LOCUS       sequence-261699-       10591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der 
            Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, 
            Lippincott-Schwartz J
  TITLE     A contractile and counterbalancing adhesion system controls the 3D 
            shape of crawling cells.
  JOURNAL   J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. 
            Epub 2014 Apr 7.
  PUBMED    24711500
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201311104"; journalName: "J Cell Biol"; date: 
            "2014-04-14- 14"; volume: "205"; issue: "1"; pages: "83-96"
COMMENT     Sequence Label: mApple-MyosinIIA-N-18
FEATURES             Location/Qualifiers
     source          1..10591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        191..494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        495..698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     649..669
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6671..6680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6677..7387
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     7090..7109
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7509..7630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7546..7565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7600..7619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7637..8092)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7774..7795)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7986..8005
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8119..8223
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8225..8582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8225..8416
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8248..8268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8433..8568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8617..9411
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8617..9411
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8671..8690)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9281..9300
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9599..9618)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9643..9690
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(10019..10591,1..16)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10508..10527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
       61 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      121 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      181 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      481 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      541 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      601 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      661 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      721 gctagcgcta ccggactcag atctcgaggc caccatggct cagcaggctg cagacaagta
      781 cctctatgtg gataaaaact tcatcaataa cccgctggcc caagctgact gggctgccaa
      841 gaagttggta tgggtgcctt ccagcaagaa tggctttgaa ccagccagcc tcaaggagga
      901 ggtgggagaa gaggccattg tagagctggt agagaatggg aagaaggtga aggtgaacaa
      961 ggacgacatc cagaagatga acccacccaa gttctccaag gtggaggaca tggcagagct
     1021 cacgtgcctc aacgaagctt cggtgctgca caacctcaag gagcgatact actcagggct
     1081 tatctacacc tattcaggcc tgttctgtgt ggtcatcaac ccttataaga acctgcccat
     1141 ctactcagag gagatcgtgg agatgtacaa gggcaagaag aggcacgaga tgccacccca
     1201 catctacgcc atcacagata ctgcctaccg gagcatgatg caggaccggg aagatcagtc
     1261 catcctgtgc acgggggagt ctggagcagg gaagacagag aacaccaaga aagtcatcca
     1321 gtacctggca catgtggcct cctcacacaa gagcaagaag gaccaggggg agttggagcg
     1381 gcagctgcta caggccaacc ctatcctaga ggcctttgga aacgccaaga cggtgaagaa
     1441 tgacaactcc tctcgattcg gtaaattcat tcgtatcaac tttgatgtca atggctacat
     1501 tgttggtgcc aacattgaga cttatcttct ggagaaatct cgtgctatcc gccaagccaa
     1561 agaggagcgg accttccaca tcttctacta cctgctgtct ggggccggag aacacctgaa
     1621 gactgatctc ctgttggagc catacaacaa ataccgcttc ctgtccaacg ggcacgtcac
     1681 catccctggg cagcaggaca aggacatgtt ccaggagaca atggaggcca tgagaattat
     1741 gggtatccca gaggatgagc agatgggctt gctgcgggtc atctctgggg tccttcagct
     1801 tggcaacatt gccttcaaga aggagcggaa cactgaccag gcgtccatgc cggacaacac
     1861 agctgctcaa aaggtgtccc acctcctggg gatcaatgtg accgacttca ccagaggcat
     1921 cctcacccca cgcatcaagg tgggcagaga ctatgtgcag aaggcgcaga ctaaagagca
     1981 ggctgacttt gccattgagg ccttggccaa ggctacctat gagcggatgt tccgctggct
     2041 ggtgcttcgc atcaacaaag ctctggacaa gaccaagagg cagggcgcct catttatcgg
     2101 gatactggac atcgctggct ttgaaatctt tgatctgaac tccttcgagc agctgtgcat
     2161 caactacacc aacgagaagc tgcagcagct gttcaaccac accatgttca tcctggagca
     2221 ggaggagtac cagcgagagg gcatcgagtg gaacttcatc gacttcggcc tggacctgca
     2281 gccctgcatc gacctcattg agaagccggc gggtccccca ggcatcctgg ccctgctaga
     2341 tgaggagtgc tggtttccta aggccactga caagagcttc gtggagaagg tggtgcagga
     2401 gcagggcacc caccccaagt tccagaagcc caagcaactg aaggacaagg ctgatttctg
     2461 cattatccac tatgccggca aggtggacta taaagctgac gagtggctga tgaagaacat
     2521 ggaccccttg aacgacaaca tcgccacgct gcttcaccag tcctcagaca agtttgtctc
     2581 tgagctgtgg aaggatgtgg atcggatcat tggcttggac caagtggctg gaatgtccga
     2641 gacagcacta cctggtgcct tcaagacccg gaagggcatg ttccgtactg tcggacagct
     2701 gtacaaggag cagctggcca agctcatggc cacgttgagg aataccaacc ccaacttcgt
     2761 gcgctgcatc attcccaacc atgagaagaa ggccggcaaa ctggacccgc acttggtgct
     2821 ggaccagctg cgctgcaatg gcgtccttga gggcatccgg atctgccgcc agggctttcc
     2881 caacagggtg gtcttccagg agttccggca gaggtatgag atcctcaccc ccaactccat
     2941 cccgaagggc ttcatggatg gcaagcaagc gtgtgtgctc atgatcaaag ccttggagct
     3001 tgacagcaac ctgtaccgca tcggccagag caaagtgttc ttccgggcag gagtgctcgc
     3061 ccacctggag gaagagcggg acctgaagat caccgatgtc atcattggct tccaggcctg
     3121 ctgcaggggc tacctggcca ggaaggcctt tgccaagagg cagcaacagc tgaccgccat
     3181 gaaggtccta cagaggaact gtgctgcgta cctcaggctg cgcaactggc agtggtggag
     3241 gctcttcacc aaggtcaagc ccctgttgaa ctcaataaga catgaggatg agctgttagc
     3301 caaggaggcg gaactgacaa aggttcgaga gaaacatctg gctgcagaga acaggctgac
     3361 agagatggag acgatgcagt ctcagctcat ggcagagaag ctgcagcttc aggagcagct
     3421 gcaggcggag acagagctgt gtgccgaggc tgaggagctc cgggcccgtc tgacagcgaa
     3481 gaagcaggag ctggaggaga tatgccatga cctggaggcc agggtggagg aggaggagga
     3541 gcgctgccag tacctgcagg ccgagaagaa gaagatgcag cagaacatcc aggaacttga
     3601 ggagcagttg gaggaggagg agagcgcccg gcagaagctg cagcttgaga aggtgaccac
     3661 cgaggccaag ctgaagaaac tggaggagga ccagatcatc atggaggacc agaactgcaa
     3721 actggccaag gagaagaaac tgctggaaga cagagtagct gagttcacta ccaacctcat
     3781 ggaagaggag gagaagtcca agagcctggc caagctcaag aacaagcacg aggcaatgat
     3841 caccgacctg gaagagcgcc tccgtaggga ggagaagcag aggcaggagt tggagaagac
     3901 ccgtcgcaag ctggagggag actccacaga cctcagtgac cagattgctg agctccaggc
     3961 gcagatagca gagctcaaga tgcagctggc caagaaggag gaggagttgc aggctgcctt
     4021 ggccagagtg gaagaagaag ctgctcagaa gaatatggcc ctgaagaaga tccgagaact
     4081 ggaaactcag atatctgagc tccaggagga cctggagtcg gagcgagcct ccaggaataa
     4141 agccgagaag cagaaacggg atctgggaga ggagctggag gcgctgaaga cagagctgga
     4201 ggacacgctg gactccacgg ctgcccagca ggagctgagg tcgaagcgtg agcaggaggt
     4261 gagcatcctg aagaagactc tggaggacga ggccaagacc catgaggccc agatccagga
     4321 gatgaggcag aagcactcac aggctgtgga ggagctggca gatcagttgg agcagacgaa
     4381 gcgggtaaaa gctacccttg agaaggcgaa gcagaccctg gagaatgagc ggggagagct
     4441 ggccaatgag gtgaaggccc tgctgcaagg caagggcgac tcagagcaca agcgcaagaa
     4501 ggtggaggcg cagctgcaag aactgcaggt caagttcagc gagggagagc gcgtgcgaac
     4561 cgaactggcc gacaaggtca ccaagctgca ggttgaactg gacagtgtga ccggtctcct
     4621 tagccagtct gacagcaagt ccagcaagct tacgaaggac ttctctgcgc tggagtccca
     4681 gcttcaggac acacaggagt tgctccagga ggagaaccgg cagaagctga gcctgagcac
     4741 caagctcaag cagatggagg atgagaaaaa ctccttcagg gagcagctgg aggaggagga
     4801 ggaggccaag cgcaacttgg agaagcagat cgccacgctc catgcccagg tgaccgacat
     4861 gaagaagaag atggaggacg gtgtagggtg cctggagact gcagaggagg cgaagcggag
     4921 gcttcagaag gacttggaag gcctgagcca gcggcttgag gagaaggtgg ctgcctacga
     4981 taagctggag aagaccaaga cacggctgca gcaggagctg gacgacctgc tggttgacct
     5041 ggaccaccag cggcagagcg tctccaacct ggaaaagaag cagaagaagt tcgaccagct
     5101 cctagccgag gagaagacca tctcggccaa gtatgcagag gagcgtgacc gagctgaggc
     5161 tgaggcccgt gagaaggaga caaaggcgct atcactggcc cgggcgcttg aggaggccat
     5221 ggagcagaag gcagagctgg agcggctcaa caagcagttc cgcacggaga tggaggacct
     5281 catgagctcc aaggatgacg tgggcaagag tgtccacgag ctggagaagt ccaagcgggc
     5341 cttggagcag caggtggagg agatgaagac ccagctggag gagctggagg atgagctgca
     5401 ggccacggag gatgccaagc tccgcctgga ggtgaacctg caggccatga aggcccagtt
     5461 tgagcgggat ctgcagggcc gggatgaaca gagcgaggag aagaagaagc agctggtcag
     5521 acaggtgcgg gagatggagg cggagctgga ggatgagagg aagcagcgct ccatggccat
     5581 ggccgcacgc aagaaactgg agatggatct gaaggacctg gaggcacaca ttgacacagc
     5641 caataagaac cgggaagagg ccatcaaaca gctgcggaag cttcaggccc agatgaagga
     5701 ctgcatgcgg gagctggacg acacgcgcgc ctcccgggag gagatcctgg cgcaggccaa
     5761 ggagaatgag aagaagctga agagcatgga ggccgagatg attcagctgc aggaggaact
     5821 ggcagctgct gagcgtgcta agcgtcaggc ccaacaggaa cgggacgagc tggctgatga
     5881 gatcgccaac agcagtggca aaggggccct agcattagag gagaagcggc gactggaggc
     5941 ccgcattgcc ctgctggagg aggagctgga ggaggaacag ggcaacacgg agctgatcaa
     6001 cgatcggctg aagaaggcca acctgcagat cgaccaaata aacaccgacc tgaacctgga
     6061 acgcagccac gcacagaaga atgagaatgc gcgacagcag ctggaacgcc agaacaagga
     6121 gctcaaggcc aagctgcagg aaatggagag tgctgtcaag tccaaataca aggcctccat
     6181 cgcggccttg gaggccaaaa ttgcacagct ggaggaacag ctggacaacg agaccaagga
     6241 gcgccaggca gcctccaagc aggtgcgccg gacggagaag aagctgaagg acgtgctgct
     6301 gcaggtggag gacgagcgga ggaacgcgga acagttcaag gaccaggctg acaaggcgtc
     6361 cacccgcctg aagcagctta aacggcagct agaggaggct gaagaggagg cccagcgggc
     6421 caatgcctca cgccggaagc tgcagcgtga gctggaagat gccacagaga ccgctgatgc
     6481 tatgaaccgc gaggtcagct ccctgaagaa caaactgagg cgtggggacc tgccatttgt
     6541 cgtgactcgc cgaattgttc ggaaaggcac tggcgactgc tcagacgagg aggtcgacgg
     6601 taaagcagat ggggccgatg ccaaggcagc tgaaggcgga agcggcggga gcgcagggga
     6661 tccaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga
     6721 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     6781 gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa
     6841 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     6901 ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
     6961 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga
     7021 ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc
     7081 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg aggagcggat
     7141 gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc tgaaggacgg
     7201 cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     7261 cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat
     7321 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     7381 caagtaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     7441 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7501 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7561 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7621 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7681 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7741 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7801 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7861 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7921 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7981 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8041 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     8101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8221 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8521 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8581 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     8641 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     8701 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8761 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     8821 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     8881 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     8941 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9001 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9061 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9121 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     9181 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9241 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9301 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9361 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     9421 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     9481 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9541 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     9601 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     9661 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     9721 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     9781 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     9841 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     9901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10561 agcgtcgatt tttgtgatgc tcgtcagggg g
//
