LOCUS       sequence-261667-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector of RGM-eGFP with homologous arms around Mir290 
            super-enhancer DMR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Song Y, van den Berg PR, Markoulaki S, Soldner F, Dall'Agnese A, 
            Henninger JE, Drotar J, Rosenau N, Cohen MA, Young RA, Semrau S, 
            Stelzer Y, Jaenisch R
  TITLE     Dynamic Enhancer DNA Methylation as Basis for Transcriptional and 
            Cellular Heterogeneity of ESCs.
  JOURNAL   Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045.
  PUBMED    31422875
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045."
COMMENT     Sequence Label: miR290-SE-RGM-eGFP targeting vector
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1183..1185,1186..1188,1189..1902)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1183..1185,1186..1188,1189..1899)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1228..1249)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1489..1508)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1836..1857
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1992..2011)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2057..2112
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2111..2130)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    2473..2506
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2567..3066
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2602..2625)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2999..3018
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3106..3705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3106..3125)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4262..4295
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5223..5242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5224..5242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5249..5266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5249..5265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5258..5280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5406..5834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5493..5512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5703..5724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6178..6972
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6232..6251)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6842..6861
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6990..7058,7059..7850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7208..7227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8021..8609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8510..8529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8763..8780
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 cagggatact gtgtcttggg gagaaagctc tgttattaaa gcactactta ctttaacaag
      361 taaaaaacgt aacatttggg agccctcaaa cgcactgaca ttccaggtgg ccttgtcagc
      421 ctgcctgtaa ttcgggtttt taagtcaaag ttaaagcaag agagaaaaga tcccacccaa
      481 ggaacgcagt gatagaggat cgggagcgca gctcagggtt taaatcaagc acaacgtgct
      541 ttttcaaaat gtggtggtga atggcttatt cctgccactc gagacttaag ggcagggtgt
      601 ttctgtgagt tccaggcagg ccaggcctac atagtgagac caggtcacac acacatgcaa
      661 agcattcctt actgaaaaca atgaggggat ctcctggggc agaggcaggt gtgaaattct
      721 ggctgggtgg agtagaaggc ctctttatcg agtcagttta gactggactg aaccgggcca
      781 aaggagccga gcacatcaca cctccccgca ttatttcctg gaacctgggc aatctgaagg
      841 cagagaaagc tgcctggggc ccacagcccg gcactccttc ccgtatcaat tgacgctcaa
      901 atttccgcag taggaatgct caagcattcc ttttggtagc tgccttttgg caggacattc
      961 cggtcagagg gacagagacc cctgcattgc ggcaaaaatg tgcgcatgtg cagccattgc
     1021 ctgggacgca tgcgtaggga gccgcgcgac aaacctgagc cattgcggca agactagcgc
     1081 agagaggaga gggagccgga gatgccagac gcttggttct gaggagtgat ttgcaacgca
     1141 atggagcgag gaaggtcagc tgggcttgtg gattctgcta gcatggtgag caagggcgag
     1201 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1261 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1321 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1381 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1441 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1501 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1561 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1621 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1681 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1741 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1801 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1861 gccgccggga tcactctcgg catggacgag ctgtacaagt aaacgcgtga attcactcct
     1921 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     1981 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     2041 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     2101 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     2161 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     2221 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     2281 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     2341 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     2401 ctcccagtca tagctgtccc tcttctctta tggagatccc ttaattaacg atcatattca
     2461 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
     2521 agtttacgtc cagccaagct taggatctcg acctcgaaat tctaccgggt aggggaggcg
     2581 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     2641 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     2701 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     2761 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     2821 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     2881 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     2941 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     3001 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     3061 ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac cgagtacaag
     3121 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3181 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3241 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3301 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3361 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3421 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3481 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3541 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3601 cacaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3661 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     3721 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatgatatc agatccccgg
     3781 gatgcagaaa ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc
     3841 tgtcatactt tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg
     3901 agctacgggg gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct
     3961 ttactattgc tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc
     4021 aaattaaggg ccagctcatt cctcccactc atgatctata gatctataga tctctcgtgg
     4081 gatcattgtt tttctcttga ttcccacttt gtggttctaa gtactgtggt ttccaaatgt
     4141 gtcagtttca tagcctgaag aacgagatca gcagcctctg ttccacatac acttcattct
     4201 cagtattgtt ttgccaagtt ctaattccat cagaagctgg tcgagatccg gaacccttaa
     4261 tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgaaga ggttcactag
     4321 gcgcgccttc ccgtatcagc tctgaaatct gcagagctga gatctggcag gaagcaggag
     4381 actcagtggg aattctatct gtctaaagtc tttgtgtgag tccagatctc tagatcctta
     4441 cgcaaagagt aaagttagga ggactcctga cttcagggct ggtgggggca gggctggctg
     4501 ccagcctgag agaacaaaac gaagcaactg tgctgtagtt tgagtctggt ctgcaaggtg
     4561 actcctggta taataccagc ccttggaaaa cagagtcctg agagagaaaa aagtaggaga
     4621 aagtttgaag ccagtttgga ccacataaaa aatagactct gtatctaaaa cataactggg
     4681 gccaggtgtg gtgataggca tctttaagcc agtatttgag agtcgaaggc aggaggatgt
     4741 ctgtgagttc aaggcctctc ctgctctata tagcaatttc aggacagcct ctactcggag
     4801 aggctctctc tcaaaaccac aataagccag gtggtggtgg cacaggcctt taatcccagc
     4861 accacttgga aggcagaggc agaggcaggc ggatttatga gtttgaggcc agcctgagtg
     4921 agttccagga cagccagggc tacacagaga aaccctgtct caaaaaaaac aaaaaaacaa
     4981 aaaaaaaaaa aaaaccaata actggttggt gactcctgtc tcttattcta gcacttaaca
     5041 ggctgaggca ggagaattac tgtaaatccc aaggcactct gtgctagaca gtaagacctt
     5101 gtctgaaaga aactgtatat agcaggcata gtggcaaatg ccggccggcc aataagggcg
     5161 aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt
     5221 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     5281 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5341 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5401 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5461 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5521 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5581 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5641 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5701 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5761 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5821 atttaacgcg aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg
     5881 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     5941 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     6001 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     6061 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     6121 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
     6181 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6241 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6301 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6361 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6421 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6481 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6541 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6601 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6661 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     6721 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6781 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6841 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6901 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6961 gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7201 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7441 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8761 gcagcgagtc agtgagcgag gaagcggaag
//
