LOCUS       sequence-26168-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-HA-CUL1 Y42A/M43A
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             134..160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacctccc
      181 ggactatgca gggcccatgg cgtcaacccg gagccagaac ccccacggcc tgaagcagat
      241 tggcctggac cagatctggg acgacctcag agccggcatc cagcaggtgt acacacggca
      301 gagcatggcc aagtccagag ctgcagagct ctacactcat gtttataact actgtactag
      361 tgttcaccag tcaaaccaag cacgaggagc tggagttcct ccttctaagt cgaaaaaggg
      421 gcagacacct ggaggagctc agtttgttgg cctggaatta tataaacgac ttaaggaatt
      481 tttgaagaat tacttgacaa atcttcttaa ggatggagaa gatttgatgg atgagagtgt
      541 actgaaattc tacactcaac aatgggaaga ttatcgattt tcaagcaaag tgctgaatgg
      601 aatttgtgcc tacctcaata gacattgggt tcgccgtgaa tgtgacgaag gacgaaaagg
      661 aatatatgaa atctattcgc ttgcattggt gacttggaga gactgtctgt tcaggccact
      721 gaataaacag gtaacaaatg ctgttttaaa gctgattgaa aaggaaagga atggtgaaac
      781 catcaataca agattgatta gtg
//
