LOCUS       sequence-261717-        6642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FLAG-tagged ODC1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, 
            Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM
  TITLE     MCART1/SLC25A51 is required for mitochondrial NAD transport.
  JOURNAL   Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. 
            Print 2020 Oct.
  PUBMED    33087354
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-21"; volume: "6(43):eabe5310. doi"; pages: " 
            10.1126/sciadv.abe5310. Print 2020 Oct"
COMMENT     Sequence Label: pMXs_FLAG-ODC1
FEATURES             Location/Qualifiers
     source          1..6642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             89..505
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     435..457
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     473..489
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    515..889
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     827..843
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..992
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(1989..2563,2564..2566,2567..2575)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2156..2173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2383..2402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2576..2971
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3142..3611
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3741..3758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3912..4500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3992..4011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4664..5455,5456..5524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5287..5306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5525..5629)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5659..6252
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6315..6642,1..30)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
       61 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
      121 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
      181 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
      241 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
      301 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
      361 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
      421 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
      481 agccctcact ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc
      541 cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct
      601 ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc
      661 agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac
      721 agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac
      781 agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc
      841 cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc cggatctagc
      901 tagttaatta aggatccgcc accatggact acaaggacca cgacggtgac tacaaggacc
      961 acgacatcga ctacaaggac gacgacgaca aggggcatgg caccggcagc accggcagcg
     1021 aattcatgac ttctatagat aaccgcccgc tcccgttcat ttatcaattt acagccggag
     1081 caatcgcagg agtctccgag ctgcttgtaa tgtaccccct tgacgttgta aaaacgagga
     1141 tgcagcttca agtaacaacc aaggggcatc cagccgtagt cgccgctaag gccgctgtgg
     1201 atcattacac cggcgtaatg gactgcctga cgaagattgt aaagaaggag ggtttcagcc
     1261 acctttataa aggcattact tcaccaatac tgatggaggc gccaaaaagg gcgattaagt
     1321 tttcaggtaa cgacactttt caaacatttt acaagaaaat ctttccaacc cccaatgggg
     1381 agatgactca aaagatcgca atttactctg gtgcatccgc tggagcagtg gaggcgttcg
     1441 tagtagctcc gtttgaactg gtgaagatcc gcctgcaaga tgtaaatagc cagttcaaaa
     1501 cgcccattga agtggtcaag aacagtgtgg ttaaaggcgg agttctctcc ctttttaacg
     1561 gcctcgaagc cacgatatgg cggcacgtcc tctggaatgc cgggtatttc gggattattt
     1621 ttcaaattcg aaaattgctt cctgctgcaa aaacctccac ggaaaagaca cggaacgact
     1681 tgatagcggg agcgattggt ggcacggttg gctgtctgct caatacaccc tttgatgtgg
     1741 taaaaagccg aatacagcgc agtagcggac ctcttcggaa gtataattgg agcctgccct
     1801 cagttctctt ggtctatcga gaagaggggt tcaaagcgct gtataagggg tttgcaccaa
     1861 aagtaatgcg gctggctccc ggtgggggtc tcctgttggt tgtcttcacc aatgttatgg
     1921 acttttttcg ggaggtaaaa tacggtaaaa agcaatgagc ggccgctacg taaattccgc
     1981 cccccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2041 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2101 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2161 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2221 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2281 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2341 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2401 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2461 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2521 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     2581 tcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     2641 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     2701 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     2761 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     2821 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     2881 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     2941 gctgccctct ggttatgtgt gggagggcta agtcgacgat aaaataaaag attttattta
     3001 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     3061 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     3121 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     3181 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     3241 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     3301 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     3361 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     3421 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     3481 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     3541 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     3601 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
     3661 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4321 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc
     4621 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5641 gaaaagtgcc acctgcataa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     5701 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag
     5761 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     5821 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     5881 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     5941 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     6001 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     6061 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     6121 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     6181 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     6241 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     6301 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     6361 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     6421 acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg
     6481 gacttcgggg gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact
     6541 ctttggtgca cccccctaat aggagggata tgtggttctg gtaggagacg agaacctaaa
     6601 acagttcccg cctccgtctg aatttttgct ttcggtttgg ga
//
