LOCUS       sequence-261670-       11533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-A*01:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A1
FEATURES             Location/Qualifiers
     source          1..11533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1166..1300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1190..1209)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1244..1263
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1442..1464)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2050..3839
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4145..6070)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6505..6523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6591..6695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6696..6764,6765..7556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6914..6933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7727..8315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8216..8235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8932..9077
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9117..10142
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9117..10142
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10163..10182)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10207..10255
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        10908..11287
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11288..11491
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11442..11462
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11488..11512
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcagttc aggaggcgga
      121 tctcattcta tgagatattt cttcacatcc gtgtcccggc ccggccgcgg ggagccccgc
      181 ttcatcgccg tgggctacgt ggacgacacg cagttcgtgc ggttcgacag cgacgccgcg
      241 agccagaaga tggagccgcg ggcgccgtgg atagagcagg aggggccgga gtattgggac
      301 caggagacac ggaatatgaa ggcccactca cagactgacc gagcgaacct ggggaccctg
      361 cgcggctact acaaccagag cgaggacggt tctcacacca tccagataat gtatggctgc
      421 gacgtggggc cggacgggcg cttcctccgc gggtaccggc aggacgccta cgacggcaag
      481 gattacatcg ccctgaacga ggacctgcgt tcttggaccg cggcggacat ggcagctcag
      541 atcaccaagc gcaagtggga ggcggtccat gcggcggagc agcggagagt ctacctggag
      601 ggccggtgcg tggacgggct ccgcagatac ctggagaacg ggaaggagac gctgcagcgc
      661 acggaccccc ccaagacaca tatgacccac caccccatct ctgaccatga ggccaccctg
      721 aggtgctggg ccctgggctt ctaccctgcg gagatcacac tgacctggca gcgggatggg
      781 gaggaccaga cccaggacac ggagctcgtg gagaccaggc ctgcagggga tggaaccttc
      841 cagaagtggg cggctgtggt ggtgccttct ggagaggagc agagatacac ctgccatgtg
      901 cagcatgagg gtctgcccaa gcccctcacc ctgagatggg agctgtcttc ccagcccact
      961 ataccgattg ttggcatcat agccggactt gtattgctgg gtgcggtgat tacgggcgcc
     1021 gtcgtcgctg ctgtgatgtg gcggcggaag tcttcagaca gaaaaggcgg atcttatacc
     1081 caagctgcaa gctcagattc agcacagggg tctgatgtga gtctgacagc ttgcaaagtg
     1141 tgataactcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg
     1201 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1261 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1321 ttttatgttt caggttcagg gggaggtggg gaggtttttt aaagcaagta aaacctctac
     1381 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     1441 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     1501 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     1561 ccggtcgact ctagagcccc gccgccggac gaactaaacc tgactacggc atctctgccc
     1621 cttcttcgct ggtacgagga gcgcttttgt tttgtattcg gggcagtgca tgtaatccct
     1681 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     1741 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     1801 ccaacactga gtggctttca tcctggagca gactttgcat gctgtggact gcaacacaac
     1861 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     1921 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     1981 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     2041 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     2101 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     2161 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     2221 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     2281 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     2341 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     2401 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     2461 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     2521 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     2581 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     2641 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     2701 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     2761 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     2821 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     2881 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     2941 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     3001 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     3061 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     3121 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     3181 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3241 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     3301 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     3361 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     3421 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     3481 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     3541 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     3601 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     3661 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     3721 ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc
     3781 tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc
     3841 atgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
     3901 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
     3961 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
     4021 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
     4081 attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt
     4141 tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc
     4201 cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta
     4261 ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca
     4321 taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga
     4381 ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg
     4441 ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag
     4501 ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga
     4561 cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac
     4621 cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc
     4681 accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt
     4741 ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat
     4801 ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg
     4861 gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg
     4921 gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc
     4981 cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc
     5041 ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg
     5101 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc
     5161 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     5221 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5281 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     5341 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     5401 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
     5461 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     5521 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5581 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     5641 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     5701 cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     5761 ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc
     5821 ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg
     5881 gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc
     5941 cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt
     6001 ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct
     6061 cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt
     6121 gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca
     6181 tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc
     6241 tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc
     6301 tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg
     6361 gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca
     6421 acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac
     6481 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     6961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7201 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     7261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7861 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8341 ggttcctggc cttttgctgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     8401 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     8461 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     8521 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     8581 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     8641 ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     8701 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     8761 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     8821 acgtagccca gcgcgtcggc cgccatgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     8881 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     8941 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     9001 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     9061 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggcaa tgagatatga
     9121 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     9181 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     9241 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     9301 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     9361 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     9421 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     9481 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     9541 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     9601 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     9661 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     9721 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     9781 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     9841 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     9901 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     9961 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    10021 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    10081 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    10141 aggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    10201 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    10261 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    10321 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    10381 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
    10441 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
    10501 cgtggggtct ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
    10561 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
    10621 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
    10681 tgtttgacag cttatcatcg cagatccggg caacgttgtt gcattgctgc aggcgcagaa
    10741 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
    10801 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
    10861 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
    10921 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    10981 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11041 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    11101 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    11161 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    11221 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    11281 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
    11341 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    11401 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    11461 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag
    11521 ctgggtacca gct
//
