LOCUS       sequence-261666-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector of RGM-tdTomato with homologous arms around Mir290 
            super-enhancer DMR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Song Y, van den Berg PR, Markoulaki S, Soldner F, Dall'Agnese A, 
            Henninger JE, Drotar J, Rosenau N, Cohen MA, Young RA, Semrau S, 
            Stelzer Y, Jaenisch R
  TITLE     Dynamic Enhancer DNA Methylation as Basis for Transcriptional and 
            Cellular Heterogeneity of ESCs.
  JOURNAL   Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045.
  PUBMED    31422875
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045."
COMMENT     Sequence Label: miR290-SE-RGM-tdTomato targeting vector
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1183..2613
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2679..3267
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2732..2752)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3150..3161)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3270..3286)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3271..3287)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3288..3305)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3294..3501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3554..3587
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3648..4147
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3683..3706)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4080..4099
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4187..4786
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4187..4206)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    5343..5376
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6304..6323)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6305..6323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6330..6347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6330..6346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6339..6361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6487..6915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6574..6593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6784..6805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7259..8053
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7313..7332)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7923..7942
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8071..8139,8140..8931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8289..8308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9102..9690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9591..9610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9844..9861
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 cagggatact gtgtcttggg gagaaagctc tgttattaaa gcactactta ctttaacaag
      361 taaaaaacgt aacatttggg agccctcaaa cgcactgaca ttccaggtgg ccttgtcagc
      421 ctgcctgtaa ttcgggtttt taagtcaaag ttaaagcaag agagaaaaga tcccacccaa
      481 ggaacgcagt gatagaggat cgggagcgca gctcagggtt taaatcaagc acaacgtgct
      541 ttttcaaaat gtggtggtga atggcttatt cctgccactc gagacttaag ggcagggtgt
      601 ttctgtgagt tccaggcagg ccaggcctac atagtgagac caggtcacac acacatgcaa
      661 agcattcctt actgaaaaca atgaggggat ctcctggggc agaggcaggt gtgaaattct
      721 ggctgggtgg agtagaaggc ctctttatcg agtcagttta gactggactg aaccgggcca
      781 aaggagccga gcacatcaca cctccccgca ttatttcctg gaacctgggc aatctgaagg
      841 cagagaaagc tgcctggggc ccacagcccg gcactccttc ccgtatcaat tgacgctcaa
      901 atttccgcag taggaatgct caagcattcc ttttggtagc tgccttttgg caggacattc
      961 cggtcagagg gacagagacc cctgcattgc ggcaaaaatg tgcgcatgtg cagccattgc
     1021 ctgggacgca tgcgtaggga gccgcgcgac aaacctgagc cattgcggca agactagcgc
     1081 agagaggaga gggagccgga gatgccagac gcttggttct gaggagtgat ttgcaacgca
     1141 atggagcgag gaaggtcagc tgggcttgtg gattctgcta gcatggtgag caagggcgag
     1201 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     1261 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1321 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     1381 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     1441 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     1501 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     1561 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1621 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     1681 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     1741 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     1801 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     1861 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
     1921 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1981 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2041 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     2101 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     2161 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     2221 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     2281 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     2341 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     2401 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2461 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2521 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2581 ttcctgtacg gcatggacga gctgtacaag taagaattgt gttgcactta acgcgtacaa
     2641 ggccggccct gcaggaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     2701 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2761 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2821 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2881 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2941 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3001 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3061 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3121 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3181 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3241 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgacct cgactgtgcc
     3301 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3361 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3421 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3481 caatggcagg catgctgggg aactagtggt gccagggcgt gttaattaac gatcatattc
     3541 aataaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctgaagag
     3601 gagtttacgt ccagccaagc ttaggatctc gacctcgaaa ttctaccggg taggggaggc
     3661 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     3721 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     3781 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     3841 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     3901 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     3961 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     4021 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4081 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     4141 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
     4201 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4261 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4321 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4381 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4441 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     4501 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4561 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4621 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     4681 ccacaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     4741 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     4801 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
     4861 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
     4921 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
     4981 gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc
     5041 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
     5101 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
     5161 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
     5221 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
     5281 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagatcc ggaaccctta
     5341 atataacttc gtataatgta tgctatacga agttattagg tccctcgaag aggttcacta
     5401 ggcgcgcctt cccgtatcag ctctgaaatc tgcagagctg agatctggca ggaagcagga
     5461 gactcagtgg gaattctatc tgtctaaagt ctttgtgtga gtccagatct ctagatcctt
     5521 acgcaaagag taaagttagg aggactcctg acttcagggc tggtgggggc agggctggct
     5581 gccagcctga gagaacaaaa cgaagcaact gtgctgtagt ttgagtctgg tctgcaaggt
     5641 gactcctggt ataataccag cccttggaaa acagagtcct gagagagaaa aaagtaggag
     5701 aaagtttgaa gccagtttgg accacataaa aaatagactc tgtatctaaa acataactgg
     5761 ggccaggtgt ggtgataggc atctttaagc cagtatttga gagtcgaagg caggaggatg
     5821 tctgtgagtt caaggcctct cctgctctat atagcaattt caggacagcc tctactcgga
     5881 gaggctctct ctcaaaacca caataagcca ggtggtggtg gcacaggcct ttaatcccag
     5941 caccacttgg aaggcagagg cagaggcagg cggatttatg agtttgaggc cagcctgagt
     6001 gagttccagg acagccaggg ctacacagag aaaccctgtc tcaaaaaaaa caaaaaaaca
     6061 aaaaaaaaaa aaaaaccaat aactggttgg tgactcctgt ctcttattct agcacttaac
     6121 aggctgaggc aggagaatta ctgtaaatcc caaggcactc tgtgctagac agtaagacct
     6181 tgtctgaaag aaactgtata tagcaggcat agtggcaaat gccggccggc caataagggc
     6241 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat
     6301 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     6361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6481 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6601 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6661 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6721 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6781 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6841 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6901 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
     6961 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     7021 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     7081 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     7141 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     7201 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     7261 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7321 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7381 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7441 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7501 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7561 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7621 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7681 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7741 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7801 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7861 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7921 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7981 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8041 cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9721 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9781 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9841 cgcagcgagt cagtgagcga ggaagcggaa g
//
