LOCUS       sequence-261713-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human VprBP wildtype in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, 
            Fan HY, Chen X, Xiong Y
  TITLE     CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin 
            Binding of TET Dioxygenases.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):247-60. doi: 
            10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31.
  PUBMED    25557551
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.12.002"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "247-60"
COMMENT     Sequence Label: pcDNA3-HA-VprBP
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             685..711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    724..748
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2016..2027)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5346..5370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5404..5422)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5405..5422)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5442..5459)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5448..5672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5718..6146
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5805..5824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6015..6036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6155..6175)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6160..6489
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6340..6475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6402..6421
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6556..7350
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6610..6629)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7220..7239
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7524..7645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7561..7580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7615..7634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7694..7710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7694..7710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7707..7729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7718..7734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7742..7748,7749..7766,7767..7772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7787..7808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7925..7942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8096..8684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8176..8195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8855..9646,9647..9715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9478..9497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9716..9820)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9895..9914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac catgtaccca tacgacgtcc cagactacgc tgaattcgat
      721 atcacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc
      781 ggtaccgaat tcacaggcaa agccatgact acagtagtgg tacatgtgga ctccaaagct
      841 gagctcacta ccctgctgga gcagtgggaa aaggaacatg gcagtgggca ggacatggta
      901 cctatcctta ccaggatgtc tcaattgatt gaaaaagaaa ctgaagagta tcgtaaaggg
      961 gatccagacc catttgatga tcgacatcct ggtcgagctg atccagagtg tatgctgggc
     1021 cacttgctga gaatactctt caagaatgat gatttcatga atgcactggt gaatgcatat
     1081 gtgatgacaa gccgagagcc ccctttaaac actgcagctt gcagactcct attagacatc
     1141 atgccagggc tggaaactgc tgtcgtcttt caagaaaagg agggaattgt cgagaatctt
     1201 ttcaaatggg cccgagaggc cgatcaacca ttgaggacat attctactgg actgttagga
     1261 ggtgctatgg aaaatcaaga cattgctgcc aactatagag atgaaaattc acagctggtg
     1321 gcaatagtgc ttcgaagact gagggagcta cagctacagg aagtggcttt gcggcaggaa
     1381 aacaagcgtc ccagtccacg gaagctctct tctgaacccc ttttgcctct ggatgaggag
     1441 gctgtggata tggactatgg tgacatggct gtagatgtag tggatggaga ccaagaggaa
     1501 gcttctggag acatggagat ctcctttcat cttgattcag gccacaagac tagtagcaga
     1561 gtgaactcaa caaccaaacc tgaggatgga ggattaaaga aaaacaagtc agcaaaacag
     1621 ggtgacagag agaactttag gaaagccaag caaaagttgg gtttctcatc ttctgatcca
     1681 gatcgcatgt ttgttgagct gtctaatagc agttggtcag aaatgtctcc ctgggtgatt
     1741 ggcaccaatt atacccttta tcctatgact cctgctatcg agcagcgact cattctccaa
     1801 tatttgaccc ctctaggaga atatcaggag ctacttccca tattcatgca acttggatca
     1861 cgggagctga tgatgttcta tattgacctg aagcaaacta atgatgtcct gcttacattt
     1921 gaggcactaa agcacctagc atctctcctg ctccataaca aatttgccac agaatttgtt
     1981 gcgcatggtg gagtacagaa attactggaa atacctcgtc cttctatggc tgcaaccggt
     2041 gtgtctatgt gtttgtatta cctatcctac aatcaggatg ccatggaaag agtttgcatg
     2101 catccccaca atgttctgtc tgatgtggtg aactataccc tgtggttaat ggagtgttct
     2161 catgcttcag gatgctgcca tgctaccatg tttttttcaa tttgcttctc atttcgggcc
     2221 gtcttggagc tctttgaccg ctatgatggt cttcgtcgtc tggtgaactt gatcagtact
     2281 ttggagattc taaatttgga agatcagggt gcacttctga gtgatgatga aatatttgct
     2341 agccgccaaa ctgggaaaca tacctgcatg gccttgcgca aatactttga ggctcacctg
     2401 gccattaaat tggaacaagt gaagcagtca cttcagagga ctgagggtgg cattcttgtc
     2461 cacccacaac ccccgtacaa ggcatgctca tatactcatg aacagattgt ggaaatgatg
     2521 gaatttttga tagaatatgg cccagcgcag ctatattggg aaccagctga agttttcctc
     2581 aaactttctt gtgtgcaact cttgttgcag cttatttcta ttgcctgcaa ttggaagacc
     2641 tattatgcaa ggaatgacac tgtgcgcttt gctttggatg tcctggctat tcttactgtg
     2701 gtgccaaaaa tccagctcca gttggcagaa tcagtggacg tgttggatga ggctggatct
     2761 acagtctcta ctgtaggtat cagcattatt ttgggagtgg ctgagggtga gttcttcatc
     2821 catgatgctg aaattcagaa gtcagcactt cagattatca tcaattgtgt gtgtggccca
     2881 gataaccgaa tatccagtat tggtaaattt atctctggta ctcctcggag aaagctgcct
     2941 cagaacccta aaagcagtga gcacaccctg gccaagatgt ggaatgtggt tcagtccaac
     3001 aacggcatca aggtgctcct gtccttactg tccattaaga tgcccatcac agatgcagac
     3061 caaatccggg ccctggcctg caaagcccta gtgggcctgt ctcgcagtag cactgtccgg
     3121 cagatcatca gtaaactgcc ccttttcagc agctgccaga tccagcagct gatgaaggag
     3181 cctgtgctgc aggacaagcg cagtgaccat gtcaagttct gcaagtatgc tgctgaactc
     3241 attgaacggg tgtcaggaaa accacttctc attggcactg atgtttccct agcacgactg
     3301 cagaaagcag atgttgttgc ccagtcaagg atctccttcc ctgagaaaga gctgcttttg
     3361 ttgatacgaa accatcttat ttctaaaggg cttggagaaa cagcaaccgt gctgacaaaa
     3421 gaggctgacc tgcccatgac tgctgcctcc cattcttctg cctttacccc agtcactgct
     3481 gctgcttctc ctgtctctct accccgaacc cctcgtatcg ctaatggcat tgcaactcgt
     3541 ctgggcagcc atgctgctgt gggtgcctct gcgccttctg cccctactgc tcatcctcag
     3601 ccacggcccc cccagggtcc gctagctctg cccggcccat cttatgcagg caactcccct
     3661 ttgattggta gaatcagttt tatcagagag aggccatcac cctgcaatgg caggaaaatc
     3721 agagtgttgc ggcagaagtc ggaccatggt gcctacagcc aaagcccagc cataaaaaaa
     3781 cagctggaca gacatcttcc ttccccacct acgctggaca gtataatcac agagtatctt
     3841 agagaacaac atgctcgctg caagaatcca gttgccacct gcccaccttt ctccctcttt
     3901 actcctcacc aatgtcctga gccaaaacag aggcggcaag cgccaataaa ctttacgtca
     3961 aggctaaacc gcagggcatc atttccaaag tatggagggg tggatggcgg atgctttgat
     4021 aggcacctta tctttagcag attccgtcct atttcagtgt tccgggaagc caatgaagat
     4081 gagagtggct tcacctgctg tgcattctca gcacgggagc ggttcctgat gcttggcacc
     4141 tgcacagggc agctgaagct ctataatgtg tttagtggac aggaggaggc cagctataac
     4201 tgtcacaact cagccatcac acatcttgaa ccttccaggg atgggtcctt gctgctgaca
     4261 tctgctactt ggagccagcc tttgtctgca ctttggggaa tgaagtcagt atttgatatg
     4321 aagcattcct tcacagaaga tcactatgtt gagttcagta agcactccca ggatcgggtc
     4381 atcggcacaa aaggagacat tgcccacatt tatgatattc agactggcaa caagctgttg
     4441 actctgttta acccagatct tgccaacaac tacaagagga actgtgccac ctttaatcct
     4501 acagatgatc ttgtcttaaa tgatggcgtc ctctgggatg tccgctctgc acaggccatc
     4561 cacaagtttg acaagttcaa tatgaacatc agtggtgttt tccatccaaa tggactggag
     4621 gtgatcatta atactgagat ttgggacctt cgaacttttc atcttttgca tactgttccc
     4681 gctctggatc agtgtcgcgt ggtgttcaat cacacgggaa cagtgatgta tggagctatg
     4741 ttgcaggcag atgatgaaga tgacttaatg gaagagagga tgaaaagccc ctttgggtca
     4801 tccttccgaa catttaatgc aactgactac aaacctatag caaccattga tgtgaaacgg
     4861 aacatctttg acctgtgtac agacaccaaa gactgctatc ttgctgtcat tgagaatcaa
     4921 ggcagcatgg atgccctgaa catggacaca gtatgcaggc tgtatgaagt gggcaggcag
     4981 cgtctggcag aggatgagga tgaagaggag gaccaggaag aggaagaaca ggaggaagaa
     5041 gatgatgatg aagatgatga tgacaccgat gatttagatg agcttgacac tgaccagttg
     5101 ctggaggcgg agttggagga ggacgacaat aatgagaacg caggggaaga tggggacaat
     5161 gacttctctc cctctgatga ggagctagca aaccttctag aggagggaga ggacggggag
     5221 gatgaagact ctgatgcaga tgaggaggtg gaactgatct tgggggacac tgacagctct
     5281 gacaactctg atttggaaga tgacatcatc ttatctctga atgagcccgg gctcgagata
     5341 tctagaccca gctttcttgt acaaagtggt gatatcctcg agcatgcatc tagagggccc
     5401 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     5461 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5521 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5581 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5641 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5701 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5761 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5821 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5881 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5941 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6001 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6061 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6121 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6241 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6301 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6361 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6421 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6481 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     6541 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6601 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6661 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6721 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6781 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6841 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6901 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6961 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     7021 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     7081 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7141 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7201 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7261 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7321 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     7381 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7441 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7501 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7621 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7681 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7741 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7801 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7861 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7981 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8281 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8521 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8701 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8761 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8821 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8881 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8941 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9001 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9061 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9121 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9181 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9241 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9301 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9361 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9421 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9481 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9541 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9601 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9661 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9721 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9781 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9841 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9901 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9961 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    10021 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    10081 gcgtt
//
