LOCUS       sequence-26167-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-HA-CUL1 K720A
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(250..270)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        255..584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      435..570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     497..516
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(705..724)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaactcga ggagacgcaa actactgatt caggcggcca tcgtgagaat catggccatg
       61 aggaaggttc tgaaacacca gcagttactt ggcgaggtcc tcactcagct gtcctccagg
      121 ttcaaacctc gagtccctgt gatcaagaaa tgcattgaca ttctaattga gaaagaatat
      181 ttggagcgag tggatggtga aaaggacacc tacagttact tggcttaacc cttctggatc
      241 cagatctgtg gaatgtgtgt cagttagggt gtggacagtc cccaggctcc ccagcaggca
      301 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      541 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      601 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
      661 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      721 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccgnntgtca gcgcangggc
      781 gcccggtntt tttgtcaaga cngacctgtc cggtgccctg aatga
//
