LOCUS       sequence-26172-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-myc3-NEDD8
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(522..540)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(523..540)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(560..577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    566..790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atggagcaaa agctcatctc agaggaagat ctggaattag
      121 agcaaaagct catctcagag gaagatctgg aattagagca aaagctcatc tcagaggaag
      181 atctggaatt caggatcctg atatccgcgg taccaagctt ggtaccgtta acatggatct
      241 acatgctaat taaagtgaag acgctgaccg gaaaggagat tgagattgac attgaaccta
      301 cagacaaggt ggagcgaatc aaggagcgtg tggaggagaa agagggaatc cccccacaac
      361 agcagaggct catctacagt ggcaagcaga tgagtgatga gaagacagca gctgattaca
      421 agattttagg tggttcagtc cttcacctgg tgttggctct gagaggagga ggtggtctta
      481 ggcagtgatg gaccctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
      541 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
      721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
      841 ccctgtagcg gcgcattaa
//
