LOCUS       sequence-261656-        6135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6135)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmin-C-18
FEATURES             Location/Qualifiers
     source          1..6135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(208..227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     555..576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1939..1950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2212..2333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2249..2268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2303..2322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2340..2795)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2477..2498)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2689..2708
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2822..2926
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2928..3285
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2928..3119
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2951..2971)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3136..3271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3320..4114
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3320..4114
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3374..3393)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3984..4003
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4302..4321)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4346..4393
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4722..5310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5211..5230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5485..5788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5789..5992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5943..5963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6031..6040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6037..6039,6040..6042,6043..6135,1..618)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6082..6103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
       61 ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc
      121 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      181 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      241 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      301 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
      361 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
      421 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      481 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
      541 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      601 atggacgagc tgtacaagtc cggactcaga tctggcagcg gtggaggcag cgcatccggc
      661 ggaagcggaa gcagccaggc ctactcgtcc agccagcgcg tgtcctccta ccgccgcacc
      721 ttcggcgggg ccccgggctt cccgctcggc tccccgctga gctcgcccgt gttcccgcgg
      781 gcgggtttcg gctctaaggg ctcctccagc tcggtgacgt cccgcgtgta ccaggtgtcg
      841 cgcacgtcgg gcggggccgg gggcctgggg tcgctgcggg ccagccggct ggggaccacc
      901 cgcacgccct cctcctacgg cgcaggcgag ctgctggact tctcactggc cgacgcggtg
      961 aaccaggagt ttctgaccac gcgcaccaac gagaaggtgg agctgcagga gctcaatgac
     1021 cgcttcgcca actacatcga gaaggtgcgc ttcctggagc agcagaacgc ggcgctcgcc
     1081 gccgaagtga accggctcaa gggccgcgag ccgacgcgag tggccgagct ctacgaggag
     1141 gagctgcggg agctgcggcg ccaggtggag gtgctcacta accagcgcgc gcgcgtcgac
     1201 gtcgagcgcg acaacctgct cgacgacctg cagcggctca aggccaagct gcaggaggag
     1261 attcagttga aggaagaagc agagaacaat ttggctgcct tccgagcgga cgtggatgca
     1321 gctactctag ctcgcattga cctggagcgc agaattgaat ctctcaacga ggagatcgcg
     1381 ttccttaaga aagtgcatga agaggagatc cgtgagttgc aggctcagct tcaggaacag
     1441 caggtccagg tggagatgga catgtctaag ccagacctca ctgccgccct cagggatatc
     1501 cgggctcagt atgagaccat cgcggctaag aacatttctg aagctgagga gtggtacaag
     1561 tcgaaggtgt cagacctgac ccaggcagcc aacaagaaca acgacgccct gcgccaggcc
     1621 aagcaggaga tgatggaata ccgacaccag atccagtcct acacctgcga gattgacgcc
     1681 ctcaagggca ctaacgattc cctgatgagg cagatgcggg aattggagga ccgatttgcc
     1741 agtgaggcca gtggctacca ggacaacatt gcacgcctgg aggaggaaat ccggcacctc
     1801 aaggatgaga tggcccgcca tctgcgcgag taccaggacc tgctcaacgt gaagatggcc
     1861 ctggatgtgg agattgccac ctaccggaag ctgctggagg gagaggagag ccggatcaat
     1921 ctccccatcc agacctactc tgccctcaat ttccgagaaa ccagccctga gcaaaggggt
     1981 tctgaggtcc ataccaagaa gacggtgatg atcaagacca tcgagacacg ggatggggag
     2041 gtcgtcagtg aggccacaca gcagcagcat gaagtgctct aaggatccac cggatctaga
     2101 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2161 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     2341 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2401 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2461 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2521 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2581 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2641 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2701 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     2761 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     2941 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     3301 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3361 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3421 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3481 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3541 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3601 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     3661 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     3721 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     3781 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     3841 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     3901 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     3961 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4021 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4081 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4141 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4201 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4261 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     4321 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     4381 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     4441 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     4501 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     4561 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4621 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4681 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4741 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4801 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4861 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4921 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4981 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5041 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5101 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5161 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5221 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5281 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5341 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5401 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     5461 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     5521 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     5581 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     5641 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     5701 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5761 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     5821 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     5881 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     5941 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     6001 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     6061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6121 agcgtgtccg gcgag
//
