LOCUS       sequence-261722-        6017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein mKate(KPS12) variant for optimized expression in
            Bacillus mycoides; under control of mannose inducible promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Yi Y, Frenzel E, Spoelder J, Elzenga JTM, van Elsas JD, Kuipers OP
  TITLE     Optimized fluorescent proteins for the rhizosphere-associated 
            bacterium Bacillus mycoides with endophytic and biocontrol agent 
            potential.
  JOURNAL   Environ Microbiol Rep. 2018 Feb;10(1):57-74. doi: 
            10.1111/1758-2229.12607. Epub 2018 Jan 5.
  PUBMED    29195004
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1758-2229.12607"; journalName: "Environ Microbiol Rep"; 
            date: "2018-02"; volume: "10"; issue: "1"; pages: "57-74"
COMMENT     Sequence Label: pAD-KPS12-Pman
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(981..1000)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    3867..4007
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4022..4039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4193..4781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4273..4292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4952..5743,5744..5812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5575..5594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5813..5917)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaattcgcag ggattattcc ttgctttttt tgttataggg aaaaatgcct ttattaccgg
       61 aacctatggt aaaaaaagcg attttaatga gctgatttcg gtatacagtt gagacatcta
      121 gaaggaaaat taatgtcaga acttatcaag gaaaatatgc acatgaaatt gtacatggaa
      181 ggaacagtaa ataatcacca ctttaaatgt acctcagaag gagaaggaaa accatatgaa
      241 ggtactcaaa ccatgcgtat taaggccgtt gaaggtggac cattgccttt tgcctttgat
      301 attcttgcta catcttttat gtacggatca aagactttta tcaatcatac ccaaggtatc
      361 ccagatttct ttaaacagtc atttcctgaa ggatttacat gggaacgtgt cacaacttat
      421 gaagatggtg gagtattgac agcaactcaa gatacatctt tgcaagatgg ttgtcttatc
      481 tacaatgtaa agatccgtgg agttaatttt ccaagtaatg gtcctgttat gcagaaaaag
      541 acccttggat gggaagcatc taccgaaaca ttatatcctg ccgatggtgg attggaaggt
      601 cgtgctgata tggcattgaa acttgtcggt ggaggtcacc ttatctgtaa tttgaagacc
      661 acataccgtt ctaaaaagcc agctaaaaat cttaagatgc ctggtgttta ctacgtcgat
      721 cgtcgtttag aacgtatcaa agaagcatat aaggaaactt atgttgaaca gcacgaagta
      781 gccgtcgcac ggtattgtga tttgcctagt aaattgggac accgttaata ataaaagctt
      841 gagaaggcat gcaagcttga gtaggacaaa tccgccgagc ttcgacgaga ttttcaggag
      901 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      961 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1021 ccgttcagaa caaagaatac aagaaaatat ttacaaaaaa tcaatttaac aattccttaa
     1081 aacatgcagg aattgacgat ttaaacaata ttagctttga acaattctta tctcttttca
     1141 atagctataa attatttaat aagtaagtta agggatgcat aaactgcatc ccttaacttg
     1201 tttttcgtgt gcctattttt tgtgaatcgc taagaaacca ttattatcat gacattaacc
     1261 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     1321 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1381 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     1441 tatgcggcat cagagcagat tgtactgaga gtgcaccata caaaacatat ttcaacacaa
     1501 tacaaatggg ttagttaaaa aagcaggcct tctaaaggtc tgcttttttt atttgattat
     1561 gtaattttta atgccaggat gccaataagc cataacctca aatgcaccat ttgcaacctc
     1621 gtcatcttct tcctcaatct tgaccagatc gccgtcctcc gcatcaccaa gattcagctc
     1681 tttatgtatc tccttcaaaa tgccaccgta tccaattaac cttcgagctg ccaacgcatc
     1741 atccaagtaa agcaccgtgt tcagattgtc ttcagtcacc ttattaccgc gcacaacatc
     1801 cgtatcctta accggatatt tagagatttc gagaacagct ttttgctcca tcattgcgtt
     1861 ccgcacatcg ttttcaatct gttcagcgtc aatcttagct ttacctttca ctcgacgaat
     1921 atcgacaatt ggagtgtaat ccaatttcat cgcctttttc caaaggctcg tccactccgc
     1981 ctgcttaata tagtttttcc caaaataatt tttccttact ggtatcaaca catgaaaatg
     2041 aggatgatat gtatcttctt catgattttt ggtaatctct aaagctctga aaaatccaag
     2101 aaccgaagtt tttacttttt tgtactggaa cagtttccta aagccttcca tcatcgcaga
     2161 aatttgtggc ttcagccgtt ctccctttac atttcgaatc gtcagcgtga gaaaaatcca
     2221 tccgcagccg tactgtctat tggcttcctc tacgatcaac ttattgtgat aagcaatttt
     2281 taacgacctg cgccacgcac acatcggaca taacctcact ttacaaaaat gggcttgata
     2341 cagttttaac ttgcccgtct ccgggtctct cttaaacgaa agatactctg cacaactaat
     2401 tagtttttca gcctttttgc catagtaagg tgccccaatc ttactctcta acgcttcgta
     2461 atgctccgcc atgaggttcg tccgtctctt tttccccttc caatcccgct ttttacctgt
     2521 tgcggtttta tcttcgagga tgctataatc attttcagat gaataaatca acaaaaaaac
     2581 tccttctgag ctagttctct agcattctat tattttgatt cgacacctta ataatagcag
     2641 aaggagtttt tacctgtcaa agaaccatca aacccttgat acacaaggct ttgacctaat
     2701 tttgaaaaat gatgttgttt ctatatagta tcaagataag aaagaaaagg atttttcgct
     2761 acgctcaaat cctttaaaaa aacacaaaag accacatttt ttaatgtggt cttttattct
     2821 tcaactaaag cacccattag ttcaacaaac gaaaattgga taaagtggga tatttttaaa
     2881 atatatattt atgttacagt aatattgact tttaaaaaag gattgattct aatgaagaaa
     2941 gcagacaagt aagcctccta aattcacttt agataaaaat ttaggaggca tatcaaatga
     3001 actttaataa aattgattta gacaattgga agagaaaaga gatatttaat cattatttga
     3061 accaacaaac gacttttagt ataaccacag aaattgatat tagtgtttta taccgaaaca
     3121 taaaacaaga aggatataaa ttttaccctg catttatttt cttagtgaca agggtgataa
     3181 actcaaatac agcttttaga actggttaca atagcgacgg agagttaggt tattgggata
     3241 agttagagcc actttataca atttttgatg gtgtatctaa aacattctct ggtatttgga
     3301 ctcctgtaaa gaatgacttc aaagagtttt atgatttata cctttctgat gtagagaaat
     3361 ataatggttc ggggaaattg tttcccaaaa cacctatacc tgaaaatgct ttttctcttt
     3421 ctattattcc atggacttca tttactgggt ttaacttaaa tatcaataat aatagtaatt
     3481 accttctacc cattattaca gcaggaaaat tcattaataa aggtaattca atatatttac
     3541 cgctatcttt acaggtacat cattctgttt gtgatggtta tcatgcagga ttgtttatga
     3601 actctattca ggaattgtca gataggccta atgactggct tttataatat gagataatgc
     3661 cgactgtact ttttacagtc ggttttctaa tgtcactaac ctgccccgtt agtcgccatt
     3721 cgccactgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3781 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3841 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     3901 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3961 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     4021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4621 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     5281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     5581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5941 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     6001 ggccctttcg tcttcaa
//
