LOCUS       sequence-26169-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-HA-CUL1 delta 610-615
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             133..159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattca tgtacccata cgatgttcct gactatgcgg gctatcccta tgacctcccg
      181 gactatgcag ggcccatggc gtcaacccgg agccagaacc cccacggcct gaagcagatt
      241 ggcctggacc agatctggga cgacctcaga gccggcatcc agcaggtgta cacacggcag
      301 agcatggcca agtccagata tatggagctc tacactcatg tttataacta ctgtactagt
      361 gttcaccagt caaaccaagc acgaggagct ggagttcctc cttctaagtc gaaaaagggg
      421 cagacacctg gaggagctca gtttgttggc ctggaattat ataaacgact taaggaattt
      481 ttgaagaatt acttgacaaa tcttcttaag gatggagaag atttgatgga tgagagtgta
      541 ctgaaattct acactcaaca atgggaagat tatcgatttt caagcaaagt gctgaatgga
      601 atttgtgcct acctcaannn nnnnngggtt cgccgtgaat gtgacgaang acgaaaangn
      661 nnatatgaaa tctattcgct tgcattggtg acttggagag actgtctgnt cnnnccactg
      721 aataaacagg taacaaatgc tgttttaaag ctgantgaaa aggaaaggaa tggtgaaa
//
