LOCUS       sequence-261772-        9228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]
FEATURES             Location/Qualifiers
     source          1..9228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          164..736
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    791..815
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1769..2470)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     regulatory      2467..2476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2538..2741)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2567..2587)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2742..3045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3198..3207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4829..4847)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5097..5116
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          5462..6034
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    6231..6286
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6684..6701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6855..7443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6935..6954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7614..8405,8406..8474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8237..8256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8475..8579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8647..8665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    9140..9195
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 tgtccccata atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat
       61 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattctttgc
      121 caaaatgatg agacagcaca acaaccagca cgttgcccag gagctgtagg aaagagaaga
      181 aggcatgaac atggttagca gaggggcccg gtttggactc agagtatttt atcctcatct
      241 caaacagtgt atatcattgt aaccataaag agaaaggcag gatgatgacc agggtgtagt
      301 tgtttctacc aataagaata tttccacgcc agccagaatt tatatgcaga aatattctac
      361 cttatcattt aattataaca attgttctct aaaactgtgc tgaagtacaa tataatatac
      421 cctgattgcc ttgaaaaaaa agtgattaga gaaagtactt acaatctgac aaataaacaa
      481 aagtgaattt aaaaattcgt tacaaatgca agctaaagtt taacgaaaaa gttacagaaa
      541 atgaaaagaa aataagagga gacaatggtt gtcaacagag tagaaagtga aagaaacaaa
      601 attatcatga gggtccatgg tgatacaagg gacatcttcc cattctaaac aacaccctga
      661 aaactttgcc ccctccatat aacatgaatt ttacaatagc gaaaaagaaa gaacaatcaa
      721 gggtccccaa actcaccctg aagttctcag ctctagagat atcgcatgca agcttactag
      781 tagaggatca accactttgt acaagaaagc tgggtctagt ctacattccc cagccagaag
      841 acttagaatc catgcttgag ccaaagcctc cattaaaacc actgcccgac cctgcattgg
      901 atgctgatcc ccaaccaatt gctgcaccag aattagagcc actataagag ttatttccag
      961 aaccgaaggc ctggtttggc tccctctgca tgttgccttg gttttggtta ttacccgatg
     1021 ggcctgactg gttctgctgg ctggctaaca tgcccatcat accccaactg ctctgtagtg
     1081 ctgcctgggc ggcagccatc atggctggat taatgctgaa cgcaccaaag ttcatcccac
     1141 cacccatatt actaccttga ttgtttccca aaccagctcc accccctctg ctattaccaa
     1201 atccaccctg attcccaaag ccacctggat taccaccaaa tcttccactt ctttctaact
     1261 gtctattgct attgtgctta ggttcggcat tggatatatg aacgctgatt cctttaatga
     1321 tcaagtcctc tccacaaaga gactgcgcaa tctgatcatc tgcaaatgta acaaaggcaa
     1381 aggccctgaa tggcttgggg atgaagacat ccatcacatc cccgtactga gagaagaact
     1441 cccgcagctc atcctcagtc atgtcctctg tacagcgccc cacaaacact tttctgcttc
     1501 tcaaaggctc atcttggctt tgcttagaat taggaagttt gcagtcacac catcgtccat
     1561 ctatcatatg tcgctgtgac attactttca cttgtgtttc atattccgta aaacgaacaa
     1621 agccaaaccc ctttgaatga ccagtcttaa gatctttctt gacctgcacc ataagaactt
     1681 ctccaaaggt actaaaatac tctttcaggt cctgttcggt tgttttccat gggagaccca
     1741 acactattaa atcggatgtc attccggaat taagcttgtg ccccagtttg ctagggaggt
     1801 cgcagtatct ggccactgcc acctcgtgct gctcgacgta ggtctcgttg ttggcctcct
     1861 tgattctttc cagtctgtag tccacatagt agacgccagg catcttgagg ttcttagcgg
     1921 gtttcttgga tctatatgtg gtcttggcgt ttgcgatcag atggctcccg cccacgagct
     1981 tcagggccat gtcgtttctg ccttccaggc cgccgtcagc ggggtacagc gtctcggtga
     2041 aggcctccca gccgagtgtt ttcttctgca tcacagggcc gttggatgtg aagttcaccc
     2101 ctctgatctt gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg
     2161 tcagcacgcc cccgtcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg
     2221 actgcttgaa gaagtcgggg atgccctggg tgtggttgat gaaggtcttg ctgccgtaga
     2281 ggaagctagt agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga
     2341 ttctcatggt ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt
     2401 gatggttgtc cacggtgccc tccatgtaca gcttcatgtg catgttctcc ttaatcagct
     2461 cgcttcccat ggtggcaccg gtggtaacgc gtcagctgac tagaggatcc ctagcggatc
     2521 tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc
     2581 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     2641 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     2701 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     2761 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     2821 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     2881 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     2941 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     3001 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     3061 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactactcga gtatgtcgag
     3121 gtggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3181 ctggagaatt caccggtgcc accatgggat ccaccaacga gaatgctaat acaccagctg
     3241 cccgtcttca cagattcaag aacaagggaa aagacagtac agaaatgagg cgtcgcagaa
     3301 tagaggtcaa tgtggagctg aggaaagcta agaaggatga ccagatgctg aagaggagaa
     3361 atgtaagctc attccctgat gatggtacca gtaaaggaga ggaactattt acaggtactg
     3421 tgcctatcaa agtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgtggcg
     3481 agggcgaggg cgatgccacc gagggcaagc tgaccctgaa gttcatctgc accaccggca
     3541 agctgccggt gccgtggccg accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3601 gccgctaccc ggaccacatg aagcgtcacg acttcttcaa gtccgccatg ccggaaggct
     3661 acgtccagga gcgcaccatc gagttcaagg acgacggcac ctacaagacc cgcgcggagg
     3721 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcaac gacttcaagg
     3781 aggacggcaa catcctgggt cacaagctgg agtacaacca caacagccac aacgtccgta
     3841 tcgaagccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
     3901 aggacggcag ccagcaggaa gccgaccaca aacagcagaa caccccgatc ggcgacggcc
     3961 cggtgcgcct gccggacaac cactatctga gcacccagac cacgctgagc aaagacccga
     4021 acgagaagcg cgatcacatg gtcctgaagg agttcgtgac ggcagccggt atcactaaag
     4081 gtgaagatga aagtggaggc accggaacca gcaagggcga ggaggataac atggccatca
     4141 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     4201 agatcgaggg cgagggcgag gtgagtatgg tacataaaac aagcatctat tgaaaatatc
     4261 tgacaaactc atcttttatt tttgatgtgt gtgtgtgtgt gtgtgtgtgt gttttttttt
     4321 aacagggatt tggggaatta tttgagaaag caaaacaaaa caataacaat agaaaaactt
     4381 ctaatggtga tgacagcctc ttcttcagta atttctcact tcttggtact cctgtcctga
     4441 aagatattaa tttcaagata gaaagaggac agttgttggc ggttgctgga tccactggag
     4501 caggcaaggt agttcttttg ttcttcacta ttaagaactt aatttggtgt ccatgtctct
     4561 ttttttttct agtttgtagt gctggaaggt atttttggag aaattcttac atgagcatta
     4621 ggagaatgta tgggtgtagt gtcttgtata atagaaattg ttccactgat aatttactct
     4681 agttttttat ttcctcatat tattttcagt ggctttttct tccacatctt tatattttgc
     4741 accacattca acactgtatc ttgcacatgg cgagcatgcc ctttcctttt ttttcctcag
     4801 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4861 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     4921 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4981 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5041 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5101 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5161 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5221 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5281 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5341 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagctatc
     5401 tagacccagc tttcttgtac aaagtgattg atcctctcga ctctagactg agaacttcag
     5461 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
     5521 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
     5581 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
     5641 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
     5701 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     5761 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     5821 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     5881 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     5941 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     6001 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     6061 gctgtctcat cattttggca aagaattcac tcctcaggtg caggctgcct atcagaaggt
     6121 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     6181 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     6241 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     6301 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     6361 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     6421 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     6481 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     6541 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     6601 cttatggaga tccctcgact gcattaatga atcggccaac gcgcggggag aggcggtttg
     6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8221 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8581 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8641 tataaaaata ggcgtatcac gaggcccttt cgtcttcact cgacgcggcc agcttggtcg
     8701 agctggatac ttcccgtccg ccagggggac atgccggcga tgctgaaggt cgcgcgcatt
     8761 cccgatgaag aggccggtta ccgcctgttg acctggtggg acgggcaggg cgccgagttc
     8821 ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta
     8881 gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata
     8941 taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg
     9001 gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca gccagcatat
     9061 gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     9121 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     9181 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatga
//
