LOCUS       sequence-261674-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD268
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             171..476
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(524..547)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(551..574)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(578..601)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    620..653
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1006..1030
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1031..2056)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1031..2056)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2059..2083)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4001..4324
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(6141..6170)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6180..6213
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6231..6251)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      6797..6806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7184..7489)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7761..7777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7761..7777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7774..7796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7785..7801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7809..7815,7816..7833,7834..7839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7854..7875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7992..8009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8163..8751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8243..8262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8922..9713,9714..9782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9545..9564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9783..9887)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9955..9973
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10011..10033)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10133..10152
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10346..10368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10360..10377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10361..10377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga atgaagcccg
       61 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
      121 gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa atgcagttta
      181 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
      241 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
      301 ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
      361 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
      421 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
      481 caggctccct tatacacagc cagtctgcag gtcgaactag tctcttgtca tcgtcatcct
      541 tgtaatcacg cttgtcgtcg tcgtccttgt agtcacgctt atcgtcatcg tctttataat
      601 cagatccggc tccctgaaaa taacttcgta taatgtatgc tatacgaagt tattaaaaat
      661 tgcggtcata aactgaaacg taacatatga taaggtattt tgtgtgcggt tttttctatg
      721 atgtttcaaa aaaatttatc agaagtaaaa aacataagaa gtgtacacag aacattgtgt
      781 agattttggg atagaattat tattaatggg gggagggagc acaatttttt tcgacttttt
      841 attacgaatt aattctattt ttgaatctac acaatttcat tgttagaggt gacttaaaag
      901 aagctaaaaa acaaagaaat taagagaagt tttttgataa taacaaaaat agggggtggg
      961 agcacaggga gaaagagcat gtagggatgt tgaagagtaa ttggacccac tttgtacaag
     1021 aaagctgggt ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     1081 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     1141 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     1201 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     1261 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     1321 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     1381 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     1441 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     1501 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     1561 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     1621 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     1681 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     1741 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     1801 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     1861 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     1921 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     1981 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     2041 gttcaggctt tttcatggag cctgcttttt tgtacaaact tgcggcaccg cctgacatat
     2101 taccttaaaa ttcaaaaatt aatttcagat cagtcataac caagaaaaca aaataaacgt
     2161 taggacagaa aattcaaatt aattaaaaat tatgctgaat aagaaaatta catgcaaagc
     2221 cttattttcc tcatagaaac agttgaataa catatttttt aattgaaaac atagcaaaat
     2281 tcgtggtaaa atttgtcaaa accttggaaa aaatcgaaaa aagaacattt cccgatgcat
     2341 tttcctattt ctcgttgccg acaacgtata aacgttgaat actcagtcaa cgaatttggc
     2401 tgctggcttg gactctcgca cggaagagac gcgtgtgtag atttacgacg aaagcaaaaa
     2461 tgatcaccaa aaacggaacg ttgagctgga cggaaatagt ggtaaagtga catgattata
     2521 gtttgaagat ttctaatttc acaattagag caaatgttgt tcggtattta ttttcaacgg
     2581 tatttatact attttccacc tttttctaga acattcgagc tgcttgttgc aaaaggaggg
     2641 cgactcacat tcggtacatg gaaaagtagt gtacacaata aagagaccca gatacatttt
     2701 ccgtctgcgt ctctttgcac ccaccgggag tattttcaaa cgaatgcatc taggaccttc
     2761 tagaacattc tgtaaggctg cagaatgcgg gtatataagg aaagcgggct cagaggaagc
     2821 caacacgctt tgttctagtg catctaaaaa acttcgaaaa tatgtcaaat cttctcactg
     2881 ttcatcaaaa tcttccagca cttccagttg acgcgacaag tgatgaagtt cgcaagaacc
     2941 tcatggatat gttccgtgac agacaagcct tctccgagca cacctggaag atgcttctct
     3001 cagtgtgccg ttcttgggct gcctggtgca agcttaacaa ccgcaagtgg ttcccagccg
     3061 agccagaaga cgttcgtgac tacttgctct acctccaagc tcgtggattg gcggtgaaga
     3121 cgatccaaca acatctcgga cagctcaata tgctccatcg ccgatctgga cttccacgcc
     3181 catcagattc taatgccgta tctctcgtaa tgagacgtat ccgcaaggaa aatgttgatg
     3241 ctggagagag agccaagcaa gccctcgcct tcgaaaggta agtttaaata ttaatgtatt
     3301 caatcctaag acttgacgaa attttcagaa ccgacttcga tcaggtccgt tctcttatgg
     3361 aaaattccga tcgttgccag gacatccgaa acctcgcctt cctcggaatt gcttacaaca
     3421 cacttctccg tatcgccgaa attgcccgca ttcgcgtcaa ggatattagt cgcacagacg
     3481 gaggaagaat gctcatccac atcggacgta caaaaacgct ggtctcgact gccggagtcg
     3541 aaaaagcgct ctcccttgga gttaccaagc tcgttgagcg ctggatttcg gtttccggag
     3601 tcgccgatga tccaaacaac tacctgttct gtcgtgttcg caagaacgga gttgctgccc
     3661 cgagtgcaac atcacaactc tcgactcgag ctcttgaagg taagtttaaa caacatatta
     3721 gctaagaata agctgaaaat aaattttcag gaatcttcga agctacccac cgtctcatct
     3781 acggagccaa ggatgattcc ggacaacgtt acttggcttg gtctggacat tccgcccgtg
     3841 tcggagctgc ccgtgacatg gctcgcgctg gagtttccat cccagagatt atgcaagccg
     3901 gaggttggac caacgtcaac attgtgatga actatattag aaacttggat tctgaaactg
     3961 gcgccatggt tcgtcttctc gaagacggtg attaagataa atgcaaaatc ctttcaagca
     4021 ttcccttctt ctctatcact cttctttctt tttgtcaaaa aattctctcg ctaatttatt
     4081 tgctttttta atgttattat tttatgactt tttatagtca ctgaaaagtt tgcatctgag
     4141 tgaagtgaat gctatcaaaa tgtgattctg tctgatgtac tttcacaatc tctcttcaat
     4201 tccattttga agtgctttaa acccgaaagg ttgagaaaaa tgcgagcgct caaatatttg
     4261 tattgtgttc gttgagtgac ccaacaaaaa gaggaaactt tattgtgccg ccaagaaaaa
     4321 agtctcactg aatctcaaat attttattag aaaacaccaa caatttagat atttctgtat
     4381 ccacggttgg cgttgacagc ctctgatggt ggagacttgt ctgggcactg gcagtactcg
     4441 gcatcctttc ctggtcttcc gtctggtcca gtctctccct gggttccgtc tggtccttga
     4501 cgtccttgct ttccagatgg tccttctggt ccaacggttc ctggctctcc tggtggtccg
     4561 tgaagaccgt tctgtccagc tggtcctgtt ggtccttgat ctcctcttgg tccctttggt
     4621 cctggtcttc cgattggatg ctcggcgtcg cgtcccttct ctcctggtgg tcctggctct
     4681 ccgatctttc cgatttgacc ggatgatcct tgctctcctg gatgtcctgg ttgtccatct
     4741 cttcctgggt ttccattttg tcctcttggt cctggaagtc cacgtggtcc tggtcttcca
     4801 agagctcctg gtggtccaac tggtccttgt gggcaagtga agcatccgac agactcgcgt
     4861 tgtggagcga tatcatcagc tccaactcct ggaacaccgt caagtcctgg aactccgtcg
     4921 agtcctggaa ctcctggaac tccctttggt ccggatggtc cagctgggca cttgttatca
     4981 gcattgcagt tgcaggttgg tccatttcca ttggctccat ttggagtctt tggctggttg
     5041 aagactggtg gaactgctgg tggttgacct ggtggagcag atggggatcc atcagcatgt
     5101 ggagttgggg tagcgttggt ctcttcatat tgacaacgga cacgcttgct agatcttccg
     5161 atgacaacta tgtccttcca caatccattg gtagattgct agaaaattaa aaattattta
     5221 aaattcaaaa tataatttaa ctcactctga acacattcat ctcggtatca agcttctctc
     5281 tgaatccatc aacctccgag tagatggttg acatggtcat gaagcatacc ataagagtgg
     5341 cgacagtgac cgaagccgtc acataacacg caagttttac agacatctct gaaattatca
     5401 aatttaggat ttcaaaataa ttcggccaaa gaaatgtaac tcacctagtc ttcgagaagc
     5461 agagacttgt cgatttccta ccggattcgt ctccttttat taggaaatct ttccgcaagt
     5521 tttcaagttt tttttgtttt ttttttacat taccgacgag acagtgtgcg agacgcagag
     5581 acgataaaag actggataag aacgggagat aaggagtacg gatgtaggtg aggaatgtgg
     5641 ccgaaaggac agatgggctt tattcgggaa gattggttgg gaaatgaacc agagaagaat
     5701 gactgaataa agttgttgaa acttggtgag aatggaagat agacaatcga gggcagctag
     5761 tttccttcct cctctatata tttttatgtt ttcttttaaa ccttcctcca tatttctgaa
     5821 ttgaatgttg ttgcatattg tgtgtcctta tttcatttcc atcatccttt tgtgtgtttc
     5881 ccccttccac caattccccc cacgtcctaa tgaaatgaaa gcgagaagat aaataaactt
     5941 gcacattgtg ttcagatgag aggagcggaa tacgaattgg gagacggaaa gagaaaaaaa
     6001 aatgtttaga tttggattga attaattttt tagaaagcaa gaaaaaaaac acaaagagaa
     6061 taaataaata ataggaaaaa aaacaaatat tcagtattta aaatttgaaa attcaacgac
     6121 gttggcgtcg atcatcctta gaggtcctcc tcggagataa gcttttgctc tccctgaaaa
     6181 taacttcgta taatgtatgc tatacgaagt tattttaaac ttacctttcc ggattgaaag
     6241 tacagattct ccttgtagag ctcgtccatt cccatgacgt cggtgaaggc cttttgccac
     6301 tccttgaagt tgagctcggt cttggagtgc ttgagctcgg tcttacggaa gacgtacatt
     6361 ggttggttct tgaggtagtt ggcggccatt ggcttggcga aggtgtaggt ggtacgggcg
     6421 gtggaacggt aacgctttcc gtttccggtg gtgtaggacc acttgaaggt ggagatgatg
     6481 gtctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact taccttgtcg
     6541 tttgggtagg tcttcttgga acggcaccag tcggcggcgg tgagggagtt ggtcatgact
     6601 ggtccgtcgg ctgggaatcc ggttcccttg acttgggcct ctccctgaaa atttaaatat
     6661 gtatggttag ttagtaccga actgtttaaa cttaccttga tgtgggatcc ctcgtaggtg
     6721 taacggtagt tgacggtgag ggaggctccg tcctcgaatt gcatggtacg gtggacttgg
     6781 tatccggatc cgtcgaccat ggcggcttgg aatggggaca ttccgtctgg gtatgggagg
     6841 tattggtgga atccgtatcc gatgtgtggg acgaggatcc atggggagaa ttggaggtct
     6901 ccctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact taccttggtg
     6961 gacttgaggt tgagctcctc gtatccgtcg tttgggtttc cggttccttg tccgaccatg
     7021 tcgaagtcga ctccgttgat ggatccgaag atgtggagct cgtgggtggc tgggagggag
     7081 gccatgttgt cctcctctcc cttggagacc atcgatgctc ctgaggctcc cgatgctccc
     7141 taggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     7201 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     7261 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     7321 cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg
     7381 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     7441 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     7501 ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     7561 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     7621 gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     7681 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     7741 tcctaggaat cgataacatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7801 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7861 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7921 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8041 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8101 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8581 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8761 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8821 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9061 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9901 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9961 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10201 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcgc
    10381 cggcactagt tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
    10441 gacagttgac agcgacagct atcagttgct ca
//
