LOCUS       sequence-261754-        9212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2014..2079
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2089..2677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2142..2162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2560..2571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2691..3190
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2726..2749)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3123..3142
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3211..3810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3211..3230)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3707..3727
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3948..4181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4253..4374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4290..4309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4344..4363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4414..4549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4569..4588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4570..4588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4598..4615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4598..4614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4607..4629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4756..5211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4843..4862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5053..5074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5237..5341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5342..5410,5411..6202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5560..5579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6373..6961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6862..6881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7115..7132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7249..7270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7285..7290,7291..7308,7309..7315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7323..7339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7328..7350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7347..7363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7347..7363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7382..7402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7384..7402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7430..7656
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7657..7837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7884..8009
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8502..8735
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8920..8964
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat gactcccata cgtaagtgta atgactcatt gcgtcattca
     1921 ccaaaggttg agacgacaga ttgtccagtt cctcctaaaa gaatgagacg agaagctaca
     1981 agacaaaata ggattataac caaaacagat gtagactaca aagaccatga cggtgattat
     2041 aaagatcatg acatcgatta caaggatgac gatgacaagt gagtcgacaa tcaacctctg
     2101 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2161 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2221 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2281 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2341 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2461 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     2521 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2581 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2641 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     2701 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     2761 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     2821 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     2881 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     2941 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     3001 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     3061 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     3121 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     3181 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     3241 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3301 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     3361 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3421 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3481 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     3541 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     3601 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     3661 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     3721 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     3781 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     3841 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     3901 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     3961 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4021 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4081 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4141 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     4201 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     4261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4381 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4441 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4501 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     4561 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     4621 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     4681 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4741 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4801 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4861 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4921 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4981 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5041 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5101 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5161 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     5221 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5281 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5341 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5401 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5461 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5521 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5581 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5641 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5701 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5761 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5821 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5881 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5941 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6001 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6061 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6121 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6181 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6241 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6301 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6361 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6421 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6481 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6541 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6601 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6661 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6721 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6781 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6841 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6901 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6961 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7021 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7081 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7141 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7201 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7261 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7321 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     7381 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     7441 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     7501 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     7561 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     7621 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     7681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7801 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7861 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7921 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7981 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8041 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8101 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8161 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8221 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8281 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8341 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8401 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     8461 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     8521 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     8581 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     8641 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8701 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8761 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8821 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8881 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8941 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9001 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9061 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9121 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     9181 agagacagat ccattcgatt agtgaacgga tc
//
