LOCUS       sequence-261764-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NuperNova fused to trans-Golgi protein TGON3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Paardekooper LM, van Vroonhoven E, Ter Beest M, van den Bogaart G
  TITLE     Radical Stress Is More Cytotoxic in the Nucleus than in Other 
            Organelles.
  JOURNAL   Int J Mol Sci. 2019 Aug 25;20(17). pii: ijms20174147. doi: 
            10.3390/ijms20174147.
  PUBMED    31450682
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2019-08-25"; volume: "20(17). pii: ijms20174147. doi";
            pages: " 10.3390/ijms20174147"
COMMENT     Sequence Label: SuperNova-TGON3
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(612..617,618..1325)
                     /codon_start=1
                     /product="monomeric variant of the photosensitizing red 
                     fluorescent protein KillerRed (Takemoto et al., 2013)"
                     /label=SuperNova
                     /translation="GSEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNV
                     HAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDGT
                     MTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTAT
                     IGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVAY
                     AHSVPRITSAIGSDED"
     polyA_signal    2532..2653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2569..2588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2623..2642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2660..3115)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2797..2818)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3009..3028
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3142..3246
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3248..3605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3248..3439
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3271..3291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3456..3591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3640..4434
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3640..4434
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3694..3713)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4304..4323
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4622..4641)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4666..4713
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5042..5630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5531..5550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 tggtaaagct tggttcagag gtgggccccg ccctgttcca gagcgacatg accttcaaaa
      661 tcttcatcga cggcgaggtg aacggccaga agttcaccat cgtggccgac ggcagcagca
      721 agttccccca cggcgacttc aacgtgcacg ccgtgtgcga gaccggcaag ctgcccatga
      781 gctggaagcc catctgccac ctgatccagt acggcgagcc cttcttcgcc cgctaccccg
      841 acggcatcag ccatttcgcc caggagtgct tccccgaggg cctgagcatc gaccgcaccg
      901 tgcgcttcga gaacgacggc accatgacca gccaccacac ctacgagctg gacgacacct
      961 gcgtggtgag ccgcatcacc gtgaactgcg acggcttcca gcccgacggc cccatcatgc
     1021 gcgaccagct ggtggacatc ctgcccagcg agacccacat gttcccccac ggccccaacg
     1081 ccgtgcgcca gaccgccacc atcggcttca ccaccgccga cggcggcaag atgatgggcc
     1141 acttcgacag caagatgacc ttcaacggca gccgcgccat cgagatcccc ggcccacact
     1201 tcgtgaccat catcaccaag cagaccaggg acaccagcga caagcgcgac cacgtgtgcc
     1261 agcgcgaggt ggcctacgcc cacagcgtgc cccgcatcac cagcgccatc ggtagcgacg
     1321 aggattcgaa ttctatgcag ttcctggtgg ccctgctgct gctgagcgtg gccgtggccc
     1381 gcgccctgcc cagcgccagc aagcccaaca acaccagcag cgagaacaac ccccccatcc
     1441 agcccagcac ccccctgccc cccggcgtgg acatcagcca gcaggtgaag accaaccgcc
     1501 ccaccgacca gcgcctggag agcgacaagg agggccagga caagaccgtg gcccgcacca
     1561 gcgccagcgt gagcagcggc gtggagagcg ccaccaacct gaacctggac gacagcaaga
     1621 agcaccccga gaccgccgac gccaagctga aggagaccct gcagcagctg ctgcccgtgg
     1681 accccaagca ggagaagagc ggccagaagt tcaccaagga cagcggcagc cccaccggcg
     1741 gcgacagcga caacaccacc ggcggcgaca gcaacaagac caccggcgtg gacagcgaca
     1801 agaccagcgg cggcgacagc aacaagccca ccggcagcga caacgacaag cccaccggcg
     1861 gcgacagcaa caagcccacc agcaaggtgc ccagcaacac cgagaccccc aagatcgaca
     1921 aggtgcagct gaccgagaag ggccagaagc ccaccctgat cagcaagacc gagagcggcg
     1981 agaagctggc cggcgacagc gacttcagcc tgaagcccga gaagggcgac aagagcagcg
     2041 agcccaccga ggacgtggag accaaggaga tcgaggaggg cgacaccgag cccgaggagg
     2101 gcagccccct ggaggaggag aacgagaagg tgctgggccc cagcagcagc gagaaccagg
     2161 agggcaccct gaccgacagc atgaaggacg agaaggacga ccactacaag gacaacagcg
     2221 gcaacaccag cgccgagagc agccacttct tcgcctacct ggtgaccgcc gccgtgctgg
     2281 tggccgtgct gtacatcgcc taccacaaca agcgcaagat catcgccttc gccctggagg
     2341 gcaagcgcag caaggtgacc cgccgcccca aggccagcga ctaccagcgc ctgaacctga
     2401 agctgcggga tccacccggg atctagatca taatcagcca taccacattt gtagaggttt
     2461 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2521 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2581 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2641 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     2701 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2761 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2821 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     2881 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2941 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     3001 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3061 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     3121 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3241 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3301 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3421 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3481 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3541 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3601 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3661 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3721 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3781 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     3841 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3901 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3961 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4021 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4081 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4141 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     4201 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4261 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4321 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4381 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4441 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4501 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4561 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     4621 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4681 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4741 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4801 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4861 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     4921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5161 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5701 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
