LOCUS       sequence-261652-        9747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Reinhard NR, Van Der Niet S, Chertkova A, Postma M, Hordijk PL, 
            Gadella TWJ Jr, Goedhart J
  TITLE     Identification of guanine nucleotide exchange factors that increase 
            Cdc42 activity in primary human endothelial cells.
  JOURNAL   Small GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509.
  PUBMED    31469028
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small 
            GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509."
COMMENT     Sequence Label: mCherry-TrioN
FEATURES             Location/Qualifiers
     source          1..9747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6535..6656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6572..6591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6626..6645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6663..7118)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6800..6821)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7012..7031
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7145..7249
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7251..7608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7251..7442
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7274..7294)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7459..7594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7643..8437
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7643..8437
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7697..7716)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8307..8326
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8625..8644)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8669..8716
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9045..9633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9534..9553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggaatga aagctatgga tgttttacca attttgaagg aaaaagttgc atacctttca
     1381 ggtgggagag ataaacgtgg aggtcccatt ttaacgtttc cggcccgcag caatcatgac
     1441 agaatacgac aggaggatct caggagactc atttcctatc tagcctgtat tcccagcgag
     1501 gaggtctgca agcgtggctt cacggtgatc gtggacatgc gtgggtccaa gtgggactcc
     1561 atcaagcccc ttctgaagat cctgcaggag tccttcccct gctgcatcca tgtggccctg
     1621 atcatcaagc cagacaactt ctggcagaaa cagaggacta attttggcag ttctaaattt
     1681 gaatttgaga caaatatggt ctctttagaa ggccttacca aagtagttga tccttctcag
     1741 ctaactcctg agtttgatgg ctgcctggaa tacaaccacg aagaatggat tgaaatcaga
     1801 gttgcttttg aagactacat tagcaatgcc acccacatgc tgtctcggct ggaggaactt
     1861 caggacatcc tagctaagaa ggagctgcct caggatttag agggggctcg gaatatgatc
     1921 gaggaacatt ctcagctgaa gaagaaggtg attaaggccc ccatcgagga cctggatttg
     1981 gagggacaga agctgcttca gaggatacag agcagtgaaa gctttcccaa aaagaactca
     2041 ggctcaggca atgcggacct gcagaacctc ttgcccaagg tgtccaccat gctggaccgg
     2101 ctgcactcga cacggcagca tctgcaccag atgtggcatg tgaggaagct gaagctggac
     2161 cagtgcttcc agctgaggct gtttgaacag gatgctgaga agatgtttga ctggatcaca
     2221 cacaacaaag gcctgtttct aaacagctac acagagattg ggaccagcca ccctcatgcc
     2281 atggagcttc agacgcagca caatcacttt gccatgaact gtatgaacgt gtatgtaaat
     2341 ataaaccgca tcatgtcggt ggccaatcgt ctggtggagt ctggccacta tgcctcgcag
     2401 cagatcaggc agatcgcgag tcagctggag caggagtgga aggcgtttgc ggcagccctg
     2461 gatgagcgga gcaccttgct ggacatgtcc tccattttcc accagaaggc cgaaaagtat
     2521 atgagcaacg tggattcatg gtgtaaagct tgcggtgagg tagaccttcc ctcagagctg
     2581 caggacctag aagatgccat tcatcaccac cagggaatat atgaacatat cactcttgct
     2641 tattctgagg tcagccaaga tgggaagtcg ctccttgaca agctccagcg gcccttgact
     2701 cccggcagct ccgattccct gacagcctct gccaactact ccaaggccgt gcaccatgtc
     2761 ctggatgtca tccacgaggt gctgcaccac cagcggcagc tggagaacat ctggcaacac
     2821 cgcaaggtcc ggctgcatca gaggctgcag ctgtgtgttt tccagcagga cgttcagcag
     2881 gtgctagact ggatcgagaa ccacggagaa gcatttctga gcaaacatac aggtgtgggg
     2941 aaatctcttc atcgggccag agcattgcag aaacgtcatg aagattttga agaagtggca
     3001 cagaacacat acaccaatgc ggataaatta ctggaagcag cagaacagct ggctcagact
     3061 ggggaatgtg accccgaaga gatttatcag gctgcccatc agctggaaga ccggattcaa
     3121 gatttcgttc ggcgtgttga gcagcgaaag atcctactgg acatgtcagt gtcctttcac
     3181 acccatgtga aagagctgtg gacgtggctg gaggagctgc agaaggagct gctggacgac
     3241 gtgtatgccg agtcggtgga ggccgtgcag gacctcatca agcgctttgg ccagcagcag
     3301 cagaccaccc tgcaggtgac tgtcaacgtg atcaaggaag gggaggacct catccagcag
     3361 ctcagggact ctgccatctc cagtaacaag accccccaca acagctccat caaccacatt
     3421 gagacggtgc tgcagcagct ggacgaggcg cagtcgcaga tggaggagct cttccaggag
     3481 cgcaagatca agctggagct cttcctgcag ctgcgcatct tcgagaggga cgccatcgac
     3541 attatctcag acctcgagtc ttggaatgat gagctttctc agcaaatgaa tgacttcgac
     3601 acagaagatc tcacgattgc agagcagcgc ctccagcacc atgcagacaa agccttgacc
     3661 atgaacaact tgacttttga cgtcatccac caagggcaag atcttctgca gtatgtcaat
     3721 gaggtccagg cctctggtgt ggagctgctg tgtgatagag atgtagacat ggcaactcgg
     3781 gtccaggacc tgctggagtt tcttcatgaa aaacagcagg aattggattt agccgcagag
     3841 cagcatcgga aacacctgga gcagtgcgtg cagctgcgcc acctgcaggc agaagtgaaa
     3901 caggtgctgg gttggatccg caacggagag tccatgttaa atgccggact tatcacagcc
     3961 agctcgttac aagaggcaga gcagctccag cgagagcacg agcagttcca gcatgccatt
     4021 gagaaaacac atcagagcgc gctgcaggtg cagcagaagg cagaagccat gctacaggcc
     4081 aaccactacg acatggacat gatccgggac tgcgccgaga aggtggcgtc tcactggcaa
     4141 cagctcatgc tcaagatgga agatcgcctc aagctcgtca atgcctctgt cgctttctac
     4201 aaaacctcag agcaggtctg cagcgtcctc gagagcctgg aacaggagta caagagagaa
     4261 gaagactggt gtggcggggc ggataagctg ggcccaaact ctgagacgga ccacgtgacg
     4321 cccatgatca gcaagcacct ggagcagaag gaggcattcc tgaaggcttg cacccttgct
     4381 cggaggaatg cagacgtctt cctgaaatac ctgcacagga acagcgtgaa catgccagga
     4441 atggtgacgc acatcaaagc tcctgaacag caagtgaaaa atatcttgaa tgaactcttc
     4501 caacgggaga acagggtatt gcattactgg accatgagga agagacggct ggaccagtgt
     4561 cagcagtacg tggtctttga gaggagtgcc aagcaggctt tggaatggat ccatgacaat
     4621 ggcgagttct acctttccac acacacctcc acgggctcca gtatacagca cacccaggag
     4681 ctcctgaaag agcacgagga gttccagata actgcaaagc aaaccaaaga gagagtgaag
     4741 ctattgatac agctggctga tggcttttgt gaaaaagggc atgcccatgc ggcagagata
     4801 aaaaaatgtg ttactgctgt ggataagagg tacagagatt tctctctgcg gatggagaag
     4861 tacaggacct ctttggaaaa agccctgggg atttcttcag attccaacaa atcgagtaaa
     4921 agtctccagc tagatatcat tccagccagt atccctggct cagaggtgaa acttcgagat
     4981 gctgctcatg aacttaatga agagaagcgg aaatctgccc gcaggaaaga gttcataatg
     5041 gctgagctca ttcaaactga aaaggcttat gtaagagacc tccgggaatg tatggatacg
     5101 tacctgtggg aaatgaccag tggcgtggaa gagattccac ctggcattgt aaacaaagaa
     5161 ctcatcatct tcggaaacat gcaagaaatc tacgaatttc ataataacat attcctaaag
     5221 gagctggaaa aatatgaaca gttgccagag gatgttggac attgttttgt tacttgggca
     5281 gacaagtttc agatgtatgt cacatattgc aaaaataagc ctgattctac tcagctgata
     5341 ttggaacatg cagggtccta ttttgacgag atacagcagc gacatggatt agccaattcc
     5401 atttcttcct accttattaa accagttcag cgaataacga agtatcagct ccttttaaaa
     5461 gagctgctga cgtgctgtga ggaaggaaag ggagagatta aagatggcct ggaggtgatg
     5521 ctcagcgtgc cgaagcgagc caatgacgcc atgcacctca gcatgctgga agggtttgat
     5581 gaaaacattg agtctcaggg agaactcatc ctacaggaat ccttccaagt gtgggaccca
     5641 aaaaccttaa ttcgaaaggg tcgagaacgg catctcttcc tttttgaaat gtccttagta
     5701 tttagtaaag aagtgaaaga ttccagtggg agaagcaagt acctttataa aagcaaattg
     5761 tttacctcag agttgggtgt cacagaacat gttgaaggag acccttgcaa atttgcactg
     5821 tgggtgggga gaacaccaac ttcagataat aaaattgtcc ttaaggcttc cagcatagag
     5881 aacaagcagg actggataaa gcatatccgc gaagtcatcc aggagcggac gatccacctg
     5941 aagggagccc tgaaggagcc cattcacatc cctaagaccg ctcccgccac aagacagaag
     6001 ggaaggaggg atggagagga tctggacagc caaggagacg gcagcagcca gcctgatacg
     6061 atttccatcg cctcacggac gtctcagaac acgctggaca gcgataagct ctctggtggc
     6121 tgtgagctga cagtggtgat ccatgacttc accgcttgca acagcaacga gctgaccatc
     6181 cgacggggcc agaccgtgga agttctggag cggccgcatg acaagcctga ctggtgtctg
     6241 gtgcggacca ctgaccgctc cccagcggca gaaggcctgg tcccctgtgg ttcactgtgc
     6301 atcgcccact ccagaagtag catggaaatg gagggcatct tcaaccacaa agactcgctc
     6361 tccgtctcca gcaatgacgc ctaggtcgac ggtaccgcgg gcccgggatc caccggatct
     6421 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6481 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     6661 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6721 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6781 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6841 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6901 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6961 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7021 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7081 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     7141 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7201 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     7261 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7321 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     7621 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     7681 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7741 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7801 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     7861 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7921 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7981 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8041 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8101 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8161 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8221 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8281 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8341 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8401 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     8461 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     8521 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8581 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     8641 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     8701 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     8761 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     8821 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     8881 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9721 gtggataacc gtattaccgc catgcat
//
