LOCUS       sequence-261780-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: SmoM2 (W535L)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1539..1749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1561..1757
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1608..1743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1789..2391
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(1789..1808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2285..2305
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2609..3109
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3028..3046
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    3130..3154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5534..5558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5566..5607
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5632..6220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5685..5705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6103..6114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6292..6525
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6603..6724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6640..6659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6694..6713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6764..6899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6919..6938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6920..6938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6948..6965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6948..6964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6957..6979)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7106..7561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7193..7212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7403..7424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7587..7691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7692..7760,7761..8552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7910..7929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8723..9311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9212..9231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9465..9482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9599..9620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9635..9640,9641..9658,9659..9665)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9673..9689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9678..9700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9697..9713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9697..9713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9732..9752
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9734..9752
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9780..10006
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
     1801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     1861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     1921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     1981 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     2041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     2101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     2161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     2221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     2281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     2341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
     2401 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2581 actttggcgc cggctcgagg cctgcaggtt gcgccttttc caaggcagcc ctgggtttgc
     2641 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     2701 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     2761 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     2821 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     2881 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     2941 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     3001 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     3061 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg ggctagcatc
     3121 gatggatcaa caagtttgta caaaaaagtt ggctgtggcg gcggtggcac catggccgct
     3181 gcccgcccag cgcgggggcc ggagctcccg ctcctggggc tgctgctgct gctgctgctg
     3241 ggggacccgg gccggggggc ggcctcgagc gggaacgcga ccgggcctgg gcctcggagc
     3301 gcgggcggga gcgcgaggag gagcgcggcg gtgactggcc ctccgccgcc gctgagccac
     3361 tgcggccggg ctgccccctg tgagccgctg cgctacaacg tgtgcctggg ctcggtgctg
     3421 ccctacgggg ccacctccac actgctggcc ggagactcgg actcccagga ggaagcgcac
     3481 ggcaagctcg tgctctggtc gggcctccgg aatgcccccc gctgctgggc agtgatccag
     3541 cccctgctgt gtgccgtata catgcccaag tgtgagaatg accgggtgga gctgcccagc
     3601 cgtaccctct gccaggccac ccgaggcccc tgtgccatcg tggagaggga gcggggctgg
     3661 cctgacttcc tgcgctgcac tcctgaccgc ttccctgaag gctgcacgaa tgaggtgcag
     3721 aacatcaagt tcaacagttc aggccagtgc gaagtgccct tggttcggac agacaacccc
     3781 aagagctggt acgaggacgt ggagggctgc ggcatccagt gccagaaccc gctcttcaca
     3841 gaggctgagc accaggacat gcacagctac atcgcggcct tcggggccgt cacgggcctc
     3901 tgcacgctct tcaccctggc cacattcgtg gctgactggc ggaactcgaa tcgctaccct
     3961 gctgttattc tcttctacgt caatgcgtgc ttctttgtgg gcagcattgg ctggctggcc
     4021 cagttcatgg atggtgcccg ccgagagatc gtctgccgtg cagatggcac catgaggctt
     4081 ggggagccca cctccaatga gactctgtcc tgcgtcatca tctttgtcat cgtgtactac
     4141 gccctgatgg ctggtgtggt ttggtttgtg gtcctcacct atgcctggca cacttccttc
     4201 aaagccctgg gcaccaccta ccagcctctc tcgggcaaga cctcctactt ccacctgctc
     4261 acctggtcac tcccctttgt cctcactgtg gcaatccttg ctgtggcgca ggtggatggg
     4321 gactctgtga gtggcatttg ttttgtgggc tacaagaact accgataccg tgcgggcttc
     4381 gtgctggccc caatcggcct ggtgctcatc gtgggaggct acttcctcat ccgaggagtc
     4441 atgactctgt tctccatcaa gagcaaccac cccgggctgc tgagtgagaa ggctgccagc
     4501 aagatcaacg agaccatgct gcgcctgggc atttttggct tcctggcctt tggctttgtg
     4561 ctcattacct tcagctgcca cttctacgac ttcttcaacc aggctgagtg ggagcgcagc
     4621 ttccgggact atgtgctatg tcaggccaat gtgaccatcg ggctgcccac caagcagccc
     4681 atccctgact gtgagatcaa gaatcgcccg agccttctgg tggagaagat caacctgttt
     4741 gccatgtttg gaactggcat cgccatgagc accttggtct ggaccaaggc cacgctgctc
     4801 atctggaggc gtacctggtg caggttgact gggcagagtg acgatgagcc aaagcggatc
     4861 aagaagagca agatgattgc caaggccttc tctaagcggc acgagctcct gcagaaccca
     4921 ggccaggagc tgtccttcag catgcacact gtgtcccacg acgggcccgt ggcgggcttg
     4981 gcctttgacc tcaatgagcc ctcagctgat gtctcctctg cctgggccca gcatgtcacc
     5041 aagatggtgg ctcggagagg agccatactg ccccaggata tttctgtcac ccctgtggca
     5101 actccagtgc ccccagagga acaagccaac ctgtggctgg ttgaggcaga gatctcccca
     5161 gagctgcaga agcgcctggg ccggaagaag aagaggagga agaggaagaa ggaggtgtgc
     5221 ccgctggcgc cgccccctga gcttcacccc cctgcccctg cccccagtac cattcctcga
     5281 ctgcctcagc tgccccggca gaaatgcctg gtggctgcag gtgcctgggg agctggggac
     5341 tcttgccgac agggagcgtg gaccctggtc tccaacccat tctgcccaga gcccagtccc
     5401 cctcaggatc catttctgcc cagtgcaccg gcccccgtgg catgggctca tggccgccga
     5461 cagggcctgg ggcctattca ctcccgcacc aacctgatgg acacagaact catggatgca
     5521 gactcggact tcttgccaac tttcttgtac aaagtggtta ccggtggtaa gcctatccct
     5581 aaccctctcc tcggtctcga ttctacgtag taatgaccgc gttaagtcga caatcaacct
     5641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6001 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6181 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     6241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6301 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     6541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6721 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6841 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6901 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6961 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7021 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7081 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7141 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7201 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7261 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7321 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7381 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7441 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7501 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7561 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9721 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     9781 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     9841 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     9901 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     9961 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaac
//
