LOCUS       sequence-261712-       11417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 gap repair vector -  KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11417)
  TITLE     Ellis Lab Yeast CRISPR Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWS173
FEATURES             Location/Qualifiers
     source          1..11417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4879..4899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4903..4923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             5833..5844
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5851..6564
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6576..6647
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6663..6690
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        6704..6779
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gene            7290..8645
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             7634..8443
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      8448..8645
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      8650..9632
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(9690..10505)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9690..10505)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10497..10516
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10730..11318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11219..11238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagactg aactggccga taattgcaga cgaacggtga
       61 gtaaggaaag agtgaggaac tatcgcatac ctgcatttaa agatgccgat ttgggcgcga
      121 atcctttatt ttggcttcac cctcatacta ttatcagggc cagaaaaagg aagtgtttcc
      181 ctccttcttg aattgatgtt accctcataa agcacgtggc ctcttatcga gaaagaaatt
      241 accgtcgctc gtgatttgtt tgcaaaaaga acaaaactga aaaaacccag acacgctcga
      301 cttcctgtca tcctattgat tgcagcttcc aatttcgtca cacaacaagg tcctagcgac
      361 ggctcacagg ttttgtaaca agcaatcgaa ggttctggaa tggcgggaaa gggtttagta
      421 ccacatgcta tgatgcccac tgtgatctcc agagcaaagt tcgttcgatc gtactgttac
      481 tctctctctt tcaaacagaa ttgtccgaat cgtgtgacaa caacagcctg ttctcacaca
      541 ctcttttctt ctaaccaagg gggtggttta gtttagtaga acctcgtgaa acttacattt
      601 acatatatat aaacttgcat aaattggtca atgcaagaaa tacatatttg gtcttttcta
      661 attcgtagtt tttcaagttc ttagatgctt tctttttctc ttttttacag atcatcaagg
      721 aagtaattat ctacttttta caacaaatat aaaacaagat ctatggacaa gaagtattct
      781 atcggactgg atatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag
      841 gtgccctcta agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat
      901 ctgatcggag ctctcctctt tgattcaggg gaaaccgctg aagcaacccg cctcaagcgg
      961 actgctagac ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc
     1021 tccaacgaaa tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc
     1081 gtggaagagg ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg
     1141 gcctatcacg agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc
     1201 gacaaggcag acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc
     1261 cacttcctga tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc
     1321 cagctggtgc agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc
     1381 gatgccaagg ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc
     1441 gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt
     1501 gggctgactc ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg
     1561 tccaaggaca cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac
     1621 gccgaccttt tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc
     1681 cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat
     1741 gagcaccacc aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag
     1801 tacaaggaaa tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt
     1861 gcctcccaag aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc
     1921 gaagagctcc tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac
     1981 aatgggagca ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa
     2041 gaggacttct acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc
     2101 cgcatcccct actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc
     2161 agaaagtcag aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc
     2221 agcgctcagt cattcatcga acggatgact aacttcgata agaacctccc caatgagaag
     2281 gtcctgccga aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag
     2341 gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag
     2401 gcaattgtgg accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag
     2461 gactatttca agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg
     2521 ttcaacgcaa gcctgggtac ttaccatgat ctgcttaaga tcatcaagga caaggatttt
     2581 ctggacaatg aggagaacga ggacatcctt gaggacattg tcctgactct cactctgttc
     2641 gaggaccggg aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa
     2701 gtgatgaagc aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc
     2761 atcaacggaa tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac
     2821 ggattcgcta atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag
     2881 gacatccaga aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat
     2941 ctggctggtt cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag
     3001 ctggtcaagg tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa
     3061 aaccagacta cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa
     3121 gggattaagg agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt
     3181 cagaatgaga agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa
     3241 gagcttgata tcaacaggct ctcagactac gacgtggacc atatcgtccc tcagagcttc
     3301 ctcaaagacg actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag
     3361 tcagataacg tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt
     3421 ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc
     3481 ggactgagcg aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag
     3541 attactaaac atgtcgccca aatccttgac tcacgcatga ataccaagta cgacgaaaac
     3601 gacaaactta tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc
     3661 agaaaggact ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac
     3721 gcatatctga atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc
     3781 gagttcgtgt acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag
     3841 caggagatcg gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc
     3901 aagaccgaaa tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat
     3961 ggggagactg gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg
     4021 ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct
     4081 aaggaatcaa tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg
     4141 gaccctaaga agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg
     4201 gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc
     4261 accattatgg agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt
     4321 tacaaggaag tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg
     4381 gagaatgggc ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg
     4441 gctctcccct ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag
     4501 gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg
     4561 gacgagatca ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac
     4621 ctcgataaag tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct
     4681 gagaatatca tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac
     4741 ttcgacacca ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc
     4801 ctcatccacc agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga
     4861 ggggatgagg gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     4921 gtataaggat cctaactcga gattgaattg aattgaaatc gatagatcaa tttttttctt
     4981 ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg aatatttttt
     5041 atttatatac gtatatatag actattattt atcttttaat gattattaag atttttatta
     5101 aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca ttcgatattt
     5161 ctatgtgctg ccaagatatc aacaacagag gacatatgcc ctacctccat gatctactga
     5221 cgtccctgag gctgcaattc atgtaatggg gcagtatccg cggcaagtcc tagtgcaatg
     5281 gcggtttttt accctcgttc tgaagaagag gcgacgcggg tgcggtcatc actaatgtgg
     5341 aaattggtaa gactctcggg cctccgcctt taggcggtgc ttactctttc ataaaggggc
     5401 tgttagttat gcaacgatgt gcttcagtat tacatttttt gccttcaacg ccttgattgt
     5461 tctatttttg ctaataataa atctatttca tcggactaaa agtccattag ttgtaagcgg
     5521 atttagctca gttgggagag cgccagactg aagaaaaact tcggtcaagt catctggagg
     5581 tcctgtgttc gatccacaga attcgcagat ggccggcatg gtcccagcct cctcgctggc
     5641 gccggctggg caacaccttc gggtggcgaa tgggacttga gacggaaagt gaaacgtgat
     5701 ttcatgcgtc attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca
     5761 tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct
     5821 tagctactag agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt
     5881 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt
     5941 ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact
     6001 ggtaaactgc cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc
     6061 tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa
     6121 ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg
     6181 gaagtgaaat ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt
     6241 aaagaggacg gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt
     6301 tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac
     6361 gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat
     6421 ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat
     6481 ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg
     6541 catggtatgg atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga
     6601 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     6661 ctggctcacc ttcgggtggg cctttctgcg tttatacgtc tcagttttag agctagaaat
     6721 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     6781 ttttttattt tttgtcacta ttgttatgta aaatgccacc tctgacagta tggaacgcaa
     6841 acttctgtct agtggatagc tggacattcc aggcagtgcc tctgccgccg gacccctctc
     6901 gtgattgggt agctggacat gcccttgtaa gatataacaa gagcctgcct gtctaatgat
     6961 ctcacggcga aagtcgggga gacagcagcg gctgcagaca ttataccgca acaacactaa
     7021 ggtgagataa ctccgtacat tgactacgcg ttcctctaga ccttacttga ccggatacag
     7081 tgtctttgac acgtttatgg gttacagcaa tcacatccaa gactggctat gcacgaagca
     7141 actcttgagt gttaaaatgt tgacccctgt atttgggatg cgggtagtag atgagtgcag
     7201 gatatcagca gttacagaga tgttacgaac cactagtgca ctgcagtaca gtttagcttg
     7261 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
     7321 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
     7381 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     7441 attacggctc ctcgctccag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     7501 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     7561 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     7621 aacataaaca aaaatgggta aggaaaagac tcacgtttcg aggccgcgat taaattccaa
     7681 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     7741 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     7801 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     7861 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     7921 cactgcgatc cccggcaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     7981 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     8041 ttgtcctttt aacagcgatc gcgtatttcg tctggctcag gcgcaatcac gaatgaataa
     8101 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     8161 ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     8221 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     8281 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     8341 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     8401 gaataaattg cagtttcatt tgatgctcga tgagtttttc taaagtaact gacaataaaa
     8461 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     8521 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     8581 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     8641 tactggagta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     8701 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     8761 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     8821 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     8881 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     8941 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     9001 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     9061 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     9121 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     9181 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     9241 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     9301 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     9361 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     9421 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     9481 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     9541 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc atcagagcgc ttttggtttt
     9601 caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc ccgagcggcc
     9661 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     9721 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     9781 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     9841 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     9901 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     9961 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
    10021 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
    10081 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
    10141 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
    10201 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
    10261 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
    10321 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
    10381 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
    10441 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
    10501 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
    10561 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
    10621 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
    10681 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10741 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10801 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10861 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10921 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10981 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11041 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11101 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11161 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11221 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11281 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11341 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11401 attctgtgga taaccgt
//
