LOCUS       sequence-261779-        9504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BirA-GFP fusions  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Kulyyassov A, Ogryzko V
  TITLE     In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2
            and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation.
  JOURNAL   Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 
            10.3390/biom10010142.
  PUBMED    31963153
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2020-01-16"; volume: "10(1). pii: 
            biom10010142. doi"; pages: " 10.3390/biom10010142"
COMMENT     Sequence Label: pOz-humBirA-GFP
FEATURES             Location/Qualifiers
     source          1..9504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1772..2734
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             2741..2764
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             join(2771..2773,2774..3487)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2771..2773,2774..3484)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2813..2834)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3074..3093)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3421..3442
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3558..4107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3702..3719)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3929..3948
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             5198..5667
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6363..6380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6363..6379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6588..6607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6707..6729
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6767..6785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6853..6957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6958..7026,7027..7818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7176..7195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7989..8577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8478..8497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8731..8748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8865..8886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8901..8906,8907..8924,8925..8931)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8939..8955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8944..8966
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8963..8979
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8963..8979
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcctatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgcca gatcttagac catgaaggac aacaccgtgc ccctgaagct
     1801 gatcgccctg ctggccaacg gcgagttcca ctctggcgag cagctgggag agaccctggg
     1861 aatgagcaga gccgccatca acaagcacat ccagacactg agagactggg gagtggacgt
     1921 gttcaccgtg cctggcaagg gctacagcct gcctgagcct atccagctgc tgaacgccaa
     1981 gcagatcctg ggacagctgg atggcggaag cgtggccgtg ctgcctgtga tcgactccac
     2041 caatcagtac ctgctggaca gaatcggaga gctgaagtcc ggcgacgcct gcatcgccga
     2101 gtaccagcag gctggcagag gacgcagagg acggaagtgg ttcagcccat tcggagccaa
     2161 cctgtacctg tccatgttct ggagactgga gcagggacct gctgctgcca tcggactgag
     2221 tctggtgatc ggaatcgtga tggccgaggt gctgagaaag ctgggagccg acaaggtgag
     2281 agtgaagtgg cctaatgacc tgtacctcca ggaccgcaag ctggctggca tcctggtgga
     2341 gctgacaggc aagacaggcg atgccgctca gatcgtgatc ggagccggaa tcaacatggc
     2401 catgagaaga gtggaggaga gcgtggtgaa ccagggctgg atcaccctgc aggaggctgg
     2461 catcaacctg gaccggaaca ccctggccgc catgctgatc agagagctga gagccgctct
     2521 ggagctgttc gagcaggagg gactggctcc ttacctgagc agatgggaga agctggacaa
     2581 cttcatcaac agacctgtga agctgatcat cggcgacaag gaaatcttcg gcatctccag
     2641 aggaatcgac aagcagggag ctctgctgct ggagcaggac ggaatcatca agccctggat
     2701 gggcggagaa atctccctga gaagcgcaga gaagggagga catcatcacc atcaccacca
     2761 tcacctcgag gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2821 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2881 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2941 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3001 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3061 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3121 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3181 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3241 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3301 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3361 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3421 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3481 caagtagcgg ccgcatcgag agatccggga gacacaacgg tttccctcta gcgggatcaa
     3541 ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3601 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3661 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3721 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3781 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3841 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3901 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3961 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4021 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4081 ggttttcctt tgaaaaacac gataatacca tggattcata cctgctgatg tggggactgc
     4141 tcacgttcat catggtgcct ggctgccagg cagagctctg tgacgatgac ccgccagaga
     4201 tcccacacgc cacattcaaa gccatggcct acaaggaagg aaccatgttg aactgtgaat
     4261 gcaagagagg tttccgcaga ataaaaagcg ggtcactcta tatgctctgt acaggaaact
     4321 ctagccactc gtcctgggac aaccaatgtc aatgcacaag ctctgccact cggaacacaa
     4381 cgaaacaagt gacacctcaa cctgaagaac agaaagaaag gaaaaccaca gaaatgcaaa
     4441 gtccaatgca gccagtggac caagcgagcc ttccaggtca ctgcagggaa cctccaccat
     4501 gggaaaatga agccacagag agaatttatc atttcgtggt ggggcagatg gtttattatc
     4561 agtgcgtcca gggatacagg gctctacaca gaggtcctgc tgagagcgtc tgcaaaatga
     4621 cccacgggaa gacaaggtgg acccagcccc agctcatatg cacaggtgaa atggagacca
     4681 gtcagtttcc aggtgaagag aagcctcagg caagccccga aggccgtcct gagagtgaga
     4741 cttcctgcct cgtcacaaca acagattttc aaatacagac agaaatggct gcaaccatgg
     4801 agacgtccat atttacaaca gagtaccagg tagcagtggc cggctgtgtt ttcctgctga
     4861 tcagcgtcct cctcctgagt gggctcacct ggcagcggag acagaggaag agtagaagaa
     4921 caatctagag gatccggatt agtccaattt gttaaagaca ggatatcagt ggtccaggct
     4981 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa
     5041 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     5101 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     5161 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     5221 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     5281 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5341 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     5401 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5461 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     5521 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     5581 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     5641 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     5701 tttcttaagt atttacatta aatggccata gtacttaaag ttacattggc ttccttgaaa
     5761 taaacatgga gtattcagaa tgtgtcataa atatttctaa ttttaagata gtatctccat
     5821 tggctttcta ctttttcttt tatttttttt tgtcctctgt cttccatttg ttgttgttgt
     5881 tgtttgtttg tttgtttgtt ggttggttgg ttaatttttt tttaaagatc ctacactata
     5941 gttcaagcta gactattagc tactctgtaa cccagggtga ccttgaagtc atgggtagcc
     6001 tgctgtttta gccttcccac atctaagatt acaggtatga gctatcattt ttggtatatt
     6061 gattgattga ttgattgatg tgtgtgtgtg tgattgtgtt tgtgtgtgtg attgtgtata
     6121 tgtgtgtatg gttgtgtgtg attgtgtgta tgtatgtttg tgtgtgattg tgtgtgtgtg
     6181 attgtgcatg tgtgtgtgtg tgattgtgtt tatgtgtatg attgtgtgtg tgtgtgtgtg
     6241 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt gtgtatatat atttatggta gtgagaggca
     6301 acgctccggc tcaggtgtca ggttggtttt tgagacagag tctttcactt agcttggaat
     6361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     6541 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     6601 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6661 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6721 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     6781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7201 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7501 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8701 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8761 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8821 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8881 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8941 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctttgc
     9001 tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga cttgttctat
     9061 gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat gttaattcca
     9121 ttttaaatgc acagatgttt ttatttcata agggtttcaa tgtgcatgaa tgctgcaata
     9181 ttcctgttac caaagctagt ataaataaaa atagataaac gtggaaatta cttagagttt
     9241 ctgtcattaa cgtttccttc ctcagttgac aacataaatg cgctgctgag aagccagttt
     9301 gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt caattagttg
     9361 atttttattt ttgacatata catgtgaaag accccacctg taggtttggc aagctagctt
     9421 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
     9481 caaggtcagg aacagatgga acag
//
