LOCUS       sequence-26165-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-HA-CUL1 L756A/I757A
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(249..269)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        254..583
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      434..569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     496..515
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(705..724)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caactcgagg aagacgcaaa ctactgattc aggcggccat cgtgagaatc atgaagatga
       61 ggaaggttct gaaacaccag cagttacttg gcgaggtcct cactcagctg tcctccaggt
      121 tcaaacctcg agtccctgtg atcaagaaat gcattgacat tgctgcagag aaagaatatt
      181 tggagcgagt ggatggtgaa aaggacacct acagttactt ggcttaaccc ttctggatcc
      241 agatctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
      541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
      601 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
      661 agatggattg cacgcaggtt ctcccgccnc ctngggtgga nagnctattc ggctatgact
      721 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccgggctgtc agcgcagggg
      781 cgccnggttc tttttgtcag accgactgtc cggtgccntg atgaactgca ggacaag
//
