgg tgtgacataa ttggacaaac
     2221 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     2281 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     2341 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     2401 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     2461 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     2521 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     2581 acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc
     2641 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2701 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2821 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggc
     2881 gggagtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc gtagggtcgg
     2941 ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgtcg
     3001 tccactcgga tggctaaggg agagctcgga tccgggcaat acgaggtact ggcagacacg
     3061 cagtgtcgaa caacacctga cctaacggta agatgagtag ataactcaac tacttaaatc
     3121 ctggcctgct tgatatctcg tagcgttctt agagatcgac gaactgtttc acgaccgggt
     3181 cggcatggca tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc
     3241 actcggatgg ctaagggaga gctcggatcc ctcgaagccg cggtgcgggt gccagggcgt
     3301 gcccttgggc tccccgggcg cgtactccac ctcacccatc gtcgacatga aggctctcac
     3361 ggcccgacaa caggaagttt ttgatttgat acgggatcat atatcccaaa cgggtatgcc
     3421 tccgacccgc gcagagatag cacagcgact gggctttcga tcgcctaacg ccgcggagga
     3481 gcacttgaag gcactggccc gcaagggtgt cattgaaatc gtgtccggtg cgagccgcgg
     3541 aatccggctg ttgcaggagg aggaagaggg cctgccactg gtgggacgcg tggccgctgg
     3601 cgagccgctg ctggcccagc aacacataga gggacactat caggtggacc cctccttgtt
     3661 taagccaaat gctgatttcc tgttgcgggt gtcgggaatg tccatgaagg acatcggtat
     3721 tatggatggt gacctcctcg ccgtccataa gacacaggat gtcaggaacg gccaggtagt
     3781 cgttgccagg atagacgatg aggtcactgt gaaacgtctc aagaagcaag gcaataaggt
     3841 cgagctgctg ccggagaata gcgagttcaa gccgatcgtg gtggatctgc gacagcagtc
     3901 ctttactatc gagggcttgg ccgtgggtgt gatccgcaac ggagattggc tgggatccac
     3961 gccgatggag ttccagtacc tgcccgatac ggatgaccgt caccgtatcg aagaaaagcg
     4021 gaagcgaacc tatgaaacct tcaagtccat catgaaaaag tcccccttct cgggccccac
     4081 ggacccgcgc cccccgcccc gtcgtattgc ggttccttcg cgcagcagtg ccagcgtccc
     4141 caaacccgca ccgcagccct acccgttcac ttcctccctt agcacgatta actatgatga
     4201 gttccccacg atggtgttcc ccagtggaca aatttcccag gcatcggcac tggctccggc
     4261 cccaccgcaa gtcctccccc aggcgcccgc tccggcaccg gctcccgcaa tggtgagtgc
     4321 tctggcccag gcccccgctc cagtccccgt gctggcgcct ggacccccac aggcagttgc
     4381 ccctcctgct ccgaaaccaa cgcaggcggg cgaaggaacc ctgagcgagg ccctcttgca
     4441 gcttcagttc gatgacgaag acttgggagc cctgctgggt aacagcacag accctgccgt
     4501 attcaccgat ctcgcatccg tggacaacag cgagtttcag cagctcttga atcagggaat
     4561 cccggtcgca cctcatacca cagagcccat gctgatggaa tacccggagg ctatcacgcg
     4621 actggtgacc ggcgcacagc gaccacccga tccagcccct gccccactgg gtgccccggg
     4681 tttgcccaat ggcctcctca gcggcgatga ggatttctcc agcatcgctg atatggattt
     4741 ctccgctttg ctgagccaga taagctccta agtttaaaca agcttatcga taccgtcgac
     4801 taaagccaaa tagaaaatta ttcagttcct ggcttaagtt tttaaaagtg atattattta
     4861 tttggttgta accaaccaaa agaatgtaaa taactaatac ataattatgt tagttttaag
     4921 ttagcaacaa attgatttta gctatattag ctacttggtt aataaataga atatatttat
     4981 ttaaagataa ttgcgttttt attgtcaggg agtgagtttg cttaaaaact cgtttagatc
     5041 cactaggtcg actgaggcac tgcctcgaga gataacttcg tataatgtat gctatacgaa
     5101 gttatcgtac gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg
     5161 atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct
     5221 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     5281 aaataaacaa gcgcagctga acaagctaaa caatcggctc gaagccggtc gccaccatgg
     5341 cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
     5401 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     5461 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     5521 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     5581 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     5641 gcggcgtggt gaccgtgacc caggactcct ccctccagga cggctccttc atctacaagg
     5701 tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg
     5761 gctgggaggc gtccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     5821 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
     5881 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca
     5941 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc
     6001 acctgttcct gtaggggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     6061 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6121 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6241 tcatcaatgt atcttaaccg gtataacttc gtataatgta tgctatacga agttatcaca
     6301 ttgggtagca cagactctga gctttactac gattactata cagctcttct ctcgcgactt
     6361 tttggactgg acaaggcgta gcacattgaa cggcagtggg tttgggttta gtatcgaacc
     6421 ggctttctac gacagcggat tggaagcgcg gagcgacaaa gtcgccgggt aataatgtga
     6481 tatagccggc tatgttcagc aggcaaaact gaaataaaag taataaacac cgaaagcccc
     6541 cgaattctcg catcttcttt tgtgggactg cgagaagaat tgcgagcgaa atactaattt
     6601 ccttacctaa ctgtgtgaga taatctccgg ttggcattgt gtgtcgcgca ataaaatccc
     6661 caaaagccca aagcttcaag tgtttaatat aataaaatag ccaagaagcg tgtgtatttg
     6721 catttgggaa aaatttaagg ttgaattacg tgggccgaaa aattaaagac tgaaggccat
     6781 tcaagggttg ttcaaagaga aaattcggtg aaaagtcacc gtgtgtgtgt gtgtgagtgt
     6841 gtgtgcgttg cgtgagaaaa atcaattctg catttggtgg aaagccgaaa acggagagaa
     6901 aattggtgga gcgaggagaa aaggacacga attaaaggct ggttttttgg ggctgcgaaa
     6961 aactcgtgac cgatgacaat ttggcttgac gagggtctat tagcgaaatc aatgctatta
     7021 tggctgatag tggaggacca ggatccaagc acatggatcc aacaaccata gatgccggcg
     7081 ggggcggagc aggagcagcc ggaggtgatg acgtgcctcc agtcccagct gttcgccggc
     7141 gtcgtgcgca tgagcagaaa tcctcgagag agcaggtgct ggaggaggag aagagccaac
     7201 agctggagac atcgacgatg acgaggacct acatgaagac catcaccacc tccctcacca
     7261 cctcgagtag ctccaacgtg gaagagttta tcctggggga gcacgcagca ggagcagcag
     7321 ctgctccatc gcccaaccag cagcgattgc gtcaaaaggt ggcccagtac gagaaggtct
     7381 ggtcggatgg ctccagtccc gttaagcgtc cagccgagca gagctctgat gagctgcgtc
     7441 tgaccgacga ccaggatgag tacgccatcc gtaagatgct tttctgtgtc aatcgagttc
     7501 aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg
     7561 tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct
     7621 ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata
     7681 caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc
     7741 aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat
     7801 atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc
     7861 cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc
     7921 gttatcagaa ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa
     7981 agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct
     8041 ggcagttccc tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8641 gagttcttga agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc
     8701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     8881 cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt
     8941 aatgctctgc ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9061 catctggccc cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat
     9121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9241 gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9781 taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca
     9841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9901 aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta
     9961 tacctgaata tggctcataa caccccttgt ttgcctggcg gca
//
