LOCUS       sequence-261651-        9783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Reinhard NR, Van Der Niet S, Chertkova A, Postma M, Hordijk PL, 
            Gadella TWJ Jr, Goedhart J
  TITLE     Identification of guanine nucleotide exchange factors that increase 
            Cdc42 activity in primary human endothelial cells.
  JOURNAL   Small GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509.
  PUBMED    31469028
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small 
            GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509."
COMMENT     Sequence Label: mCherry-ITSN2
FEATURES             Location/Qualifiers
     source          1..9783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6608..6627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6662..6681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6699..7154)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6836..6857)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7048..7067
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7181..7285
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7287..7644
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7287..7478
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7310..7330)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7495..7630
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7679..8473
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7679..8473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7733..7752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8343..8362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8661..8680)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8705..8752
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9081..9669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9570..9589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacgctca gtttcccaca
     1381 gctatgaatg gagggccaaa catgtgggct atcacctctg aagaacgtac taagcatgac
     1441 aggcagtttg ataacctcaa accttcagga ggttacataa caggtgatca agcacgtaat
     1501 tttttcctac aatcaggtct gccggcccct gttttagctg aaatatgggc tttatcagac
     1561 ctaaacaagg atgggaagat ggatcagcaa gagttctcca tagctatgaa actcatcaaa
     1621 ctgaagcttc aaggccaaca gttgcctgtg gttctccctc ctattatgaa gcaaccccct
     1681 atgttttctc cattaatttc tgctcgtttt ggaatgggaa gcatgcccaa tctgtccatt
     1741 cctcagccat tgcctccagc tgcacctata acatcattgt cttctgcgac ttcagggacc
     1801 aaccttcctc ccttaatgat gcccactccc ctagtgcctt ctgttagcac atcatcatta
     1861 ccaaatggaa ccgccagtct cattcagcct ttacccattc cttattcttc ttcaacattg
     1921 cctcatgggt catcttatag tctgatgatg ggaggatttg gaggtgctag tatacagaaa
     1981 gcgcagtctc tgattgattt aggatctagt agctcaactt cctcgactgc ttcactctca
     2041 gggaactcac ccaagactgg gacctcagag tgggcagttc ctcagcctac aagattaaaa
     2101 tatcggcaaa aatttaatac tcttgacaaa agtatgagtg gatatctctc aggttttcaa
     2161 gctagaaatg cccttcttca gtcaaatctt tctcaaactc agctggctac tatttggact
     2221 ctggctgacg ttgatggtga tggacagcta aaagcagaag agtttattct tgcaatgcac
     2281 cttactgaca tggccaaagc tggacagcca ttaccactga ctttacctcc tgagcttgtt
     2341 cctccatctt tcagaggagg aaagcaaatt gattccatta atggaactct gccttcatat
     2401 cagaaaatgc aagaagagga gcctcagaag aaattaccag ttacttttga ggacaaacgg
     2461 aaagccaact atgagcgagg gaacatggag ctggaaaagc gacgccaagc cttgatggag
     2521 cagcaacaaa gggaggcaga acgtaaagcc cagaaagaaa aggaagagtg ggaacgaaaa
     2581 cagagagaat tacaagaaca agaatggaag aaacaacttg aattagaaaa acgcttagag
     2641 aagcaacggg aattggagag acaacgagag gaagaaagga gaaaagacat agaaagacga
     2701 gaggcagcaa aacaggaact tgaacgacaa cgtcgcttag aatgggagag aattcggcga
     2761 caggagcttc tcaatcaaaa ggatagagaa caagaagaaa ttgtcaggtt aaactctaaa
     2821 aagaagaatc ttcatcttga gttggaagca ctgaatggca aacatcagca gatctcaggc
     2881 agacttcagg atgtccgact caaaaaacaa actcaaaaga ctgagctgga agttctggat
     2941 aagcagtgtg acttggaaat tatggaaatc aagcaacttc aacaggaact tcaggaatat
     3001 cagaataagc ttatctatct ggtacctgag aagcaattat taaatgaaag aattaaaaac
     3061 atgcagttca gtaacacacc tgattcaggg gtcagtttac ttcataaaaa atcattagaa
     3121 aaggaagaat tatgccaaag acttaaagaa cagttagatg ctcttgaaaa agaaactgca
     3181 tctaagctgt cagaaatgga ttcttttaac aatcaactaa agtgtgggaa tatggatgac
     3241 tctgttcttc agtgcctttt gtctctgcta agctgtctca acaacctctt cctcttactt
     3301 aaggaactga gagaaaccta caacacacag cagttagccc ttgaacagct ttataagatc
     3361 aaacgtgaca agttgaagga aattgaaagg aaaagattag aactaatgca gaaaaagaaa
     3421 ctagaagatg aggctgcaag gaaagcaaag caaggaaaag aaaacttatg gaaagaaaat
     3481 cttagaaagg aggaagaaga aaaacaaaag cgactccagg aagaaaaaac acaagaaaaa
     3541 attcaagaag aggaacggaa agctgaggag aaacaacgta aggataagga tactttgaaa
     3601 gctgaggaga aaaaacgtga gacagctagt gttttggtga attatagagc attatacccc
     3661 tttgaagcaa ggaaccatga tgagatgagt tttaattctg gagatataat tcaggttgat
     3721 gaaaaaaccg taggagagcc tggttggctt tatggtagtt ttcaaggaaa ttttggctgg
     3781 tttccatgca attatgtaga aaaaatgcca tcaagtgaaa atgaaaaagc tgtatctcca
     3841 aagaaggcct tacttcctcc tacagtttct ttatctgcta cctcaacttc ctctgaacca
     3901 ctttcttcaa atcaaccagc atcagtgact gattatcaaa atgtatcttt ttcaaaccta
     3961 actgtaaata catcatggca gaaaaaatca gccttcactc gaactgtgtc ccctggatct
     4021 gtatcaccta ttcatggaca gggacaagtg gtagaaaact taaaagcaca ggccctttgt
     4081 tcctggactg caaagaaaga taaccacttg aacttctcaa aacatgacat tattactgtc
     4141 ttggagcagc aagaaaattg gtggtttggg gaggtgcatg gaggaagagg atggtttccc
     4201 aaatcttatg tcaagatcat tcctgggagt gaagtaaaac gggaagaacc agaagctttg
     4261 tatgcagctg taaataagaa acctacctcg gcagcctatt cagttggaga agaatatatt
     4321 gcactttatc catattcaag tgtggaacct ggagatttga ctttcacaga aggtgaagaa
     4381 atattggtga cccagaaaga tggagagtgg tggacaggaa gtattggaga tagaagtgga
     4441 atttttccat caaactatgt caaaccaaag gatcaagaga gttttgggag tgctagcaag
     4501 tctggagcat caaataaaaa acctgagatt gctcaggtaa cttcagcata tgttgcttct
     4561 ggttctgaac aacttagcct tgcaccagga cagttaatat taattctaaa gaaaaataca
     4621 agtgggtggt ggcaaggaga gttacaggcc agaggaaaaa agcgacagaa aggatggttt
     4681 cctgccagtc atgttaaact tttgggtcca agtagtgaaa gagccacacc tgcctttcat
     4741 cctgtatgtc aggtgattgc tatgtatgac tatgcagcaa ataatgaaga tgagctcagt
     4801 ttctccaagg gacaactcat taatgttatg aacaaagatg atcctgattg gtggcaagga
     4861 gagatcaacg gggtgactgg tctctttcct tcaaactacg ttaagatgac gacagactca
     4921 gatccaagtc aacagtggtg tgctgatctg caaaccctgg acacaatgca gccaattgag
     4981 aggaaaagac agggctatat tcatgagctg attcagaccg aagagcggta catggctgac
     5041 cttcagctcg tcgtcgaggt ttttcagaaa cgcatggcag agtcaggctt tctcactgaa
     5101 ggggaggtgg ccctgatttt tgttaactgg aaggagctca tcatgtccaa cacaaagctg
     5161 ctgaaggctt tgcgggtgcg gaagaagacc gggggcgaga agatgccggt gcagatgatt
     5221 ggggacatcc tggccgctga gctgtcccac atgcaggctt acatcaggtt ctgcagctgc
     5281 cagcttaatg gagcagctct gttacagcag aagacagatg aagacacaga tttcaaagaa
     5341 tttttaaaga agctggcatc tgacccgcgg tgtaaaggaa tgcccctctc cagcttcctg
     5401 ctgaaaccca tgcagaggat cacccgctac ccactgctca tcagaagtat tctggagaac
     5461 accccggaga gccatgcaga ccattcctcc ctaaagctgg ccctcgagcg ggcagaggag
     5521 ctgtgctctc aagtgaatga gggagttcgg gagaaggaaa actcggaccg actggagtgg
     5581 atccaggcgc acgtgcagtg tgaaggcctc gcggagcaac ttattttcaa ctctctcacc
     5641 aactgcctgg ggccccggaa gctcttacac agtgggaaat tatacaagac caagagcaac
     5701 aaggaactgc acggattcct cttcaatgac ttcctgcttc ttacctacat ggtcaagcag
     5761 tttgctgttt cctctggctc tgagaaactt ttcagctcga agtccaatgc tcaattcaaa
     5821 atgtataaaa cgcccatttt cctgaatgaa gtcttggtga aactgcccac agacccttcc
     5881 agcgatgagc ctgtcttcca catttcccac attgatcggg tctacaccct ccgaacagac
     5941 aacattaatg agaggaccgc ctgggtgcag aagatcaagg cggcgtctga gcagtacatc
     6001 gacaccgaga agaagaagcg tgagaaagct taccaagccc gctcccaaaa gacttcaggc
     6061 attgggcgcc tgatggtgca tgtcattgaa gctacagaat taaaagcctg caaaccaaat
     6121 ggaaagagca acccatactg tgaaatcagc atgggctccc agagctacac caccaggacc
     6181 gtccaggaca cactcaatcc caagtggaat tttaactgcc agttctttat taaggatctc
     6241 taccaagacg tgctgtgtct caccctgttt gacagagacc agttttcacc agatgatttc
     6301 ctgggtcgta ctgaaattcc agtggcaaaa attcgaacag aacaggaaag caaaggccct
     6361 atgacccgcc gactgctgct gcatgaggtc cccaccgggg aggtctgggt ccgttttgac
     6421 ctgcagcttt ttgagcaaaa aactctcctg taatctagat aactgatcat aatcagccat
     6481 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     6541 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
     6721 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     6781 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6841 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6901 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6961 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     7021 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     7081 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     7141 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7261 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     7321 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7381 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7441 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7501 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7621 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7681 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7741 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7801 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7861 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7921 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7981 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8041 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8101 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8161 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     8221 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8281 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8341 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8401 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8461 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8521 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8581 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8641 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8701 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8761 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8821 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8881 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8941 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9781 cat
//
